RIHC_ECOLI
ID RIHC_ECOLI Reviewed; 304 AA.
AC P22564;
DT 01-AUG-1991, integrated into UniProtKB/Swiss-Prot.
DT 01-AUG-1991, sequence version 1.
DT 03-AUG-2022, entry version 156.
DE RecName: Full=Non-specific ribonucleoside hydrolase RihC;
DE EC=3.2.-.-;
DE AltName: Full=Purine/pyrimidine ribonucleoside hydrolase;
GN Name=rihC; Synonyms=yaaF; OrderedLocusNames=b0030, JW0028;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=K12;
RX PubMed=2011499; DOI=10.1093/nar/19.1.180;
RA Bouvier J., Stragier P.;
RT "Nucleotide sequence of the lsp-dapB interval in Escherichia coli.";
RL Nucleic Acids Res. 19:180-180(1991).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12;
RX PubMed=1630901; DOI=10.1093/nar/20.13.3305;
RA Yura T., Mori H., Nagai H., Nagata T., Ishihama A., Fujita N., Isono K.,
RA Mizobuchi K., Nakata A.;
RT "Systematic sequencing of the Escherichia coli genome: analysis of the 0-
RT 2.4 min region.";
RL Nucleic Acids Res. 20:3305-3308(1992).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [5]
RP CHARACTERIZATION.
RX PubMed=11027694; DOI=10.1074/jbc.m008300200;
RA Petersen C., Moeller L.B.;
RT "The RihA, RihB, and RihC ribonucleoside hydrolases of Escherichia coli.
RT Substrate specificity, gene expression, and regulation.";
RL J. Biol. Chem. 276:884-894(2001).
CC -!- FUNCTION: Hydrolyzes both purine and pyrimidine ribonucleosides with a
CC broad-substrate specificity with decreasing activity in the order
CC uridine, xanthosine, inosine, adenosine, cytidine, guanosine.
CC -!- ACTIVITY REGULATION: Subject to catabolite repression.
CC -!- MISCELLANEOUS: Strictly specific for ribonucleosides.
CC -!- SIMILARITY: Belongs to the IUNH family. RihC subfamily. {ECO:0000305}.
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DR EMBL; X54945; CAA38708.1; -; Genomic_DNA.
DR EMBL; U00096; AAC73141.1; -; Genomic_DNA.
DR EMBL; AP009048; BAB96599.1; -; Genomic_DNA.
DR PIR; JE0404; JE0404.
DR RefSeq; NP_414571.1; NC_000913.3.
DR RefSeq; WP_001239142.1; NZ_LN832404.1.
DR AlphaFoldDB; P22564; -.
DR SMR; P22564; -.
DR BioGRID; 4261497; 10.
DR BioGRID; 849197; 1.
DR DIP; DIP-11157N; -.
DR IntAct; P22564; 2.
DR STRING; 511145.b0030; -.
DR jPOST; P22564; -.
DR PaxDb; P22564; -.
DR PRIDE; P22564; -.
DR EnsemblBacteria; AAC73141; AAC73141; b0030.
DR EnsemblBacteria; BAB96599; BAB96599; BAB96599.
DR GeneID; 944796; -.
DR KEGG; ecj:JW0028; -.
DR KEGG; eco:b0030; -.
DR PATRIC; fig|1411691.4.peg.2255; -.
DR EchoBASE; EB1074; -.
DR eggNOG; COG1957; Bacteria.
DR HOGENOM; CLU_036838_2_2_6; -.
DR InParanoid; P22564; -.
DR OMA; TPVAEWN; -.
DR PhylomeDB; P22564; -.
DR BioCyc; EcoCyc:EG11082-MON; -.
DR BioCyc; MetaCyc:EG11082-MON; -.
DR PRO; PR:P22564; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR GO; GO:0047622; F:adenosine nucleosidase activity; IDA:EcoCyc.
DR GO; GO:0047724; F:inosine nucleosidase activity; IDA:EcoCyc.
DR GO; GO:0008477; F:purine nucleosidase activity; IBA:GO_Central.
DR GO; GO:0050263; F:ribosylpyrimidine nucleosidase activity; IDA:EcoCyc.
DR GO; GO:0045437; F:uridine nucleosidase activity; IDA:EcoCyc.
DR GO; GO:0006974; P:cellular response to DNA damage stimulus; IEP:EcoliWiki.
DR GO; GO:0006144; P:purine nucleobase metabolic process; IEA:UniProtKB-UniRule.
DR GO; GO:0006152; P:purine nucleoside catabolic process; IBA:GO_Central.
DR GO; GO:0006206; P:pyrimidine nucleobase metabolic process; IEA:UniProtKB-UniRule.
DR GO; GO:0042454; P:ribonucleoside catabolic process; IDA:EcoCyc.
DR Gene3D; 3.90.245.10; -; 1.
DR HAMAP; MF_01432; Nucleosid_hydro_RihC; 1.
DR InterPro; IPR015910; I/U_nuclsd_hydro_CS.
DR InterPro; IPR001910; Inosine/uridine_hydrolase_dom.
DR InterPro; IPR023186; IUNH.
DR InterPro; IPR022976; Nucleosid_hydro_RihC_nonspecif.
DR InterPro; IPR036452; Ribo_hydro-like.
DR PANTHER; PTHR12304; PTHR12304; 1.
DR Pfam; PF01156; IU_nuc_hydro; 1.
DR SUPFAM; SSF53590; SSF53590; 1.
DR PROSITE; PS01247; IUNH; 1.
PE 1: Evidence at protein level;
KW Glycosidase; Hydrolase; Reference proteome.
FT CHAIN 1..304
FT /note="Non-specific ribonucleoside hydrolase RihC"
FT /id="PRO_0000206831"
FT ACT_SITE 233
FT /evidence="ECO:0000250"
SQ SEQUENCE 304 AA; 32561 MW; 0E98D78DF5C6F70F CRC64;
MRLPIFLDTD PGIDDAVAIA AAIFAPELDL QLMTTVAGNV SVEKTTRNAL QLLHFWNAEI
PLAQGAAVPL VRAPRDAASV HGESGMAGYD FVEHNRKPLG IPAFLAIRDA LMRAPEPVTL
VAIGPLTNIA LLLSQCPECK PYIRRLVIMG GSAGRGNCTP NAEFNIAADP EAAACVFRSG
IEIVMCGLDV TNQAILTPDY LSTLPQLNRT GKMLHALFSH YRSGSMQSGL RMHDLCAIAW
LVRPDLFTLK PCFVAVETQG EFTSGTTVVD IDGCLGKPAN VQVALDLDVK GFQQWVAEVL
ALAS
