ID   RIBU_LEUMM              Reviewed;         196 AA.
AC   Q03WN0;
DT   31-MAY-2011, integrated into UniProtKB/Swiss-Prot.
DT   14-NOV-2006, sequence version 1.
DT   25-MAY-2022, entry version 77.
DE   RecName: Full=Riboflavin transporter RibU;
DE   AltName: Full=Riboflavin ECF transporter S component RibU;
GN   Name=ribU; OrderedLocusNames=LEUM_1295;
OS   Leuconostoc mesenteroides subsp. mesenteroides (strain ATCC 8293 / DSM
OS   20343 / BCRC 11652 / CCM 1803 / JCM 6124 / NCDO 523 / NBRC 100496 / NCIMB
OS   8023 / NCTC 12954 / NRRL B-1118 / 37Y).
OC   Bacteria; Firmicutes; Bacilli; Lactobacillales; Lactobacillaceae;
OC   Leuconostoc.
OX   NCBI_TaxID=203120;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 8293 / DSM 20343 / BCRC 11652 / CCM 1803 / JCM 6124 / NCDO 523
RC   / NBRC 100496 / NCIMB 8023 / NCTC 12954 / NRRL B-1118 / 37Y;
RX   PubMed=17030793; DOI=10.1073/pnas.0607117103;
RA   Makarova K.S., Slesarev A., Wolf Y.I., Sorokin A., Mirkin B., Koonin E.V.,
RA   Pavlov A., Pavlova N., Karamychev V., Polouchine N., Shakhova V.,
RA   Grigoriev I., Lou Y., Rohksar D., Lucas S., Huang K., Goodstein D.M.,
RA   Hawkins T., Plengvidhya V., Welker D., Hughes J., Goh Y., Benson A.,
RA   Baldwin K., Lee J.-H., Diaz-Muniz I., Dosti B., Smeianov V., Wechter W.,
RA   Barabote R., Lorca G., Altermann E., Barrangou R., Ganesan B., Xie Y.,
RA   Rawsthorne H., Tamir D., Parker C., Breidt F., Broadbent J.R., Hutkins R.,
RA   O'Sullivan D., Steele J., Unlu G., Saier M.H. Jr., Klaenhammer T.,
RA   Richardson P., Kozyavkin S., Weimer B.C., Mills D.A.;
RT   "Comparative genomics of the lactic acid bacteria.";
RL   Proc. Natl. Acad. Sci. U.S.A. 103:15611-15616(2006).
RN   [2]
RP   SUBUNIT, SUBCELLULAR LOCATION, AND EXPRESSION IN E.COLI.
RC   STRAIN=ATCC 8293 / DSM 20343 / BCRC 11652 / CCM 1803 / JCM 6124 / NCDO 523
RC   / NBRC 100496 / NCIMB 8023 / NCTC 12954 / NRRL B-1118 / 37Y;
RX   PubMed=18931129; DOI=10.1128/jb.01208-08;
RA   Rodionov D.A., Hebbeln P., Eudes A., ter Beek J., Rodionova I.A.,
RA   Erkens G.B., Slotboom D.J., Gelfand M.S., Osterman A.L., Hanson A.D.,
RA   Eitinger T.;
RT   "A novel class of modular transporters for vitamins in prokaryotes.";
RL   J. Bacteriol. 191:42-51(2009).
CC   -!- FUNCTION: Probable riboflavin-binding protein that interacts with the
CC       energy-coupling factor (ECF) ABC-transporter complex. Unlike classic
CC       ABC transporters this ECF transporter provides the energy necessary to
CC       transport a number of different substrates. The substrates themselves
CC       are bound by transmembrane, not extracytoplasmic soluble proteins and
CC       transport it into cells (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: In E.coli forms a stable energy-coupling factor (ECF)
CC       transporter complex probably composed of a membrane-embedded substrate-
CC       binding protein (S component), 2 ATP-binding proteins (A components)
CC       and 2 transmembrane proteins (T component). May be able to interact
CC       with more than 1 S component at a time. {ECO:0000269|PubMed:18931129}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC       protein {ECO:0000305}.
CC   -!- DOMAIN: Riboflavin is stacked with one or more Trp residues in the
CC       binding pocket of RibU. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the prokaryotic riboflavin transporter (P-RFT)
CC       (TC 2.A.87) family. {ECO:0000305}.
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DR   EMBL; CP000414; ABJ62392.1; -; Genomic_DNA.
DR   RefSeq; WP_011680010.1; NC_008531.1.
DR   AlphaFoldDB; Q03WN0; -.
DR   SMR; Q03WN0; -.
DR   STRING; 203120.LEUM_1295; -.
DR   EnsemblBacteria; ABJ62392; ABJ62392; LEUM_1295.
DR   GeneID; 61176279; -.
DR   KEGG; lme:LEUM_1295; -.
DR   eggNOG; COG3601; Bacteria.
DR   HOGENOM; CLU_086673_2_1_9; -.
DR   OMA; MFLEFPI; -.
DR   OrthoDB; 1497256at2; -.
DR   Proteomes; UP000000362; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0032217; F:riboflavin transmembrane transporter activity; IEA:InterPro.
DR   InterPro; IPR024529; ECF_trnsprt_substrate-spec.
DR   InterPro; IPR025720; RibU.
DR   PANTHER; PTHR38438; PTHR38438; 1.
DR   Pfam; PF12822; ECF_trnsprt; 1.
DR   PIRSF; PIRSF037778; UCP037778_transp_RibU; 1.
PE   1: Evidence at protein level;
KW   Cell membrane; Membrane; Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..196
FT                   /note="Riboflavin transporter RibU"
FT                   /id="PRO_0000409007"
FT   TRANSMEM        14..34
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        46..66
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        80..100
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        120..140
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        164..184
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   196 AA;  22268 MW;  E01415EBE6AD9956 CRC64;
     MSIIPVTRVQ RTTLIAILSA ISFGLMLFPQ VPIIPSADFL KLDFSIVPVV IGLYWLNYSA
     SLWVILIRTL LKLILANEGV NTYLGLPVNL LVVLAFITVL KITMPNLEQY SNWQKKILPL
     ISSTFVMTIV AIVINWFVAI PLYARFANFD IAKFIGLKNY FIGMVLPFNL IEGIIWFVVS
     MIILRAIQPL QRRFHS