RIHA_ECOLI
ID RIHA_ECOLI Reviewed; 311 AA.
AC P41409; P77738;
DT 01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1997, sequence version 2.
DT 03-AUG-2022, entry version 153.
DE RecName: Full=Pyrimidine-specific ribonucleoside hydrolase RihA;
DE EC=3.2.-.-;
DE AltName: Full=Cytidine/uridine-specific hydrolase;
GN Name=rihA; Synonyms=ybeK; OrderedLocusNames=b0651, JW0646;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=8905232; DOI=10.1093/dnares/3.3.137;
RA Oshima T., Aiba H., Baba T., Fujita K., Hayashi K., Honjo A., Ikemoto K.,
RA Inada T., Itoh T., Kajihara M., Kanai K., Kashimoto K., Kimura S.,
RA Kitagawa M., Makino K., Masuda S., Miki T., Mizobuchi K., Mori H.,
RA Motomura K., Nakamura Y., Nashimoto H., Nishio Y., Saito N., Sampei G.,
RA Seki Y., Tagami H., Takemoto K., Wada C., Yamamoto Y., Yano M.,
RA Horiuchi T.;
RT "A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to
RT the 12.7-28.0 min region on the linkage map.";
RL DNA Res. 3:137-155(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RA Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M.,
RA Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D.,
RA Namath A., Oefner P., Roberts D., Schramm S., Davis R.W.;
RT "Sequence of minutes 4-25 of Escherichia coli.";
RL Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [5]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-123.
RC STRAIN=K12 / BK9MDG;
RA Lum D., Wallace B.J.;
RL Submitted (JUN-1994) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP CHARACTERIZATION.
RX PubMed=11027694; DOI=10.1074/jbc.m008300200;
RA Petersen C., Moeller L.B.;
RT "The RihA, RihB, and RihC ribonucleoside hydrolases of Escherichia coli.
RT Substrate specificity, gene expression, and regulation.";
RL J. Biol. Chem. 276:884-894(2001).
CC -!- FUNCTION: Hydrolyzes with equal efficiency cytidine or uridine to
CC ribose and cytosine or uracil, respectively.
CC -!- ACTIVITY REGULATION: Subject to catabolite repression.
CC -!- MISCELLANEOUS: Strictly specific for ribonucleosides.
CC -!- SIMILARITY: Belongs to the IUNH family. RihA subfamily. {ECO:0000305}.
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DR EMBL; U82598; AAB40852.1; -; Genomic_DNA.
DR EMBL; U00096; AAC73752.1; -; Genomic_DNA.
DR EMBL; AP009048; BAA35303.1; -; Genomic_DNA.
DR EMBL; U10981; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR PIR; A64800; A64800.
DR RefSeq; NP_415184.1; NC_000913.3.
DR RefSeq; WP_001207520.1; NZ_SSZK01000037.1.
DR PDB; 1YOE; X-ray; 1.78 A; A=2-311.
DR PDB; 3G5I; X-ray; 2.10 A; A/B/C/D=2-311.
DR PDBsum; 1YOE; -.
DR PDBsum; 3G5I; -.
DR AlphaFoldDB; P41409; -.
DR SMR; P41409; -.
DR BioGRID; 4261645; 16.
DR IntAct; P41409; 15.
DR STRING; 511145.b0651; -.
DR jPOST; P41409; -.
DR PaxDb; P41409; -.
DR PRIDE; P41409; -.
DR EnsemblBacteria; AAC73752; AAC73752; b0651.
DR EnsemblBacteria; BAA35303; BAA35303; BAA35303.
DR GeneID; 945503; -.
DR KEGG; ecj:JW0646; -.
DR KEGG; eco:b0651; -.
DR PATRIC; fig|1411691.4.peg.1617; -.
DR EchoBASE; EB2563; -.
DR eggNOG; COG1957; Bacteria.
DR HOGENOM; CLU_036838_2_0_6; -.
DR InParanoid; P41409; -.
DR OMA; HAPDIHG; -.
DR PhylomeDB; P41409; -.
DR BioCyc; EcoCyc:G6358-MON; -.
DR BioCyc; MetaCyc:G6358-MON; -.
DR EvolutionaryTrace; P41409; -.
DR PRO; PR:P41409; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR GO; GO:0032991; C:protein-containing complex; IDA:EcoCyc.
DR GO; GO:0005509; F:calcium ion binding; IDA:EcoliWiki.
DR GO; GO:0008477; F:purine nucleosidase activity; IBA:GO_Central.
DR GO; GO:0050263; F:ribosylpyrimidine nucleosidase activity; IDA:EcoCyc.
DR GO; GO:0045437; F:uridine nucleosidase activity; IDA:EcoCyc.
DR GO; GO:0015949; P:nucleobase-containing small molecule interconversion; IEA:InterPro.
DR GO; GO:0006152; P:purine nucleoside catabolic process; IBA:GO_Central.
DR GO; GO:0006206; P:pyrimidine nucleobase metabolic process; IEA:UniProtKB-UniRule.
DR GO; GO:0046133; P:pyrimidine ribonucleoside catabolic process; IMP:EcoCyc.
DR Gene3D; 3.90.245.10; -; 1.
DR HAMAP; MF_01431; Pyrim_hydro_RihA; 1.
DR InterPro; IPR015910; I/U_nuclsd_hydro_CS.
DR InterPro; IPR001910; Inosine/uridine_hydrolase_dom.
DR InterPro; IPR023186; IUNH.
DR InterPro; IPR022975; Pyrim_hydro_RihA.
DR InterPro; IPR036452; Ribo_hydro-like.
DR PANTHER; PTHR12304; PTHR12304; 1.
DR Pfam; PF01156; IU_nuc_hydro; 1.
DR SUPFAM; SSF53590; SSF53590; 1.
DR PROSITE; PS01247; IUNH; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Glycosidase; Hydrolase; Reference proteome.
FT CHAIN 1..311
FT /note="Pyrimidine-specific ribonucleoside hydrolase RihA"
FT /id="PRO_0000206813"
FT ACT_SITE 240
FT /evidence="ECO:0000250"
FT STRAND 3..9
FT /evidence="ECO:0007829|PDB:1YOE"
FT HELIX 13..23
FT /evidence="ECO:0007829|PDB:1YOE"
FT STRAND 28..35
FT /evidence="ECO:0007829|PDB:1YOE"
FT STRAND 37..40
FT /evidence="ECO:0007829|PDB:1YOE"
FT HELIX 42..55
FT /evidence="ECO:0007829|PDB:1YOE"
FT STRAND 63..65
FT /evidence="ECO:0007829|PDB:1YOE"
FT STRAND 71..73
FT /evidence="ECO:0007829|PDB:1YOE"
FT TURN 79..81
FT /evidence="ECO:0007829|PDB:3G5I"
FT TURN 83..85
FT /evidence="ECO:0007829|PDB:3G5I"
FT HELIX 104..114
FT /evidence="ECO:0007829|PDB:1YOE"
FT STRAND 119..123
FT /evidence="ECO:0007829|PDB:1YOE"
FT HELIX 128..136
FT /evidence="ECO:0007829|PDB:1YOE"
FT HELIX 138..143
FT /evidence="ECO:0007829|PDB:1YOE"
FT STRAND 144..150
FT /evidence="ECO:0007829|PDB:1YOE"
FT STRAND 158..162
FT /evidence="ECO:0007829|PDB:1YOE"
FT HELIX 165..169
FT /evidence="ECO:0007829|PDB:1YOE"
FT HELIX 171..178
FT /evidence="ECO:0007829|PDB:1YOE"
FT STRAND 180..182
FT /evidence="ECO:0007829|PDB:1YOE"
FT STRAND 184..187
FT /evidence="ECO:0007829|PDB:1YOE"
FT HELIX 189..192
FT /evidence="ECO:0007829|PDB:1YOE"
FT STRAND 195..197
FT /evidence="ECO:0007829|PDB:1YOE"
FT HELIX 199..208
FT /evidence="ECO:0007829|PDB:1YOE"
FT HELIX 211..224
FT /evidence="ECO:0007829|PDB:1YOE"
FT STRAND 235..238
FT /evidence="ECO:0007829|PDB:1YOE"
FT HELIX 242..249
FT /evidence="ECO:0007829|PDB:1YOE"
FT HELIX 251..253
FT /evidence="ECO:0007829|PDB:1YOE"
FT STRAND 254..259
FT /evidence="ECO:0007829|PDB:1YOE"
FT STRAND 261..263
FT /evidence="ECO:0007829|PDB:1YOE"
FT STRAND 274..276
FT /evidence="ECO:0007829|PDB:1YOE"
FT STRAND 288..294
FT /evidence="ECO:0007829|PDB:1YOE"
FT HELIX 296..307
FT /evidence="ECO:0007829|PDB:1YOE"
FT HELIX 308..310
FT /evidence="ECO:0007829|PDB:1YOE"
SQ SEQUENCE 311 AA; 33823 MW; EEB6CF8C60BABDD4 CRC64;
MALPILLDCD PGHDDAIAIV LALASPELDV KAITSSAGNQ TPEKTLRNVL RMLTLLNRTD
IPVAGGAVKP LMRELIIADN VHGESGLDGP ALPEPTFAPQ NCTAVELMAK TLRESAEPVT
IVSTGPQTNV ALLLNSHPEL HSKIARIVIM GGAMGLGNWT PAAEFNIYVD PEAAEIVFQS
GIPVVMAGLD VTHKAQIHVE DTERFRAIGN PVSTIVAELL DFFLEYHKDE KWGFVGAPLH
DPCTIAWLLK PELFTSVERW VGVETQGKYT QGMTVVDYYY LTGNKPNATV MVDVDRQGFV
DLLADRLKFY A
