ID   RIC8B_RAT               Reviewed;         520 AA.
AC   Q80ZG0;
DT   16-MAY-2006, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2003, sequence version 1.
DT   03-AUG-2022, entry version 93.
DE   RecName: Full=Synembryn-B;
DE   AltName: Full=Protein Ric-8B;
GN   Name=Ric8b;
OS   Rattus norvegicus (Rat).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Rattus.
OX   NCBI_TaxID=10116;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], AND INTERACTION WITH GNAI1; GNAS AND GNAQ.
RC   STRAIN=Sprague-Dawley;
RX   PubMed=12509430; DOI=10.1074/jbc.m211862200;
RA   Tall G.G., Krumins A.M., Gilman A.G.;
RT   "Mammalian Ric-8A (synembryn) is a heterotrimeric Galpha protein guanine
RT   nucleotide exchange factor.";
RL   J. Biol. Chem. 278:8356-8362(2003).
RN   [2]
RP   PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-468 AND THR-473, AND
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX   PubMed=22673903; DOI=10.1038/ncomms1871;
RA   Lundby A., Secher A., Lage K., Nordsborg N.B., Dmytriyev A., Lundby C.,
RA   Olsen J.V.;
RT   "Quantitative maps of protein phosphorylation sites across 14 different rat
RT   organs and tissues.";
RL   Nat. Commun. 3:876-876(2012).
CC   -!- FUNCTION: Guanine nucleotide exchange factor (GEF), which can activate
CC       some, but not all, G-alpha proteins by exchanging bound GDP for free
CC       GTP. Able to potentiate G(olf)-alpha-dependent cAMP accumulation
CC       suggesting that it may be an important component for odorant signal
CC       transduction (Probable). {ECO:0000305}.
CC   -!- SUBUNIT: Interacts with GDP-bound G alpha proteins GNAI1, GNAL, GNAS
CC       and GNAQ. Does not interact with G-alpha proteins when they are in
CC       complex with subunits beta and gamma. {ECO:0000269|PubMed:12509430}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm, cell cortex {ECO:0000250}.
CC       Note=Localizes to the cell cortex. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the synembryn family. {ECO:0000305}.
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DR   EMBL; AY177755; AAO23337.1; -; mRNA.
DR   RefSeq; NP_783188.1; NM_175598.2.
DR   AlphaFoldDB; Q80ZG0; -.
DR   SMR; Q80ZG0; -.
DR   STRING; 10116.ENSRNOP00000009625; -.
DR   iPTMnet; Q80ZG0; -.
DR   PhosphoSitePlus; Q80ZG0; -.
DR   PaxDb; Q80ZG0; -.
DR   PRIDE; Q80ZG0; -.
DR   Ensembl; ENSRNOT00000009625; ENSRNOP00000009625; ENSRNOG00000007323.
DR   GeneID; 314681; -.
DR   KEGG; rno:314681; -.
DR   UCSC; RGD:631419; rat.
DR   CTD; 55188; -.
DR   RGD; 631419; Ric8b.
DR   eggNOG; KOG4464; Eukaryota.
DR   GeneTree; ENSGT00390000014700; -.
DR   InParanoid; Q80ZG0; -.
DR   OrthoDB; 1110075at2759; -.
DR   PRO; PR:Q80ZG0; -.
DR   Proteomes; UP000002494; Chromosome 7.
DR   Bgee; ENSRNOG00000007323; Expressed in quadriceps femoris and 18 other tissues.
DR   ExpressionAtlas; Q80ZG0; baseline and differential.
DR   GO; GO:0005938; C:cell cortex; IEA:UniProtKB-SubCell.
DR   GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR   GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR   GO; GO:0001965; F:G-protein alpha-subunit binding; ISO:RGD.
DR   GO; GO:0005085; F:guanyl-nucleotide exchange factor activity; IBA:GO_Central.
DR   GO; GO:0007186; P:G protein-coupled receptor signaling pathway; IBA:GO_Central.
DR   GO; GO:0008277; P:regulation of G protein-coupled receptor signaling pathway; ISO:RGD.
DR   Gene3D; 1.25.10.10; -; 1.
DR   InterPro; IPR011989; ARM-like.
DR   InterPro; IPR016024; ARM-type_fold.
DR   InterPro; IPR019318; Gua_nucleotide_exch_fac_Ric8.
DR   InterPro; IPR008376; Synembryn.
DR   PANTHER; PTHR12425; PTHR12425; 2.
DR   Pfam; PF10165; Ric8; 1.
DR   PRINTS; PR01802; SYNEMBRYN.
DR   SUPFAM; SSF48371; SSF48371; 1.
PE   1: Evidence at protein level;
KW   Cytoplasm; Guanine-nucleotide releasing factor; Phosphoprotein;
KW   Reference proteome.
FT   CHAIN           1..520
FT                   /note="Synembryn-B"
FT                   /id="PRO_0000235901"
FT   MOD_RES         468
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0007744|PubMed:22673903"
FT   MOD_RES         473
FT                   /note="Phosphothreonine"
FT                   /evidence="ECO:0007744|PubMed:22673903"
SQ   SEQUENCE   520 AA;  58390 MW;  9A0166AD61385DA0 CRC64;
     MDEERALYIV RAGEAGAIER VLRDYSDKHR ATFKFESADE DKRKKLCEGI FKVLVKGVPT
     TCQVPCLEVL RILSRDKKIL APVTTEENMQ ILLRLAKLHE SDDSLEKVSE FPVIVESLKC
     LCNIVFNSQV AQQLSLELNL AAKLCNLLRK CKDRKFINDI KCFDLRLLFV LSLLHTDIRS
     QLRYELQGLP LLTQILESAF SIKWTDEYES AIDHSGPPLS PQETDCAIEA LKALFNVTVD
     SWKVHKESDS HQFRVMAAVL RHCLLIVGPT EDKTEELHSN AVNLLSNVPV SCLDVLICPL
     THEETAQEAA TLDELPGDKT AEKDTALKSS AMVYNGMNME AIHVLLSFME KRIDKGSSYR
     EGLTPVLSLL TECSRAHRNI RKFLKDQVLP PLRDVTNRPE VGSTVRNKLV RLMTHVDLGV
     KQIAAEFLFV LCKERVDSLL KYTGYGNAAG LLAARGLLAG GRGDNWYSED EDTDTEEYKN
     AKPKEELLKP MGLKPDGTIT PLEEALSQYS VIEETSSDTD