RIBU_LISMO
ID RIBU_LISMO Reviewed; 203 AA.
AC Q8Y5W0;
DT 26-FEB-2020, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2002, sequence version 1.
DT 03-AUG-2022, entry version 91.
DE RecName: Full=Riboflavin transporter RibU {ECO:0000303|PubMed:27672192};
GN Name=ribU {ECO:0000303|PubMed:27672192};
GN OrderedLocusNames=lmo1945 {ECO:0000312|EMBL:CAD00023.1};
OS Listeria monocytogenes serovar 1/2a (strain ATCC BAA-679 / EGD-e).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Listeriaceae; Listeria.
OX NCBI_TaxID=169963;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC BAA-679 / EGD-e;
RX PubMed=11679669; DOI=10.1126/science.1063447;
RA Glaser P., Frangeul L., Buchrieser C., Rusniok C., Amend A., Baquero F.,
RA Berche P., Bloecker H., Brandt P., Chakraborty T., Charbit A.,
RA Chetouani F., Couve E., de Daruvar A., Dehoux P., Domann E.,
RA Dominguez-Bernal G., Duchaud E., Durant L., Dussurget O., Entian K.-D.,
RA Fsihi H., Garcia-del Portillo F., Garrido P., Gautier L., Goebel W.,
RA Gomez-Lopez N., Hain T., Hauf J., Jackson D., Jones L.-M., Kaerst U.,
RA Kreft J., Kuhn M., Kunst F., Kurapkat G., Madueno E., Maitournam A.,
RA Mata Vicente J., Ng E., Nedjari H., Nordsiek G., Novella S., de Pablos B.,
RA Perez-Diaz J.-C., Purcell R., Remmel B., Rose M., Schlueter T., Simoes N.,
RA Tierrez A., Vazquez-Boland J.-A., Voss H., Wehland J., Cossart P.;
RT "Comparative genomics of Listeria species.";
RL Science 294:849-852(2001).
RN [2]
RP FUNCTION AS A RIBOFLAVIN TRANSPORTER.
RX PubMed=27672192; DOI=10.1128/jb.00388-16;
RA Matern A., Pedrolli D., Grosshennig S., Johansson J., Mack M.;
RT "Uptake and metabolism of antibiotics roseoflavin and 8-demethyl-8-
RT aminoriboflavin in riboflavin-auxotrophic Listeria monocytogenes.";
RL J. Bacteriol. 198:3233-3243(2016).
RN [3]
RP FUNCTION AS A FMN/FAD TRANSPORTER, FUNCTION IN VIRULENCE, AND DISRUPTION
RP PHENOTYPE.
RC STRAIN=10403S;
RX PubMed=35316134; DOI=10.1073/pnas.2122173119;
RA Rivera-Lugo R., Light S.H., Garelis N.E., Portnoy D.A.;
RT "RibU is an essential determinant of Listeria pathogenesis that mediates
RT acquisition of FMN and FAD during intracellular growth.";
RL Proc. Natl. Acad. Sci. U.S.A. 119:e2122173119-e2122173119(2022).
CC -!- FUNCTION: Substrate-binding (S) component of an energy-coupling factor
CC (ECF) ABC-transporter complex (By similarity). Mediates uptake of
CC riboflavin (PubMed:27672192). Can also transport roseoflavin (RoF), and
CC probably 8-demethyl-8-aminoriboflavin (AF), toxic riboflavin analogs
CC (PubMed:27672192). In addition, during intracellular growth, binds and
CC imports FMN and FAD directly from the cytosol of host cells
CC (PubMed:35316134). Essential for virulence and intracellular growth
CC (PubMed:35316134). {ECO:0000250|UniProtKB:Q5M614,
CC ECO:0000269|PubMed:27672192, ECO:0000269|PubMed:35316134}.
CC -!- SUBUNIT: Forms a stable energy-coupling factor (ECF) transporter
CC complex composed of 2 membrane-embedded substrate-binding proteins (S
CC component), 2 ATP-binding proteins (A component) and 2 transmembrane
CC proteins (T component). {ECO:0000250|UniProtKB:Q5M614}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000255}.
CC -!- DISRUPTION PHENOTYPE: Mutant lacking this gene is completely avirulent
CC in mice but has no detectable growth defect in nutrient-rich media
CC (PubMed:35316134). Deletion mutant shows only a slight defect in growth
CC in media containing riboflavin, but does not replicate in chemically
CC defined media with FMN or FAD (PubMed:35316134).
CC {ECO:0000269|PubMed:35316134}.
CC -!- SIMILARITY: Belongs to the prokaryotic riboflavin transporter (P-RFT)
CC (TC 2.A.87) family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AL591981; CAD00023.1; -; Genomic_DNA.
DR PIR; AI1317; AI1317.
DR RefSeq; NP_465469.1; NC_003210.1.
DR RefSeq; WP_003732031.1; NZ_CP023861.1.
DR AlphaFoldDB; Q8Y5W0; -.
DR SMR; Q8Y5W0; -.
DR STRING; 169963.lmo1945; -.
DR TCDB; 2.A.87.1.4; the prokaryotic riboflavin transporter (p-rft) family.
DR TCDB; 3.A.1.25.8; the atp-binding cassette (abc) superfamily.
DR PaxDb; Q8Y5W0; -.
DR EnsemblBacteria; CAD00023; CAD00023; CAD00023.
DR GeneID; 984894; -.
DR KEGG; lmo:lmo1945; -.
DR PATRIC; fig|169963.11.peg.1992; -.
DR eggNOG; COG3601; Bacteria.
DR HOGENOM; CLU_086673_2_2_9; -.
DR OMA; MFLEFPI; -.
DR PhylomeDB; Q8Y5W0; -.
DR BioCyc; LMON169963:LMO1945-MON; -.
DR Proteomes; UP000000817; Chromosome.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0032217; F:riboflavin transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0032218; P:riboflavin transport; IBA:GO_Central.
DR InterPro; IPR024529; ECF_trnsprt_substrate-spec.
DR InterPro; IPR025720; RibU.
DR PANTHER; PTHR38438; PTHR38438; 1.
DR Pfam; PF12822; ECF_trnsprt; 1.
DR PIRSF; PIRSF037778; UCP037778_transp_RibU; 1.
PE 1: Evidence at protein level;
KW Cell membrane; Membrane; Reference proteome; Transmembrane;
KW Transmembrane helix; Transport; Virulence.
FT CHAIN 1..203
FT /note="Riboflavin transporter RibU"
FT /id="PRO_0000448895"
FT TRANSMEM 8..28
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 43..63
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 83..103
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 117..137
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 154..174
FT /note="Helical"
FT /evidence="ECO:0000255"
SQ SEQUENCE 203 AA; 22654 MW; EC9302D09D10BE5C CRC64;
MKNYSMKVFV SVAVLGTLAF ILMMLQFPLL PSAPFLKLDF SDIPALIGGL LFGPLAVILV
ELIKNVLLYI VSGSPVGVPV GELANFISGL FYVLPIYYLF HWLRSTKGMV LSTAVGTVLM
TGAMAVFNYF VLLPFYIKLG GLPANTDVAW LITYAIVPFN LLKGVIVSAV FLLLYSRLKK
WIAKNQTMKE RRKFEKRQQE ISH
