ID   RIBY_CHLAA              Reviewed;         351 AA.
AC   A9WGD2;
DT   02-NOV-2016, integrated into UniProtKB/Swiss-Prot.
DT   05-FEB-2008, sequence version 1.
DT   03-AUG-2022, entry version 63.
DE   RecName: Full=Riboflavin-binding protein RibY {ECO:0000305};
DE   Flags: Precursor;
GN   Name=ribY {ECO:0000303|PubMed:25345570, ECO:0000303|PubMed:25938806};
GN   OrderedLocusNames=Caur_0817 {ECO:0000312|EMBL:ABY34053.1};
OS   Chloroflexus aurantiacus (strain ATCC 29366 / DSM 635 / J-10-fl).
OC   Bacteria; Chloroflexi; Chloroflexia; Chloroflexales; Chloroflexineae;
OC   Chloroflexaceae; Chloroflexus.
OX   NCBI_TaxID=324602;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 29366 / DSM 635 / J-10-fl;
RX   PubMed=21714912; DOI=10.1186/1471-2164-12-334;
RA   Tang K.H., Barry K., Chertkov O., Dalin E., Han C.S., Hauser L.J.,
RA   Honchak B.M., Karbach L.E., Land M.L., Lapidus A., Larimer F.W.,
RA   Mikhailova N., Pitluck S., Pierson B.K., Blankenship R.E.;
RT   "Complete genome sequence of the filamentous anoxygenic phototrophic
RT   bacterium Chloroflexus aurantiacus.";
RL   BMC Genomics 12:334-334(2011).
RN   [2]
RP   SUBUNIT, AND RIBOFLAVIN BINDING.
RC   STRAIN=ATCC 29366 / DSM 635 / J-10-fl;
RX   PubMed=25345570; DOI=10.1111/1758-2229.12227;
RA   Rodionova I.A., Li X., Plymale A.E., Motamedchaboki K., Konopka A.E.,
RA   Romine M.F., Fredrickson J.K., Osterman A.L., Rodionov D.A.;
RT   "Genomic distribution of B-vitamin auxotrophy and uptake transporters in
RT   environmental bacteria from the Chloroflexi phylum.";
RL   Environ. Microbiol. Rep. 7:204-210(2015).
RN   [3]
RP   FUNCTION, AND INDUCTION.
RC   STRAIN=ATCC 29366 / DSM 635 / J-10-fl;
RX   PubMed=25938806; DOI=10.1371/journal.pone.0126124;
RA   Gutierrez-Preciado A., Torres A.G., Merino E., Bonomi H.R., Goldbaum F.A.,
RA   Garcia-Angulo V.A.;
RT   "Extensive identification of bacterial riboflavin transporters and their
RT   distribution across bacterial species.";
RL   PLoS ONE 10:E0126124-E0126124(2015).
CC   -!- FUNCTION: Part of an ABC transporter complex that transports riboflavin
CC       into the cell (PubMed:25938806). Binds riboflavin (PubMed:25345570).
CC       {ECO:0000269|PubMed:25345570, ECO:0000269|PubMed:25938806}.
CC   -!- SUBUNIT: The complex is likely composed of an ATP-binding protein, a
CC       transmembrane protein (RibX) and a solute-binding protein (RibY).
CC       {ECO:0000305|PubMed:25345570}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000255|PROSITE-
CC       ProRule:PRU00303}; Lipid-anchor {ECO:0000255|PROSITE-ProRule:PRU00303}.
CC   -!- INDUCTION: Expression is probably regulated by riboflavin, via an FMN
CC       riboswitch. {ECO:0000269|PubMed:25938806}.
CC   -!- SIMILARITY: Belongs to the NMT1 family. {ECO:0000305}.
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DR   EMBL; CP000909; ABY34053.1; -; Genomic_DNA.
DR   RefSeq; WP_012256709.1; NC_010175.1.
DR   RefSeq; YP_001634442.1; NC_010175.1.
DR   AlphaFoldDB; A9WGD2; -.
DR   SMR; A9WGD2; -.
DR   STRING; 324602.Caur_0817; -.
DR   TCDB; 3.A.1.17.14; the atp-binding cassette (abc) superfamily.
DR   EnsemblBacteria; ABY34053; ABY34053; Caur_0817.
DR   KEGG; cau:Caur_0817; -.
DR   PATRIC; fig|324602.8.peg.931; -.
DR   eggNOG; COG0715; Bacteria.
DR   HOGENOM; CLU_028871_1_0_0; -.
DR   InParanoid; A9WGD2; -.
DR   OMA; YVMAMYQ; -.
DR   Proteomes; UP000002008; Chromosome.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0009228; P:thiamine biosynthetic process; IBA:GO_Central.
DR   InterPro; IPR027939; NMT1/THI5.
DR   InterPro; IPR015168; SsuA/THI5.
DR   PANTHER; PTHR31528; PTHR31528; 1.
DR   Pfam; PF09084; NMT1; 1.
DR   PROSITE; PS51257; PROKAR_LIPOPROTEIN; 1.
PE   1: Evidence at protein level;
KW   Cell membrane; Lipoprotein; Membrane; Palmitate; Reference proteome;
KW   Signal.
FT   SIGNAL          1..19
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
FT   CHAIN           20..351
FT                   /note="Riboflavin-binding protein RibY"
FT                   /id="PRO_5002745825"
FT   LIPID           20
FT                   /note="N-palmitoyl cysteine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
FT   LIPID           20
FT                   /note="S-diacylglycerol cysteine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
SQ   SEQUENCE   351 AA;  37700 MW;  21441938532CE4A2 CRC64;
     MMKLRVLTLG ILIILLITAC SAPTPTTPAA APTAAPAPNA QPTLQQVTLA MSYIPNIQFA
     PYYVAAAKGY YAAEGIEVVF DYNFENDVLQ RAATWPTSGV AFATTSGTSV LLARQQGLPV
     KTVMTLYQRF PIAFFAKSNV PLASVNDLRG QTIGIPGRFG ESFYALLAAL YAGGMSEADV
     TVQEIGFTQT AAVMEDKVPV AIGYAMNEPV QLRGQGVEVN VLLAADVFNL AANGIAVSEA
     LIAQNPELVR KFVRASLRGL ADTLANPDEA FDLSLQFIPE AQLGDLSLQR QVLQESLPFW
     QNELTAQYGL GYTDGQLWTR TEEFMRAAGL LSAPVDVQQA FTNEFVPGGS Y