ID   RIC8_DROPS              Reviewed;         566 AA.
AC   Q29IC2;
DT   16-MAY-2006, integrated into UniProtKB/Swiss-Prot.
DT   04-APR-2006, sequence version 1.
DT   03-AUG-2022, entry version 81.
DE   RecName: Full=Synembryn;
DE   AltName: Full=Protein Ric-8;
GN   Name=ric8a; Synonyms=Ric8; ORFNames=GA13961;
OS   Drosophila pseudoobscura pseudoobscura (Fruit fly).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC   Drosophilidae; Drosophila; Sophophora.
OX   NCBI_TaxID=46245;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=MV2-25 / Tucson 14011-0121.94;
RX   PubMed=15632085; DOI=10.1101/gr.3059305;
RA   Richards S., Liu Y., Bettencourt B.R., Hradecky P., Letovsky S.,
RA   Nielsen R., Thornton K., Hubisz M.J., Chen R., Meisel R.P., Couronne O.,
RA   Hua S., Smith M.A., Zhang P., Liu J., Bussemaker H.J., van Batenburg M.F.,
RA   Howells S.L., Scherer S.E., Sodergren E., Matthews B.B., Crosby M.A.,
RA   Schroeder A.J., Ortiz-Barrientos D., Rives C.M., Metzker M.L., Muzny D.M.,
RA   Scott G., Steffen D., Wheeler D.A., Worley K.C., Havlak P., Durbin K.J.,
RA   Egan A., Gill R., Hume J., Morgan M.B., Miner G., Hamilton C., Huang Y.,
RA   Waldron L., Verduzco D., Clerc-Blankenburg K.P., Dubchak I., Noor M.A.F.,
RA   Anderson W., White K.P., Clark A.G., Schaeffer S.W., Gelbart W.M.,
RA   Weinstock G.M., Gibbs R.A.;
RT   "Comparative genome sequencing of Drosophila pseudoobscura: chromosomal,
RT   gene, and cis-element evolution.";
RL   Genome Res. 15:1-18(2005).
CC   -!- FUNCTION: Guanine nucleotide exchange factor (GEF), which can activate
CC       some, but not all, G-alpha proteins independently of G-protein coupled
CC       receptors. Acts by exchanging bound GDP for free GTP. Plays a key role
CC       in asymmetric spindle positioning, a step for asymmetric cell division
CC       that generates cell diversity during development by activating G(i)
CC       alpha protein independently of G-protein coupled receptors. In addition
CC       to its GEF activity, it plays an essential role in cortical subcellular
CC       localization of heterotrimeric G proteins, suggesting it acts as a
CC       facilitator of G-alpha function through control of its membrane
CC       targeting and/or assembling of associated components rather than a GEF
CC       (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Interacts with GDP-bound G(i)-alpha protein. Does not interact
CC       with G-alpha proteins when they are in complex with subunits beta and
CC       gamma.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm, cell cortex. Note=Localizes to the
CC       cell cortex.
CC   -!- SIMILARITY: Belongs to the synembryn family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; CH379063; EAL32731.1; -; Genomic_DNA.
DR   RefSeq; XP_001355672.1; XM_001355636.3.
DR   AlphaFoldDB; Q29IC2; -.
DR   SMR; Q29IC2; -.
DR   STRING; 7237.FBpp0272666; -.
DR   EnsemblMetazoa; FBtr0274228; FBpp0272666; FBgn0073993.
DR   GeneID; 4816097; -.
DR   KEGG; dpo:Dpse_GA13961; -.
DR   eggNOG; KOG4464; Eukaryota.
DR   HOGENOM; CLU_018602_1_0_1; -.
DR   InParanoid; Q29IC2; -.
DR   OMA; NADPIFT; -.
DR   PhylomeDB; Q29IC2; -.
DR   Proteomes; UP000001819; Chromosome X.
DR   Bgee; FBgn0073993; Expressed in female reproductive system and 2 other tissues.
DR   GO; GO:0005938; C:cell cortex; IEA:UniProtKB-SubCell.
DR   GO; GO:0005737; C:cytoplasm; ISS:UniProtKB.
DR   GO; GO:0005829; C:cytosol; IEA:EnsemblMetazoa.
DR   GO; GO:0001965; F:G-protein alpha-subunit binding; ISS:UniProtKB.
DR   GO; GO:0005085; F:guanyl-nucleotide exchange factor activity; ISS:UniProtKB.
DR   GO; GO:0008356; P:asymmetric cell division; ISS:UniProtKB.
DR   GO; GO:0040001; P:establishment of mitotic spindle localization; ISS:UniProtKB.
DR   GO; GO:0007163; P:establishment or maintenance of cell polarity; ISS:UniProtKB.
DR   GO; GO:0010004; P:gastrulation involving germ band extension; ISS:UniProtKB.
DR   GO; GO:0055057; P:neuroblast division; ISS:UniProtKB.
DR   GO; GO:0008104; P:protein localization; ISS:UniProtKB.
DR   GO; GO:0050821; P:protein stabilization; ISS:UniProtKB.
DR   Gene3D; 1.25.10.10; -; 1.
DR   InterPro; IPR011989; ARM-like.
DR   InterPro; IPR016024; ARM-type_fold.
DR   InterPro; IPR019318; Gua_nucleotide_exch_fac_Ric8.
DR   InterPro; IPR008376; Synembryn.
DR   PANTHER; PTHR12425; PTHR12425; 1.
DR   Pfam; PF10165; Ric8; 1.
DR   PRINTS; PR01802; SYNEMBRYN.
DR   SUPFAM; SSF48371; SSF48371; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; Developmental protein; Guanine-nucleotide releasing factor;
KW   Reference proteome.
FT   CHAIN           1..566
FT                   /note="Synembryn"
FT                   /id="PRO_0000235906"
SQ   SEQUENCE   566 AA;  64450 MW;  AAED9E18F0E9008C CRC64;
     MDAEHLKRLE GREAEHIPDI LNEFNKKHAD LLVFDSFHSD NQWHELWLAI FGILDDAQLG
     HLHTQCLNTV RILTRDEFSL QTNYIEHEVS QLLRLARVEA SSLKLPATPD ELKQQEEGQL
     QLQEQPSLAQ SDIIAEALKC LCNLVYQSAD CRRQCLRQHC LDAILKRVAS SMRHPCALEY
     YDMKLLFLLT ALEPAARTRL QIDLNGLTYM TKWLDDKLAE PSCSEEQLNI ICELLKVMFN
     VTSAPDKSPN EYEIQSLHLT GVLRELLLRF GKMATDKERS VVTHAINLLT NISSSCLIEL
     TLKCSNAEEQ PKKAVEGVAK AKPAKCCALC FEKRNVRCLT VLLNYLRQAL AQQEAEASSH
     ELLSPVLTVL VKCSRSDRVM RHYLRQEILP PLRDVSQRPE IGQELRNHLC RFLTLPAMIL
     RDLAAELLFV LCKEDVGRMI KYTGYGNAAG LFAKRGILDC RRVEGADYSS DSEDSDTEEY
     KKHQQGINPV LGCVEPKSKH NPLDDMTEEQ KESEALQLVN LIEQLRQGGI VKPALIDKDG
     KPQPLEHILQ LQEELPQQQL EQKRKT