RIC8B_HUMAN
ID RIC8B_HUMAN Reviewed; 520 AA.
AC Q9NVN3; A2RTZ0; Q4G103; Q6ZRN4; Q86WD3;
DT 16-MAY-2006, integrated into UniProtKB/Swiss-Prot.
DT 05-OCT-2010, sequence version 2.
DT 03-AUG-2022, entry version 149.
DE RecName: Full=Synembryn-B;
DE AltName: Full=Brain synembryn;
DE Short=hSyn;
DE AltName: Full=Protein Ric-8B;
GN Name=RIC8B;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 4).
RC TISSUE=Teratocarcinoma, and Testis;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=16541075; DOI=10.1038/nature04569;
RA Scherer S.E., Muzny D.M., Buhay C.J., Chen R., Cree A., Ding Y.,
RA Dugan-Rocha S., Gill R., Gunaratne P., Harris R.A., Hawes A.C.,
RA Hernandez J., Hodgson A.V., Hume J., Jackson A., Khan Z.M., Kovar-Smith C.,
RA Lewis L.R., Lozado R.J., Metzker M.L., Milosavljevic A., Miner G.R.,
RA Montgomery K.T., Morgan M.B., Nazareth L.V., Scott G., Sodergren E.,
RA Song X.-Z., Steffen D., Lovering R.C., Wheeler D.A., Worley K.C., Yuan Y.,
RA Zhang Z., Adams C.Q., Ansari-Lari M.A., Ayele M., Brown M.J., Chen G.,
RA Chen Z., Clerc-Blankenburg K.P., Davis C., Delgado O., Dinh H.H.,
RA Draper H., Gonzalez-Garay M.L., Havlak P., Jackson L.R., Jacob L.S.,
RA Kelly S.H., Li L., Li Z., Liu J., Liu W., Lu J., Maheshwari M.,
RA Nguyen B.-V., Okwuonu G.O., Pasternak S., Perez L.M., Plopper F.J.H.,
RA Santibanez J., Shen H., Tabor P.E., Verduzco D., Waldron L., Wang Q.,
RA Williams G.A., Zhang J., Zhou J., Allen C.C., Amin A.G., Anyalebechi V.,
RA Bailey M., Barbaria J.A., Bimage K.E., Bryant N.P., Burch P.E.,
RA Burkett C.E., Burrell K.L., Calderon E., Cardenas V., Carter K., Casias K.,
RA Cavazos I., Cavazos S.R., Ceasar H., Chacko J., Chan S.N., Chavez D.,
RA Christopoulos C., Chu J., Cockrell R., Cox C.D., Dang M., Dathorne S.R.,
RA David R., Davis C.M., Davy-Carroll L., Deshazo D.R., Donlin J.E.,
RA D'Souza L., Eaves K.A., Egan A., Emery-Cohen A.J., Escotto M., Flagg N.,
RA Forbes L.D., Gabisi A.M., Garza M., Hamilton C., Henderson N.,
RA Hernandez O., Hines S., Hogues M.E., Huang M., Idlebird D.G., Johnson R.,
RA Jolivet A., Jones S., Kagan R., King L.M., Leal B., Lebow H., Lee S.,
RA LeVan J.M., Lewis L.C., London P., Lorensuhewa L.M., Loulseged H.,
RA Lovett D.A., Lucier A., Lucier R.L., Ma J., Madu R.C., Mapua P.,
RA Martindale A.D., Martinez E., Massey E., Mawhiney S., Meador M.G.,
RA Mendez S., Mercado C., Mercado I.C., Merritt C.E., Miner Z.L., Minja E.,
RA Mitchell T., Mohabbat F., Mohabbat K., Montgomery B., Moore N., Morris S.,
RA Munidasa M., Ngo R.N., Nguyen N.B., Nickerson E., Nwaokelemeh O.O.,
RA Nwokenkwo S., Obregon M., Oguh M., Oragunye N., Oviedo R.J., Parish B.J.,
RA Parker D.N., Parrish J., Parks K.L., Paul H.A., Payton B.A., Perez A.,
RA Perrin W., Pickens A., Primus E.L., Pu L.-L., Puazo M., Quiles M.M.,
RA Quiroz J.B., Rabata D., Reeves K., Ruiz S.J., Shao H., Sisson I.,
RA Sonaike T., Sorelle R.P., Sutton A.E., Svatek A.F., Svetz L.A.,
RA Tamerisa K.S., Taylor T.R., Teague B., Thomas N., Thorn R.D., Trejos Z.Y.,
RA Trevino B.K., Ukegbu O.N., Urban J.B., Vasquez L.I., Vera V.A.,
RA Villasana D.M., Wang L., Ward-Moore S., Warren J.T., Wei X., White F.,
RA Williamson A.L., Wleczyk R., Wooden H.S., Wooden S.H., Yen J., Yoon L.,
RA Yoon V., Zorrilla S.E., Nelson D., Kucherlapati R., Weinstock G.,
RA Gibbs R.A.;
RT "The finished DNA sequence of human chromosome 12.";
RL Nature 440:346-351(2006).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 3 AND 5).
RC TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 23-483 (ISOFORM 1), SUBCELLULAR LOCATION, AND
RP INTERACTION WITH GNAS AND GNAQ.
RC TISSUE=Brain;
RX PubMed=12652642; DOI=10.1002/jcp.10300;
RA Klattenhoff C., Montecino M., Soto X., Guzman L., Romo X., Garcia M.A.,
RA Mellstrom B., Naranjo J.R., Hinrichs M.V., Olate J.;
RT "Human brain synembryn interacts with Gsalpha and Gqalpha and is
RT translocated to the plasma membrane in response to isoproterenol and
RT carbachol.";
RL J. Cell. Physiol. 195:151-157(2003).
RN [5]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-468 AND THR-473, AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Leukemic T-cell;
RX PubMed=19690332; DOI=10.1126/scisignal.2000007;
RA Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K.,
RA Rodionov V., Han D.K.;
RT "Quantitative phosphoproteomic analysis of T cell receptor signaling
RT reveals system-wide modulation of protein-protein interactions.";
RL Sci. Signal. 2:RA46-RA46(2009).
CC -!- FUNCTION: Guanine nucleotide exchange factor (GEF), which can activate
CC some, but not all, G-alpha proteins by exchanging bound GDP for free
CC GTP. Able to potentiate G(olf)-alpha-dependent cAMP accumulation
CC suggesting that it may be an important component for odorant signal
CC transduction. {ECO:0000250}.
CC -!- SUBUNIT: Interacts with GDP-bound G alpha proteins GNAI1, GNAL, GNAS
CC and GNAQ. Does not interact with G-alpha proteins when they are in
CC complex with subunits beta and gamma. {ECO:0000269|PubMed:12652642}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cell cortex
CC {ECO:0000269|PubMed:12652642}. Note=Localizes to the cell cortex.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=4;
CC Name=5;
CC IsoId=Q9NVN3-5; Sequence=Displayed;
CC Name=1;
CC IsoId=Q9NVN3-1; Sequence=VSP_039866;
CC Name=3;
CC IsoId=Q9NVN3-3; Sequence=VSP_018511, VSP_039866;
CC Name=4;
CC IsoId=Q9NVN3-4; Sequence=VSP_018510, VSP_018512, VSP_018513;
CC -!- MISCELLANEOUS: [Isoform 1]: May be produced at very low levels due to a
CC premature stop codon in the mRNA, leading to nonsense-mediated mRNA
CC decay. {ECO:0000305}.
CC -!- MISCELLANEOUS: [Isoform 4]: May be produced at very low levels due to a
CC premature stop codon in the mRNA, leading to nonsense-mediated mRNA
CC decay. {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the synembryn family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAO65923.1; Type=Miscellaneous discrepancy; Note=Intron retention.; Evidence={ECO:0000305};
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DR EMBL; AK001482; BAA91717.1; -; mRNA.
DR EMBL; AK128102; -; NOT_ANNOTATED_CDS; mRNA.
DR EMBL; AC007695; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AC079385; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AC090011; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC034220; AAH34220.1; -; mRNA.
DR EMBL; BC132689; AAI32690.1; -; mRNA.
DR EMBL; BC144056; AAI44057.1; -; mRNA.
DR EMBL; AY221116; AAO65923.1; ALT_SEQ; mRNA.
DR CCDS; CCDS81733.1; -. [Q9NVN3-1]
DR CCDS; CCDS86331.1; -. [Q9NVN3-3]
DR CCDS; CCDS9109.2; -. [Q9NVN3-5]
DR RefSeq; NP_001317074.1; NM_001330145.1.
DR RefSeq; NP_001317075.1; NM_001330146.1.
DR RefSeq; NP_001317076.1; NM_001330147.1. [Q9NVN3-1]
DR RefSeq; NP_060627.2; NM_018157.3. [Q9NVN3-5]
DR AlphaFoldDB; Q9NVN3; -.
DR SMR; Q9NVN3; -.
DR BioGRID; 120486; 46.
DR IntAct; Q9NVN3; 10.
DR STRING; 9606.ENSP00000376583; -.
DR iPTMnet; Q9NVN3; -.
DR PhosphoSitePlus; Q9NVN3; -.
DR BioMuta; RIC8B; -.
DR DMDM; 308153488; -.
DR EPD; Q9NVN3; -.
DR jPOST; Q9NVN3; -.
DR MassIVE; Q9NVN3; -.
DR MaxQB; Q9NVN3; -.
DR PaxDb; Q9NVN3; -.
DR PeptideAtlas; Q9NVN3; -.
DR PRIDE; Q9NVN3; -.
DR ProteomicsDB; 82832; -. [Q9NVN3-5]
DR ProteomicsDB; 82833; -. [Q9NVN3-1]
DR ProteomicsDB; 82834; -. [Q9NVN3-3]
DR ProteomicsDB; 82835; -. [Q9NVN3-4]
DR Antibodypedia; 45122; 61 antibodies from 17 providers.
DR DNASU; 55188; -.
DR Ensembl; ENST00000355478.6; ENSP00000347662.2; ENSG00000111785.21. [Q9NVN3-3]
DR Ensembl; ENST00000392839.6; ENSP00000376583.2; ENSG00000111785.21. [Q9NVN3-5]
DR Ensembl; ENST00000462949.5; ENSP00000435301.1; ENSG00000111785.21. [Q9NVN3-1]
DR Ensembl; ENST00000470960.1; ENSP00000436359.1; ENSG00000111785.21. [Q9NVN3-4]
DR GeneID; 55188; -.
DR KEGG; hsa:55188; -.
DR UCSC; uc001tlx.4; human. [Q9NVN3-5]
DR CTD; 55188; -.
DR DisGeNET; 55188; -.
DR GeneCards; RIC8B; -.
DR HGNC; HGNC:25555; RIC8B.
DR HPA; ENSG00000111785; Low tissue specificity.
DR MIM; 609147; gene.
DR neXtProt; NX_Q9NVN3; -.
DR OpenTargets; ENSG00000111785; -.
DR PharmGKB; PA142671064; -.
DR VEuPathDB; HostDB:ENSG00000111785; -.
DR eggNOG; KOG4464; Eukaryota.
DR GeneTree; ENSGT00390000014700; -.
DR HOGENOM; CLU_018602_1_0_1; -.
DR InParanoid; Q9NVN3; -.
DR OrthoDB; 1110075at2759; -.
DR PhylomeDB; Q9NVN3; -.
DR TreeFam; TF314907; -.
DR PathwayCommons; Q9NVN3; -.
DR SignaLink; Q9NVN3; -.
DR BioGRID-ORCS; 55188; 5 hits in 1076 CRISPR screens.
DR ChiTaRS; RIC8B; human.
DR GenomeRNAi; 55188; -.
DR Pharos; Q9NVN3; Tbio.
DR PRO; PR:Q9NVN3; -.
DR Proteomes; UP000005640; Chromosome 12.
DR RNAct; Q9NVN3; protein.
DR Bgee; ENSG00000111785; Expressed in right uterine tube and 180 other tissues.
DR ExpressionAtlas; Q9NVN3; baseline and differential.
DR Genevisible; Q9NVN3; HS.
DR GO; GO:0005938; C:cell cortex; IEA:UniProtKB-SubCell.
DR GO; GO:0005813; C:centrosome; IDA:HPA.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0005829; C:cytosol; IDA:HPA.
DR GO; GO:0005886; C:plasma membrane; IDA:HPA.
DR GO; GO:0001965; F:G-protein alpha-subunit binding; IDA:MGI.
DR GO; GO:0005085; F:guanyl-nucleotide exchange factor activity; IBA:GO_Central.
DR GO; GO:0007186; P:G protein-coupled receptor signaling pathway; IBA:GO_Central.
DR GO; GO:0008277; P:regulation of G protein-coupled receptor signaling pathway; IDA:MGI.
DR Gene3D; 1.25.10.10; -; 1.
DR InterPro; IPR011989; ARM-like.
DR InterPro; IPR016024; ARM-type_fold.
DR InterPro; IPR019318; Gua_nucleotide_exch_fac_Ric8.
DR InterPro; IPR008376; Synembryn.
DR PANTHER; PTHR12425; PTHR12425; 2.
DR Pfam; PF10165; Ric8; 1.
DR PRINTS; PR01802; SYNEMBRYN.
DR SUPFAM; SSF48371; SSF48371; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Cytoplasm; Guanine-nucleotide releasing factor;
KW Phosphoprotein; Reference proteome.
FT CHAIN 1..520
FT /note="Synembryn-B"
FT /id="PRO_0000235899"
FT REGION 468..494
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 475..491
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 468
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:19690332"
FT MOD_RES 473
FT /note="Phosphothreonine"
FT /evidence="ECO:0007744|PubMed:19690332"
FT VAR_SEQ 1..240
FT /note="Missing (in isoform 4)"
FT /evidence="ECO:0000303|PubMed:14702039"
FT /id="VSP_018510"
FT VAR_SEQ 1..44
FT /note="MDEERALYIVRAGEAGAIERVLRDYSDKHRATFKFESTDEDKRK -> MLAS
FT (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_018511"
FT VAR_SEQ 241..247
FT /note="SWKVHKE -> MLSKLEK (in isoform 4)"
FT /evidence="ECO:0000303|PubMed:14702039"
FT /id="VSP_018512"
FT VAR_SEQ 484..520
FT /note="KEELLKPMGLKPDGTITPLEEALNQYSVIEETSSDTD -> NINLITGHLEE
FT PMPNPIDEMTEEQKEYEAMKLVNMLDKLSRRADVKDLHQDGG (in isoform 1
FT and isoform 3)"
FT /evidence="ECO:0000303|PubMed:12652642,
FT ECO:0000303|PubMed:14702039, ECO:0000303|PubMed:15489334"
FT /id="VSP_039866"
FT VAR_SEQ 484..520
FT /note="KEELLKPMGLKPDGTITPLEEALNQYSVIEETSSDTD -> NSIEFEERSHL
FT SRGK (in isoform 4)"
FT /evidence="ECO:0000303|PubMed:14702039"
FT /id="VSP_018513"
FT CONFLICT 201
FT /note="S -> T (in Ref. 3; AAO65923)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 520 AA; 58825 MW; C4D6B01725E6FB22 CRC64;
MDEERALYIV RAGEAGAIER VLRDYSDKHR ATFKFESTDE DKRKKLCEGI FKVLIKDIPT
TCQVSCLEVL RILSRDKKVL VPVTTKENMQ ILLRLAKLNE LDDSLEKVSE FPVIVESLKC
LCNIVFNSQM AQQLSLELNL AAKLCNLLRK CKDRKFINDI KCFDLRLLFL LSLLHTDIRS
QLRYELQGLP LLTQILESAF SIKWTDEYES AIDHNGPPLS PQETDCAIEA LKALFNVTVD
SWKVHKESDS HQFRVMAAVL RHCLLIVGPT EDKTEELHSN AVNLLSNVPV SCLDVLICPL
THEETAQEAT TLDELPSNKT AEKETVLKNN TMVYNGMNME AIHVLLNFME KRIDKGSSYR
EGLTPVLSLL TECSRAHRNI RKFLKDQVLP PLRDVTNRPE VGSTVRNKLV RLMTHVDLGV
KQIAAEFLFV LCKERVDSLL KYTGYGNAAG LLAARGLLAG GRGDNWYSED EDTDTEEYKN
AKPKEELLKP MGLKPDGTIT PLEEALNQYS VIEETSSDTD
