RIF1_DROME
ID RIF1_DROME Reviewed; 1416 AA.
AC Q9XZ34; Q4V5E2;
DT 18-JUL-2018, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1999, sequence version 1.
DT 03-AUG-2022, entry version 155.
DE RecName: Full=Telomere-associated protein RIF1 {ECO:0000305};
DE AltName: Full=Rap1 interacting factor 1 {ECO:0000303|PubMed:22712556, ECO:0000312|FlyBase:FBgn0050085};
GN Name=Rif1 {ECO:0000303|PubMed:22712556, ECO:0000312|FlyBase:FBgn0050085};
GN ORFNames=CG30085 {ECO:0000312|FlyBase:FBgn0050085};
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227 {ECO:0000312|Proteomes:UP000000803};
RN [1] {ECO:0000312|Proteomes:UP000000803}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley {ECO:0000312|Proteomes:UP000000803};
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2] {ECO:0000312|Proteomes:UP000000803}
RP GENOME REANNOTATION.
RC STRAIN=Berkeley {ECO:0000312|Proteomes:UP000000803};
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3] {ECO:0000312|EMBL:AAD34780.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley {ECO:0000312|EMBL:AAD34780.1};
RC TISSUE=Embryo {ECO:0000312|EMBL:AAD34780.1};
RX PubMed=10731138; DOI=10.1126/science.287.5461.2222;
RA Rubin G.M., Hong L., Brokstein P., Evans-Holm M., Frise E., Stapleton M.,
RA Harvey D.A.;
RT "A Drosophila complementary DNA resource.";
RL Science 287:2222-2224(2000).
RN [4] {ECO:0000312|EMBL:AAY55130.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley {ECO:0000312|EMBL:AAY55130.1};
RC TISSUE=Embryo {ECO:0000312|EMBL:AAY55130.1};
RA Stapleton M., Carlson J., Chavez C., Frise E., George R., Pacleb J.,
RA Park S., Wan K., Yu C., Celniker S.;
RL Submitted (MAY-2005) to the EMBL/GenBank/DDBJ databases.
RN [5] {ECO:0000305}
RP FUNCTION, SUBCELLULAR LOCATION, AND DEVELOPMENTAL STAGE.
RX PubMed=22712556; DOI=10.1186/1471-2164-13-255;
RA Sreesankar E., Senthilkumar R., Bharathi V., Mishra R.K., Mishra K.;
RT "Functional diversification of yeast telomere associated protein, Rif1, in
RT higher eukaryotes.";
RL BMC Genomics 13:255-255(2012).
RN [6] {ECO:0000305}
RP FUNCTION, INTERACTION WITH PP1-87B, SUBCELLULAR LOCATION, TISSUE
RP SPECIFICITY, DEVELOPMENTAL STAGE, AND DISRUPTION PHENOTYPE.
RX PubMed=26022086; DOI=10.1038/srep10679;
RA Sreesankar E., Bharathi V., Mishra R.K., Mishra K.;
RT "Drosophila Rif1 is an essential gene and controls late developmental
RT events by direct interaction with PP1-87B.";
RL Sci. Rep. 5:10679-10679(2015).
RN [7]
RP ERRATUM OF PUBMED:26022086.
RX PubMed=26859551; DOI=10.1038/srep17126;
RA Sreesankar E., Bharathi V., Mishra R.K., Mishra K.;
RT "Corrigendum: Drosophila Rif1 is an essential gene and controls late
RT developmental events by direct interaction with PP1-87B.";
RL Sci. Rep. 6:17126-17126(2016).
RN [8]
RP FUNCTION, INTERACTION WITH SUUR, SUBCELLULAR LOCATION, TISSUE SPECIFICITY,
RP DISRUPTION PHENOTYPE, AND MUTAGENESIS OF 1076-ILE-LEU-1077 AND
RP 1101-VAL--PHE-1103.
RX PubMed=30277458; DOI=10.7554/elife.39140;
RA Munden A., Rong Z., Sun A., Gangula R., Mallal S., Nordman J.T.;
RT "Rif1 inhibits replication fork progression and controls DNA copy number in
RT Drosophila.";
RL Elife 7:0-0(2018).
RN [9] {ECO:0000305}
RP FUNCTION, INTERACTION WITH PP1-87B, SUBCELLULAR LOCATION, DEVELOPMENTAL
RP STAGE, DISRUPTION PHENOTYPE, AND MUTAGENESIS OF SER-946; 958-SER-SER-959;
RP 969-SER-SER-970; SER-1002; THR-1019; 1031-SER-THR-1032; SER-1035; THR-1043;
RP 1047-SER-THR-1048; SER-1067; SER-1071; SER-1075; SER-1093; SER-1102 AND
RP THR-1140.
RX PubMed=29746464; DOI=10.1371/journal.pbio.2005687;
RA Seller C.A., O'Farrell P.H.;
RT "Rif1 prolongs the embryonic S phase at the Drosophila mid-blastula
RT transition.";
RL PLoS Biol. 16:E2005687-E2005687(2018).
CC -!- FUNCTION: Regulates the timing of initiation of DNA replication
CC (PubMed:29746464, PubMed:30277458, PubMed:26022086). Functions in copy
CC number control by promoting the underreplication of DNA, which is found
CC in many late replicating euchromatic regions of salivary gland polytene
CC chromosomes (PubMed:30277458). Promotes underreplication by localizing
CC to active DNA replication forks in a partially SuUR-dependent manner,
CC and inhibiting replication fork progression (PubMed:30277458). Might
CC also work as an adapter to recruit Pp1-87B to multiple sites on the
CC chromosome and may function with Pp1-87B to mediate underreplication
CC (PubMed:26022086, PubMed:29746464, PubMed:30277458). Plays an essential
CC role in embryonic development, in the transition from larvae to pupae
CC and, probably, in proliferating tissues later on (PubMed:26022086,
CC PubMed:29746464). In embryos, during mid-blastula transition, binds to
CC and selectively delays the replication of large blocks of repetitive
CC DNA satellite sequences during S phase in response to the activity of
CC Cdk1; maternal Rif1 is specifically required for the normal extension
CC of S phase 14 (PubMed:29746464). Unlike mammalian orthologs, does not
CC appear to play a role in DNA damage repair (PubMed:22712556,
CC PubMed:26022086). {ECO:0000269|PubMed:22712556,
CC ECO:0000269|PubMed:26022086, ECO:0000269|PubMed:29746464,
CC ECO:0000269|PubMed:30277458}.
CC -!- SUBUNIT: Interacts with Pp1-87b (PubMed:26022086, PubMed:29746464).
CC Interacts with SuUR (via SNF2-like region) (PubMed:30277458).
CC {ECO:0000269|PubMed:26022086, ECO:0000269|PubMed:29746464,
CC ECO:0000269|PubMed:30277458}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:22712556,
CC ECO:0000269|PubMed:26022086, ECO:0000269|PubMed:29746464,
CC ECO:0000269|PubMed:30277458}. Chromosome {ECO:0000269|PubMed:22712556,
CC ECO:0000269|PubMed:26022086, ECO:0000269|PubMed:29746464,
CC ECO:0000269|PubMed:30277458}. Chromosome, telomere
CC {ECO:0000269|PubMed:26022086}. Note=Localizes to heterochromatin in all
CC stages of amplifying follicle cells and accumulates at active DNA
CC replication forks in the later stages of DNA amplification
CC (PubMed:30277458). Associates with polytene chromosomes in salivary
CC glands (PubMed:26022086). Associates with heterochromatin in a dynamic
CC manner in different mitotic stages of the cell cycle (PubMed:22712556,
CC PubMed:26022086, PubMed:29746464, PubMed:30277458). Absent at metaphase
CC chromosomes but re-associates with the condensed chromosomes during
CC late anaphase (PubMed:22712556, PubMed:26022086, PubMed:29746464).
CC During mid-blastula transition in the S phase of embryos, dissociates
CC from chromatin before the replication of the bound DNA satellite
CC sequences (PubMed:29746464). {ECO:0000269|PubMed:22712556,
CC ECO:0000269|PubMed:26022086, ECO:0000269|PubMed:29746464,
CC ECO:0000269|PubMed:30277458}.
CC -!- TISSUE SPECIFICITY: Expressed in nurse cells and follicle cells in the
CC adult female (at protein level) (PubMed:26022086, PubMed:30277458).
CC Detected in adult at extremely low levels (PubMed:26022086).
CC {ECO:0000269|PubMed:26022086, ECO:0000269|PubMed:30277458}.
CC -!- DEVELOPMENTAL STAGE: Expressed in embryos at high levels between 0-2
CC hours; from 8 to 14 hours, the highest levels are in the brain then
CC diminishes to negligible levels (at protein level) (PubMed:22712556,
CC PubMed:26022086, PubMed:29746464). Expressed in larval imaginal disks
CC and salivary glands (at protein level) (PubMed:26022086). Detected in
CC larvae at lower levels in imaginal disks, the central nervous system
CC and ovary and, at extremely low levels, in testis (PubMed:26022086).
CC {ECO:0000269|PubMed:22712556, ECO:0000269|PubMed:26022086,
CC ECO:0000269|PubMed:29746464}.
CC -!- PTM: Phosphorylated, probably by Cdk1; phosphorylation regulates
CC dissociation from heterochromatin. {ECO:0000269|PubMed:29746464}.
CC -!- DISRUPTION PHENOTYPE: Embryos have a reduced hatch rate and develop
CC into adults with reduced survival (PubMed:29746464). During mid-
CC blastula transition in embryos, S phase 14 is significantly shorter
CC (PubMed:29746464). In larval salivary glands, loss of DNA
CC underreplication resulting in abnormally enlarged chromocenters
CC (PubMed:30277458). RNAi-mediated knockdown results in lethality
CC (PubMed:26022086). Escaper flies only live for a few days and are
CC infertile (PubMed:26022086). Females show increased necrotic spots in
CC the abdominal segments (PubMed:26022086). RNAi-mediated knockdown in
CC the imaginal disks does not affect viability (PubMed:26022086).
CC {ECO:0000269|PubMed:26022086, ECO:0000269|PubMed:29746464,
CC ECO:0000269|PubMed:30277458}.
CC -!- SIMILARITY: Belongs to the RIF1 family. Highly divergent.
CC {ECO:0000305}.
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DR EMBL; AE013599; AAM70965.1; -; Genomic_DNA.
DR EMBL; AF132192; AAD34780.1; -; mRNA.
DR EMBL; BT022714; AAY55130.1; -; mRNA.
DR RefSeq; NP_725497.1; NM_166120.2.
DR AlphaFoldDB; Q9XZ34; -.
DR STRING; 7227.FBpp0086441; -.
DR PaxDb; Q9XZ34; -.
DR PRIDE; Q9XZ34; -.
DR EnsemblMetazoa; FBtr0087306; FBpp0086441; FBgn0050085.
DR GeneID; 246445; -.
DR KEGG; dme:Dmel_CG30085; -.
DR UCSC; CG30085-RA; d. melanogaster.
DR CTD; 55183; -.
DR FlyBase; FBgn0050085; Rif1.
DR VEuPathDB; VectorBase:FBgn0050085; -.
DR eggNOG; ENOG502QV6C; Eukaryota.
DR GeneTree; ENSGT00390000012204; -.
DR HOGENOM; CLU_005092_0_0_1; -.
DR InParanoid; Q9XZ34; -.
DR OMA; PCLVALW; -.
DR OrthoDB; 70741at2759; -.
DR PhylomeDB; Q9XZ34; -.
DR BioGRID-ORCS; 246445; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 246445; -.
DR PRO; PR:Q9XZ34; -.
DR Proteomes; UP000000803; Chromosome 2R.
DR Bgee; FBgn0050085; Expressed in egg cell and 41 other tissues.
DR GO; GO:0010369; C:chromocenter; IDA:FlyBase.
DR GO; GO:0000781; C:chromosome, telomeric region; IDA:FlyBase.
DR GO; GO:0000792; C:heterochromatin; IDA:UniProtKB.
DR GO; GO:0043596; C:nuclear replication fork; IDA:FlyBase.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0007049; P:cell cycle; IEA:UniProtKB-KW.
DR GO; GO:0001700; P:embryonic development via the syncytial blastoderm; IMP:UniProtKB.
DR GO; GO:2000104; P:negative regulation of DNA-templated DNA replication; IMP:FlyBase.
DR GO; GO:1901991; P:negative regulation of mitotic cell cycle phase transition; IMP:UniProtKB.
DR GO; GO:0030174; P:regulation of DNA-templated DNA replication initiation; IMP:UniProtKB.
DR GO; GO:0071807; P:replication fork arrest involved in DNA replication termination; IMP:UniProtKB.
DR GO; GO:0000723; P:telomere maintenance; IBA:GO_Central.
DR InterPro; IPR016024; ARM-type_fold.
DR InterPro; IPR028566; Rif1.
DR InterPro; IPR022031; Rif1_N.
DR PANTHER; PTHR22928; PTHR22928; 1.
DR Pfam; PF12231; Rif1_N; 1.
DR SUPFAM; SSF48371; SSF48371; 1.
PE 1: Evidence at protein level;
KW Cell cycle; Chromosome; Nucleus; Reference proteome; Telomere.
FT CHAIN 1..1416
FT /note="Telomere-associated protein RIF1"
FT /id="PRO_0000444898"
FT REGION 789..858
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 886..984
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1021..1054
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1166..1186
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 789..803
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 951..977
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1021..1053
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MUTAGEN 946
FT /note="S->A: Probable loss of Cdk1-mediated regulation
FT leads to lack of dissociation from chromatin and block of
FT replication resulting in anaphase chromosome bridging
FT during mid-blastula transition in embryos; in the eye
FT imaginal disk, results in complete lethality with pupae
FT developing into headless nearly adult flies; when
FT associated with 958-A-A-959; 969-A-A-970; A-1002; A-1019;
FT 1031-A-A-1032; A-1035; A-1043; 1047-A-A-1048; A-1067; A-
FT 1071; A-1075; A-1093; A-1102 and A-1140."
FT /evidence="ECO:0000269|PubMed:29746464"
FT MUTAGEN 958..959
FT /note="SS->AA: Probable loss of Cdk1-mediated regulation
FT leads to lack of dissociation from chromatin and block of
FT replication resulting in anaphase chromosome bridging
FT during mid-blastula transition in embryos; in the eye
FT imaginal disk, results in complete lethality with pupae
FT developing into headless nearly adult flies; when
FT associated with A-946; 969-A-A-970; A-1002; A-1019; 1031-A-
FT A-1032; A-1035; A-1043; 1047-A-A-1048; A-1067; A-1071; A-
FT 1075; A-1093; A-1102 and A-1140."
FT /evidence="ECO:0000269|PubMed:29746464"
FT MUTAGEN 969..970
FT /note="SS->AA: Probable loss of Cdk1-mediated regulation
FT leads to lack of dissociation from chromatin and block of
FT replication resulting in anaphase chromosome bridging
FT during mid-blastula transition in embryos; in the eye
FT imaginal disk, results in complete lethality with pupae
FT developing into headless nearly adult flies; when
FT associated with A-946; 958-A-A-959; A-1002; A-1019; 1031-A-
FT A-1032; A-1035; A-1043; 1047-A-A-1048; A-1067; A-1071; A-
FT 1075; A-1093; A-1102 and A-1140."
FT /evidence="ECO:0000269|PubMed:29746464"
FT MUTAGEN 1002
FT /note="S->A: Probable loss of Cdk1-mediated regulation
FT leads to lack of dissociation from chromatin and block of
FT replication resulting in anaphase chromosome bridging
FT during mid-blastula transition in embryos; in the eye
FT imaginal disk, results in complete lethality with pupae
FT developing into headless nearly adult flies; when
FT associated with A-946; 958-A-A-959; 969-A-A-970; A-1019;
FT 1031-A-A-1032; A-1035; A-1043; 1047-A-A-1048; A-1067; A-
FT 1071; A-1075; A-1093; A-1102 and A-1140."
FT /evidence="ECO:0000269|PubMed:29746464"
FT MUTAGEN 1019
FT /note="T->A: Probable loss of Cdk1-mediated regulation
FT leads to lack of dissociation from chromatin and block of
FT replication resulting in anaphase chromosome bridging
FT during mid-blastula transition in embryos; in the eye
FT imaginal disk, results in complete lethality with pupae
FT developing into headless nearly adult flies; when
FT associated with A-946; 958-A-A-959; 969-A-A-970; A-1002;
FT 1031-A-A-1032; A-1035; A-1043; 1047-A-A-1048; A-1067; A-
FT 1071; A-1075; A-1093; A-1102 and A-1140."
FT /evidence="ECO:0000269|PubMed:29746464"
FT MUTAGEN 1031..1032
FT /note="ST->AA: Probable loss of Cdk1-mediated regulation
FT leads to lack of dissociation from chromatin and block of
FT replication resulting in anaphase chromosome bridging
FT during mid-blastula transition in embryos; in the eye
FT imaginal disk, results in complete lethality with pupae
FT developing into headless nearly adult flies; when
FT associated with A-946; 958-A-A-959; 969-A-A-970; A-1002; A-
FT 1019; A-1035; A-1043; 1047-A-A-1048; A-1067; A-1071; A-
FT 1075; A-1093; A-1102 and A-1140."
FT /evidence="ECO:0000269|PubMed:29746464"
FT MUTAGEN 1035
FT /note="S->A: Probable loss of Cdk1-mediated regulation
FT leads to lack of dissociation from chromatin and block of
FT replication resulting in anaphase chromosome bridging
FT during mid-blastula transition in embryos; in the eye
FT imaginal disk, results in complete lethality with pupae
FT developing into headless nearly adult flies; when
FT associated with A-946; 958-A-A-959; 969-A-A-970; A-1002; A-
FT 1019; 1031-A-A-1032; A-1043; 1047-A-A-1048; A-1067; A-1071;
FT A-1075; A-1093; A-1102 and A-1140."
FT /evidence="ECO:0000269|PubMed:29746464"
FT MUTAGEN 1043
FT /note="T->A: Probable loss of Cdk1-mediated regulation
FT leads to lack of dissociation from chromatin and block of
FT replication resulting in anaphase chromosome bridging
FT during mid-blastula transition in embryos; in the eye
FT imaginal disk, results in complete lethality with pupae
FT developing into headless nearly adult flies; when
FT associated with A-946; 958-A-A-959; 969-A-A-970; A-1002; A-
FT 1019; 1031-A-A-1032; A-1035; 1047-A-A-1048; A-1067; A-1071;
FT A-1075; A-1093; A-1102 and A-1140."
FT /evidence="ECO:0000269|PubMed:29746464"
FT MUTAGEN 1047..1048
FT /note="ST->AA: Probable loss of Cdk1-mediated regulation
FT leads to lack of dissociation from chromatin and block of
FT replication resulting in anaphase chromosome bridging
FT during mid-blastula transition in embryos; in the eye
FT imaginal disk, results in complete lethality with pupae
FT developing into headless nearly adult flies; when
FT associated with A-946; 958-A-A-959; 969-A-A-970; A-1002; A-
FT 1019; 1031-A-A-1032; A-1035; A-1043; A-1067; A-1071; A-
FT 1075; A-1093; A-1102 and A-1140."
FT /evidence="ECO:0000269|PubMed:29746464"
FT MUTAGEN 1067
FT /note="S->A: Probable loss of Cdk1-mediated regulation
FT leads to lack of dissociation from chromatin and block of
FT replication resulting in anaphase chromosome bridging
FT during mid-blastula transition in embryos; in the eye
FT imaginal disk, results in complete lethality with pupae
FT developing into headless nearly adult flies; when
FT associated with A-946; 958-A-A-959; 969-A-A-970; A-1002; A-
FT 1019; 1031-A-A-1032; A-1035; A-1043; 1047-A-A-1048; A-1071;
FT A-1075; A-1093; A-1102 and A-1140."
FT /evidence="ECO:0000269|PubMed:29746464"
FT MUTAGEN 1071
FT /note="S->A: Probable loss of Cdk1-mediated regulation
FT leads to lack of dissociation from chromatin and block of
FT replication resulting in anaphase chromosome bridging
FT during mid-blastula transition in embryos; in the eye
FT imaginal disk, results in complete lethality with pupae
FT developing into headless nearly adult flies; when
FT associated with A-946; 958-A-A-959; 969-A-A-970; A-1002; A-
FT 1019; 1031-A-A-1032; A-1035; A-1043; 1047-A-A-1048; A-1067;
FT A-1075; A-1093; A-1102 and A-1140."
FT /evidence="ECO:0000269|PubMed:29746464"
FT MUTAGEN 1075
FT /note="S->A: Probable loss of Cdk1-mediated regulation
FT leads to lack of dissociation from chromatin and block of
FT replication resulting in anaphase chromosome bridging
FT during mid-blastula transition in embryos; in the eye
FT imaginal disk, results in complete lethality with pupae
FT developing into headless nearly adult flies; when
FT associated with A-946; 958-A-A-959; 969-A-A-970; A-1002; A-
FT 1019; 1031-A-A-1032; A-1035; A-1043; 1047-A-A-1048; A-1067;
FT A-1071; A-1093; A-1102 and A-1140."
FT /evidence="ECO:0000269|PubMed:29746464"
FT MUTAGEN 1076..1077
FT /note="IL->AA: Significant loss of DNA underreplication in
FT larval salivary glands; when associated with 1101-A--A-
FT 1103."
FT /evidence="ECO:0000269|PubMed:30277458"
FT MUTAGEN 1093
FT /note="S->A: Probable loss of Cdk1-mediated regulation
FT leads to lack of dissociation from chromatin and block of
FT replication resulting in anaphase chromosome bridging
FT during mid-blastula transition in embryos; in the eye
FT imaginal disk, results in complete lethality with pupae
FT developing into headless nearly adult flies; when
FT associated with A-946; 958-A-A-959; 969-A-A-970; A-1002; A-
FT 1019; 1031-A-A-1032; A-1035; A-1043; 1047-A-A-1048; A-1067;
FT A-1071; A-1075; A-1102 and A-1140."
FT /evidence="ECO:0000269|PubMed:29746464"
FT MUTAGEN 1101..1103
FT /note="VSF->ASA: Significant loss of DNA underreplication
FT in larval salivary glands; when associated with 1076-A-A-
FT 1077."
FT /evidence="ECO:0000269|PubMed:30277458"
FT MUTAGEN 1102
FT /note="S->A: Probable loss of Cdk1-mediated regulation
FT leads to lack of dissociation from chromatin and block of
FT replication resulting in anaphase chromosome bridging
FT during mid-blastula transition in embryos; in the eye
FT imaginal disk, results in complete lethality with pupae
FT developing into headless nearly adult flies; when
FT associated with A-946; 958-A-A-959; 969-A-A-970; A-1002; A-
FT 1019; 1031-A-A-1032; A-1035; A-1043; 1047-A-A-1048; A-1067;
FT A-1071; A-1075; A-1093 and A-1140."
FT /evidence="ECO:0000269|PubMed:29746464"
FT MUTAGEN 1140
FT /note="T->A: Probable loss of Cdk1-mediated regulation
FT leads to lack of dissociation from chromatin and block of
FT replication resulting in anaphase chromosome bridging
FT during mid-blastula transition in embryos; in the eye
FT imaginal disk, results in complete lethality with pupae
FT developing into headless nearly adult flies; when
FT associated with A-946; 958-A-A-959; 969-A-A-970; A-1002; A-
FT 1019; 1031-A-A-1032; A-1035; A-1043; 1047-A-A-1048; A-1067;
FT A-1071; A-1075; A-1093 and A-1102."
FT /evidence="ECO:0000269|PubMed:29746464"
FT CONFLICT 474
FT /note="K -> E (in Ref. 4; AAY55130)"
FT /evidence="ECO:0000305"
FT CONFLICT 1049
FT /note="P -> R (in Ref. 4; AAY55130)"
FT /evidence="ECO:0000305"
FT CONFLICT 1156
FT /note="I -> T (in Ref. 4; AAY55130)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 1416 AA; 156820 MW; 307E007CF4B6F83E CRC64;
MDVDFGAQAG QSYRTVQLSA KEVLAAGRRQ HYCNMIDLLA SSCKSSGLIA TSFTDDELVE
FWLGDILPLM FDADRKIQDC AVAALAEALV ALDVSVIHSA KCWPQIRSEF VDKYTIIIGE
MRDAKNSNWH KIWTLLVQIM DEDLLRGCVY INKFLALVEL GFRNPDNGVR SEAFLCWRVL
IKIFAAYDEL TSAKRLRLLL IPLRTSQSRS SHVSGIKLRV WWYLLSCLDH ELPKSFDSAV
EHFLAFMLGG GPRNLATGLA HNYQTARELA LPCLVALWGV ESSEPLQRLM RELRLETLVQ
PSPLMSVEVF QQHWKPLLAA AVSGLKLLTE NDATEAEQLL LQLLVRNLCL AMFRLAVAPF
NVACCSEIEK ILQAEDNSGG RVVRALFNTI AADNLVMERV SGSDHLDVLE AYLKLVLKSK
TEVPAAILQR SIACIFAVDR IEANNQNEFR MLGSFAELLM QANDEEDFEG FAFKLQVWRQ
VSQALSNYLR NNALEYRVAH NASLLDTWLL WPLQTLAAFA GRRASNSFDA SFCDQWRQLV
NAGQNAPGRK KFLTDLKATL TDLLKSKDEP LFAELFDAYV TSLIKFGLCK EAPLYKDVFG
LLQTIFEQPS SQKTLEACLN TLRNLVVELR QNELMVVFDS LKPTLSSGIQ CWNKMKCEGG
FLEEWKRGIQ EKFRKLPMKT MANQLKELFK ADDLFVIIPS VWSLNPEKLT DRQKERFAEK
SDIPALYNDM SQSQDSASIK PWTPKKVVIA KSKQGELALT GKDDNGEVID ITASEESEKE
PVRVDVVTPI RKKPGRKSQA QKELEAAARA ASLAEEPPKR QTRTRAAQKE TEQVAQPQPA
FEPQLRSPKK LPPSPVVQST AAAVKQAKVA KPTPVVVIAQ SEDLFPEVAA EPEPQPEPVK
KPDPVPETTQ LTPPDVPLEA VPSTQLPGSS GDPAASAGAV AAGEMSPKKS GTRIYNLSSP
PDRKQTNNSS SPTLRPKPTG HLTGRGAQLI NMIRNKKLDA ASGSPYACMS TSRLVHQVTP
ARAQDRAEQV STPTSELNEL TGTDHTSTPI QAPPSKDLLV FSKRLPSPSA SPSVSILKRK
LRCESLDDVT LDSPALKRKR VSFHDPPVSV TKEYLRDAEE TRSSLKPKRC LLMDKVAQTT
EMRQALRRRG RLDSIIEIER FASEQTARTA TTDKSLDKST GEAPEEDAFT SLKWNDTGNA
HNISISEEPV KAMEVESELE TAGQDLAIMD PEAALDLAVA QLPLESVLQR YFDKSPLKSA
GMLAKFLSAQ MTANEKLKTN VLETLSENHS KDFLDHAVRE NLSSVVCDRL NPTSVLEYVC
AKSKISASCR NGLLAQVPEI LKSGPRSDSE RLAFVQQLMV QCSPGDDLLL DLIDLLMRTR
RERNNSTSTH ISKGVVSAVG SPDNVAETAA DSSSNL