ID   RIHC_ECO5E              Reviewed;         304 AA.
AC   B5YYC2;
DT   14-APR-2009, integrated into UniProtKB/Swiss-Prot.
DT   25-NOV-2008, sequence version 1.
DT   03-AUG-2022, entry version 60.
DE   RecName: Full=Non-specific ribonucleoside hydrolase RihC {ECO:0000255|HAMAP-Rule:MF_01432};
DE            EC=3.2.-.- {ECO:0000255|HAMAP-Rule:MF_01432};
DE   AltName: Full=Purine/pyrimidine ribonucleoside hydrolase {ECO:0000255|HAMAP-Rule:MF_01432};
GN   Name=rihC {ECO:0000255|HAMAP-Rule:MF_01432};
GN   OrderedLocusNames=ECH74115_0032;
OS   Escherichia coli O157:H7 (strain EC4115 / EHEC).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=444450;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=EC4115 / EHEC;
RX   PubMed=22135463; DOI=10.1073/pnas.1107176108;
RA   Eppinger M., Mammel M.K., Leclerc J.E., Ravel J., Cebula T.A.;
RT   "Genomic anatomy of Escherichia coli O157:H7 outbreaks.";
RL   Proc. Natl. Acad. Sci. U.S.A. 108:20142-20147(2011).
CC   -!- FUNCTION: Hydrolyzes both purine and pyrimidine ribonucleosides with a
CC       broad-substrate specificity. {ECO:0000255|HAMAP-Rule:MF_01432}.
CC   -!- SIMILARITY: Belongs to the IUNH family. RihC subfamily.
CC       {ECO:0000255|HAMAP-Rule:MF_01432}.
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DR   EMBL; CP001164; ACI39179.1; -; Genomic_DNA.
DR   RefSeq; WP_001239143.1; NC_011353.1.
DR   AlphaFoldDB; B5YYC2; -.
DR   SMR; B5YYC2; -.
DR   KEGG; ecf:ECH74115_0032; -.
DR   HOGENOM; CLU_036838_2_2_6; -.
DR   OMA; TPVAEWN; -.
DR   GO; GO:0045437; F:uridine nucleosidase activity; IEA:UniProt.
DR   GO; GO:0006144; P:purine nucleobase metabolic process; IEA:UniProtKB-UniRule.
DR   GO; GO:0006206; P:pyrimidine nucleobase metabolic process; IEA:UniProtKB-UniRule.
DR   Gene3D; 3.90.245.10; -; 1.
DR   HAMAP; MF_01432; Nucleosid_hydro_RihC; 1.
DR   InterPro; IPR015910; I/U_nuclsd_hydro_CS.
DR   InterPro; IPR001910; Inosine/uridine_hydrolase_dom.
DR   InterPro; IPR023186; IUNH.
DR   InterPro; IPR022976; Nucleosid_hydro_RihC_nonspecif.
DR   InterPro; IPR036452; Ribo_hydro-like.
DR   PANTHER; PTHR12304; PTHR12304; 1.
DR   Pfam; PF01156; IU_nuc_hydro; 1.
DR   SUPFAM; SSF53590; SSF53590; 1.
DR   PROSITE; PS01247; IUNH; 1.
PE   3: Inferred from homology;
KW   Glycosidase; Hydrolase.
FT   CHAIN           1..304
FT                   /note="Non-specific ribonucleoside hydrolase RihC"
FT                   /id="PRO_1000145809"
FT   ACT_SITE        233
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01432"
SQ   SEQUENCE   304 AA;  32598 MW;  013AA78DF5C6F70F CRC64;
     MRLPIFLDTD PGIDDAVAIA AAIFAPELDL QLMTTVAGNV SVEKTTRNAL QLLHFWNAEI
     PLAQGAAVPL VRAPRDAASV HGESGMAGYD FVEHNRKPLG IPAFLAIRDA LMRAPEPVTL
     VAIGPLTNIA LLLSQCPECK PYIRRLVIMG GSAGRGNCTP NAEFNIAADP EAAACVFRSG
     IEIVMCGLDV TNQAILTPDY LSTLPQLNRT GKMLHALFSH YRSGSMQSGL RMHDLCAIAW
     LVRPDLFTLK PCFVAVETQG EFTSGTTVVD IDGCLGKPAN VQVALDLNVK GFQQWVAEVL
     ALVP