ID   RIHA_ECO5E              Reviewed;         311 AA.
AC   B5YQK2;
DT   14-APR-2009, integrated into UniProtKB/Swiss-Prot.
DT   25-NOV-2008, sequence version 1.
DT   25-MAY-2022, entry version 65.
DE   RecName: Full=Pyrimidine-specific ribonucleoside hydrolase RihA {ECO:0000255|HAMAP-Rule:MF_01431};
DE            EC=3.2.-.- {ECO:0000255|HAMAP-Rule:MF_01431};
DE   AltName: Full=Cytidine/uridine-specific hydrolase {ECO:0000255|HAMAP-Rule:MF_01431};
GN   Name=rihA {ECO:0000255|HAMAP-Rule:MF_01431};
GN   OrderedLocusNames=ECH74115_0743;
OS   Escherichia coli O157:H7 (strain EC4115 / EHEC).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=444450;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=EC4115 / EHEC;
RX   PubMed=22135463; DOI=10.1073/pnas.1107176108;
RA   Eppinger M., Mammel M.K., Leclerc J.E., Ravel J., Cebula T.A.;
RT   "Genomic anatomy of Escherichia coli O157:H7 outbreaks.";
RL   Proc. Natl. Acad. Sci. U.S.A. 108:20142-20147(2011).
CC   -!- FUNCTION: Hydrolyzes with equal efficiency cytidine or uridine to
CC       ribose and cytosine or uracil, respectively. {ECO:0000255|HAMAP-
CC       Rule:MF_01431}.
CC   -!- SIMILARITY: Belongs to the IUNH family. RihA subfamily.
CC       {ECO:0000255|HAMAP-Rule:MF_01431}.
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DR   EMBL; CP001164; ACI35254.1; -; Genomic_DNA.
DR   RefSeq; WP_001207527.1; NC_011353.1.
DR   AlphaFoldDB; B5YQK2; -.
DR   SMR; B5YQK2; -.
DR   GeneID; 58461701; -.
DR   KEGG; ecf:ECH74115_0743; -.
DR   HOGENOM; CLU_036838_2_0_6; -.
DR   OMA; HAPDIHG; -.
DR   GO; GO:0045437; F:uridine nucleosidase activity; IEA:InterPro.
DR   GO; GO:0015949; P:nucleobase-containing small molecule interconversion; IEA:InterPro.
DR   GO; GO:0006206; P:pyrimidine nucleobase metabolic process; IEA:UniProtKB-UniRule.
DR   Gene3D; 3.90.245.10; -; 1.
DR   HAMAP; MF_01431; Pyrim_hydro_RihA; 1.
DR   InterPro; IPR015910; I/U_nuclsd_hydro_CS.
DR   InterPro; IPR001910; Inosine/uridine_hydrolase_dom.
DR   InterPro; IPR023186; IUNH.
DR   InterPro; IPR022975; Pyrim_hydro_RihA.
DR   InterPro; IPR036452; Ribo_hydro-like.
DR   PANTHER; PTHR12304; PTHR12304; 1.
DR   Pfam; PF01156; IU_nuc_hydro; 1.
DR   SUPFAM; SSF53590; SSF53590; 1.
DR   PROSITE; PS01247; IUNH; 1.
PE   3: Inferred from homology;
KW   Glycosidase; Hydrolase.
FT   CHAIN           1..311
FT                   /note="Pyrimidine-specific ribonucleoside hydrolase RihA"
FT                   /id="PRO_1000145790"
FT   ACT_SITE        240
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01431"
SQ   SEQUENCE   311 AA;  33837 MW;  99B6C88B60BABDD1 CRC64;
     MALPILLDCD PGHDDAIAIV LALASPELDV KAITSSAGNQ TPEKTLRNVL RMLTLLNRTD
     IPVAGGAVKP LMRELIIADN VHGESGLDGP ALPEPTFAPQ NCTAVELMAK TLRESAEPVT
     IVSTGPQTNV ALLLNSHPEL HSKIARIVIM GGAMGLGNWT PAAEFNIYVD PEAAEIVFQS
     GIPVVMAGLD VTHKAQIHVE DTERFRAIGN PVSTIVAELL DFFLEYHKDE KWGFVGAPLH
     DPCTIAWLLK PELFTTVERW VGVETQGKYT QGMTVVDYYY LTGNKPNATV MVDVDRQGFV
     DLLADRLKFY A