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RIG_DROME
ID   RIG_DROME               Reviewed;        1235 AA.
AC   Q86BY9; Q86P78; Q9V957;
DT   16-JAN-2004, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2003, sequence version 1.
DT   03-AUG-2022, entry version 141.
DE   RecName: Full=Protein rigor mortis;
GN   Name=rig; ORFNames=CG30149;
OS   Drosophila melanogaster (Fruit fly).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC   Drosophilidae; Drosophila; Sophophora.
OX   NCBI_TaxID=7227;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE
RP   SPECIFICITY, AND INTERACTION WITH ECR; SVP; USP; HR39 AND HR46.
RX   PubMed=14645129; DOI=10.1242/dev.00920;
RA   Gates J., Lam G., Ortiz J.A., Losson R., Thummel C.S.;
RT   "rigor mortis encodes a novel nuclear receptor interacting protein required
RT   for ecdysone signaling during Drosophila larval development.";
RL   Development 131:25-36(2004).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Berkeley;
RX   PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA   Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA   Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA   George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA   Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA   Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA   Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA   An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA   Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA   Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA   Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA   Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA   Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA   Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA   Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA   Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA   Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA   Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA   Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA   Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA   Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA   Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA   McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA   Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA   Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA   Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA   Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA   Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA   Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA   Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA   Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA   Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA   Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA   Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA   Venter J.C.;
RT   "The genome sequence of Drosophila melanogaster.";
RL   Science 287:2185-2195(2000).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=Berkeley;
RX   PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA   Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA   Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA   Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA   Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA   Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA   Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT   "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT   review.";
RL   Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=Berkeley; TISSUE=Embryo;
RX   PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA   Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA   Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA   Celniker S.E.;
RT   "A Drosophila full-length cDNA resource.";
RL   Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN   [5]
RP   PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-289; SER-290; SER-351;
RP   SER-354; THR-355; SER-357 AND THR-411, AND IDENTIFICATION BY MASS
RP   SPECTROMETRY.
RC   TISSUE=Embryo;
RX   PubMed=18327897; DOI=10.1021/pr700696a;
RA   Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P.;
RT   "Phosphoproteome analysis of Drosophila melanogaster embryos.";
RL   J. Proteome Res. 7:1675-1682(2008).
RN   [6]
RP   SUBCELLULAR LOCATION, AND TISSUE SPECIFICITY.
RX   PubMed=20452345; DOI=10.1016/j.yexcr.2010.05.001;
RA   Cauchi R.J., Sanchez-Pulido L., Liu J.L.;
RT   "Drosophila SMN complex proteins Gemin2, Gemin3, and Gemin5 are components
RT   of U bodies.";
RL   Exp. Cell Res. 316:2354-2364(2010).
CC   -!- FUNCTION: Nuclear receptor cofactor for the ecdysone-regulated
CC       processes of molting and puparium formation. Acts downstream from
CC       ecdysone biosynthesis and release to control the expression of specific
CC       ecdysone-regulated genes such as Eip74EF (E74).
CC       {ECO:0000269|PubMed:14645129}.
CC   -!- SUBUNIT: Interacts with nuclear receptors EcR, svp (seven up), usp
CC       (ultraspiracle), Hr39 and Hr3. {ECO:0000269|PubMed:14645129}.
CC   -!- INTERACTION:
CC       Q86BY9; Q05192: Hr39; NbExp=2; IntAct=EBI-422757, EBI-75048;
CC   -!- SUBCELLULAR LOCATION: Nucleus. Cytoplasm. Note=During second and third
CC       instar larval development, it is localized primarily to the cytoplasm
CC       of cells in the brain and imaginal disks (tissues that are fated to
CC       form specific parts of the adult fly during metamorphosis). Also
CC       localized to the cytoplasm of larval salivary gland cells during the
CC       second and early third instar stages. It however, begins to shift into
CC       the nucleus of these cells in the mid-third instar maintaining this
CC       localization through the end of larval development. At puparium
CC       formation, it shuttles out of the nucleus of salivary gland cells to
CC       become more abundant in the cytoplasm. A similar dynamic movement
CC       occurs in cells of the larval midgut during third instar development.
CC       Component of U bodies.
CC   -!- TISSUE SPECIFICITY: Expressed in the brain and salivary glands of early
CC       and late second instar larvae. Expressed in nurse cells and oocytes.
CC       {ECO:0000269|PubMed:14645129, ECO:0000269|PubMed:20452345}.
CC   -!- DOMAIN: Contains one Leu-Xaa-Xaa-Leu-Leu (LXXLL) motif, which is
CC       usually essential for the association with nuclear receptors.
CC       {ECO:0000250}.
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DR   EMBL; AY274835; AAP31582.1; -; mRNA.
DR   EMBL; AE013599; AAF57440.3; -; Genomic_DNA.
DR   EMBL; BT003437; AAO39440.1; -; mRNA.
DR   RefSeq; NP_611499.3; NM_137655.3.
DR   AlphaFoldDB; Q86BY9; -.
DR   BioGRID; 62984; 7.
DR   IntAct; Q86BY9; 6.
DR   STRING; 7227.FBpp0085564; -.
DR   iPTMnet; Q86BY9; -.
DR   PaxDb; Q86BY9; -.
DR   PRIDE; Q86BY9; -.
DR   EnsemblMetazoa; FBtr0086252; FBpp0085564; FBgn0250850.
DR   GeneID; 37335; -.
DR   KEGG; dme:Dmel_CG30149; -.
DR   CTD; 37335; -.
DR   FlyBase; FBgn0250850; rig.
DR   VEuPathDB; VectorBase:FBgn0250850; -.
DR   eggNOG; KOG4155; Eukaryota.
DR   HOGENOM; CLU_279538_0_0_1; -.
DR   InParanoid; Q86BY9; -.
DR   OMA; DVSPMWG; -.
DR   OrthoDB; 283127at2759; -.
DR   PhylomeDB; Q86BY9; -.
DR   SignaLink; Q86BY9; -.
DR   BioGRID-ORCS; 37335; 0 hits in 1 CRISPR screen.
DR   GenomeRNAi; 37335; -.
DR   PRO; PR:Q86BY9; -.
DR   Proteomes; UP000000803; Chromosome 2R.
DR   Bgee; FBgn0250850; Expressed in ovary and 13 other tissues.
DR   Genevisible; Q86BY9; DM.
DR   GO; GO:0005737; C:cytoplasm; IDA:UniProtKB.
DR   GO; GO:0071254; C:cytoplasmic U snRNP body; IDA:FlyBase.
DR   GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR   GO; GO:0032797; C:SMN complex; IDA:FlyBase.
DR   GO; GO:0003730; F:mRNA 3'-UTR binding; IBA:GO_Central.
DR   GO; GO:0008344; P:adult locomotory behavior; IMP:FlyBase.
DR   GO; GO:0007629; P:flight behavior; IMP:FlyBase.
DR   GO; GO:0007591; P:molting cycle, chitin-based cuticle; IMP:UniProtKB.
DR   GO; GO:0035075; P:response to ecdysone; IMP:UniProtKB.
DR   GO; GO:0000387; P:spliceosomal snRNP assembly; ISS:FlyBase.
DR   Gene3D; 2.130.10.10; -; 3.
DR   InterPro; IPR024977; Apc4_WD40_dom.
DR   InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR   InterPro; IPR001680; WD40_repeat.
DR   InterPro; IPR036322; WD40_repeat_dom_sf.
DR   Pfam; PF12894; ANAPC4_WD40; 1.
DR   SMART; SM00320; WD40; 7.
DR   SUPFAM; SSF50978; SSF50978; 3.
DR   PROSITE; PS00678; WD_REPEATS_1; 1.
PE   1: Evidence at protein level;
KW   Cytoplasm; Developmental protein; Nucleus; Phosphoprotein;
KW   Reference proteome; Repeat; Transcription; Transcription regulation;
KW   WD repeat.
FT   CHAIN           1..1235
FT                   /note="Protein rigor mortis"
FT                   /id="PRO_0000051199"
FT   REPEAT          55..102
FT                   /note="WD 1"
FT   REPEAT          430..469
FT                   /note="WD 2"
FT   REPEAT          475..512
FT                   /note="WD 3"
FT   REPEAT          565..605
FT                   /note="WD 4"
FT   REPEAT          611..650
FT                   /note="WD 5"
FT   REPEAT          690..730
FT                   /note="WD 6"
FT   REPEAT          739..779
FT                   /note="WD 7"
FT   REPEAT          788..828
FT                   /note="WD 8"
FT   REGION          227..263
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          340..368
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          963..983
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOTIF           443..447
FT                   /note="LXXLL motif"
FT   COMPBIAS        238..252
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        348..368
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        963..982
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOD_RES         289
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000269|PubMed:18327897"
FT   MOD_RES         290
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000269|PubMed:18327897"
FT   MOD_RES         351
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000269|PubMed:18327897"
FT   MOD_RES         354
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000269|PubMed:18327897"
FT   MOD_RES         355
FT                   /note="Phosphothreonine"
FT                   /evidence="ECO:0000269|PubMed:18327897"
FT   MOD_RES         357
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000269|PubMed:18327897"
FT   MOD_RES         411
FT                   /note="Phosphothreonine"
FT                   /evidence="ECO:0000269|PubMed:18327897"
FT   CONFLICT        119
FT                   /note="H -> N (in Ref. 4; AAO39440)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        126
FT                   /note="L -> V (in Ref. 4; AAO39440)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        321
FT                   /note="E -> D (in Ref. 4; AAO39440)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        334
FT                   /note="V -> L (in Ref. 4; AAO39440)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        364
FT                   /note="D -> E (in Ref. 4; AAO39440)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        417
FT                   /note="E -> K (in Ref. 4; AAO39440)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        516
FT                   /note="S -> T (in Ref. 4; AAO39440)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        731
FT                   /note="S -> T (in Ref. 4; AAO39440)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        764
FT                   /note="R -> K (in Ref. 4; AAO39440)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        941
FT                   /note="P -> S (in Ref. 4; AAO39440)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        947
FT                   /note="T -> S (in Ref. 4; AAO39440)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        1029
FT                   /note="S -> T (in Ref. 4; AAO39440)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   1235 AA;  137829 MW;  015E185A6941F451 CRC64;
     MNSQVMYHNV PTPPGNVSLA AAAPDGGLLY AGIRCINYIS APPANGEQPQ VVTMSTRINI
     LALDVSPMWG LGNGGPTKPF AIVGDDLSVQ VWDCALGEAV IGHKAHQHQH EARDVRVVHH
     TTNSVLMSYL ANGNILSMDA SDLVIYCVAS NTYCRRSTFI SPRNHQLTMV RCSPYNDNLF
     AVGTAMGNVL VCDLRKMNIV YKFHGHKAPI CGLAWREVPA AEDEKTNNLA LSAEEWRSRN
     GGQEEKPKTK PPPLTKSKAA ESDDPFDIYN FDHLEYEFGA PIAERRRKSS EDCGGEFVGL
     EKPAGAAVLD FVEACESVKA ELLASRQEDK TQHVEVTLHD CEPTKPTGPL SDASTISNKN
     DASDSTEGSL EVIQYSSSSD DAVIVDGEAA KPKREVLHHI YHQAEVHASG TPQTKSEPQS
     NLQVVPAISA ETISLTSVNS THLETLLVSI DGDEVMMIWN TNTGAHAGKN YSKSKTAGKL
     NNVYWLNNHV IVSLSRHQLF FWSVEFERKM LRYKISKDKS HSCHLQDIVS FACDSSKEMI
     WLCRNNRQIG MMNPKTGRMA DFYGTVAFGV RAMAECPDDM NKIALGCSDR RVAFFDISKL
     TTSCLPIDSV YVSSNVYCLA WSPNCLELAF GTFDGTVGIL DVERMKVKTH LRTPHKKEVY
     SLVWQDHFIY FIVNRVLGFF DLRKSKIEPT IVNCISRPSY LSIRDSFLFV GTDDGLLQIH
     ERDSGMEKSW SPFIRQSALF ARYVTDIAWC PLDSNKFAVS GNDRSVYVME FQPTERNWKT
     LHTFTANTEK ASITSMRWSH TQKHLLLTFH IEGKVCLWNC NAPEKPPLTI TYHCPMWCGM
     FLPTNENIIM CSGKALSVEL IDIKDALEGD EKSICPKVDA LLNVKWASKS LTQPYAPVLT
     AAEKKRQRRD QRKAAAKLEV DVANKDQKKI QESVTAVIDN PTNDKCTQET PVEEMLEALS
     LDKEQNNRSA KECPKCKEQS PDSFTHSRTC LYLTQKELNK SALEKLAIVL TEDSAKIDKS
     VLISKLFSSK VMAKELIATE LTNLKHSNTK DIAPLCLAMS TFKLREELEQ HIANKTLTER
     HVSLAPSVSF TLWQDCCRAY AKQMEEKGYI MHAATYLFSQ GMQSEAIKLF LANEYYKEAL
     VHARICLPAT DPLIKTVINN WLEHLEGTGN FAAAALICVL DNEMLRGYSY LRKYRNCTPE
     IADLMDQIKR IGQLGGVLDG CAPNEPIHNG STAEH
 
 
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