ID   RIHC_SHIFL              Reviewed;         304 AA.
AC   Q83MH0; Q7C3C2;
DT   10-JAN-2006, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2003, sequence version 1.
DT   03-AUG-2022, entry version 107.
DE   RecName: Full=Non-specific ribonucleoside hydrolase RihC {ECO:0000255|HAMAP-Rule:MF_01432};
DE            EC=3.2.-.- {ECO:0000255|HAMAP-Rule:MF_01432};
DE   AltName: Full=Purine/pyrimidine ribonucleoside hydrolase {ECO:0000255|HAMAP-Rule:MF_01432};
GN   Name=rihC {ECO:0000255|HAMAP-Rule:MF_01432};
GN   OrderedLocusNames=SF0027, S0029;
OS   Shigella flexneri.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Shigella.
OX   NCBI_TaxID=623;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=301 / Serotype 2a;
RX   PubMed=12384590; DOI=10.1093/nar/gkf566;
RA   Jin Q., Yuan Z., Xu J., Wang Y., Shen Y., Lu W., Wang J., Liu H., Yang J.,
RA   Yang F., Zhang X., Zhang J., Yang G., Wu H., Qu D., Dong J., Sun L.,
RA   Xue Y., Zhao A., Gao Y., Zhu J., Kan B., Ding K., Chen S., Cheng H.,
RA   Yao Z., He B., Chen R., Ma D., Qiang B., Wen Y., Hou Y., Yu J.;
RT   "Genome sequence of Shigella flexneri 2a: insights into pathogenicity
RT   through comparison with genomes of Escherichia coli K12 and O157.";
RL   Nucleic Acids Res. 30:4432-4441(2002).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700930 / 2457T / Serotype 2a;
RX   PubMed=12704152; DOI=10.1128/iai.71.5.2775-2786.2003;
RA   Wei J., Goldberg M.B., Burland V., Venkatesan M.M., Deng W., Fournier G.,
RA   Mayhew G.F., Plunkett G. III, Rose D.J., Darling A., Mau B., Perna N.T.,
RA   Payne S.M., Runyen-Janecky L.J., Zhou S., Schwartz D.C., Blattner F.R.;
RT   "Complete genome sequence and comparative genomics of Shigella flexneri
RT   serotype 2a strain 2457T.";
RL   Infect. Immun. 71:2775-2786(2003).
CC   -!- FUNCTION: Hydrolyzes both purine and pyrimidine ribonucleosides with a
CC       broad-substrate specificity. {ECO:0000255|HAMAP-Rule:MF_01432}.
CC   -!- SIMILARITY: Belongs to the IUNH family. RihC subfamily.
CC       {ECO:0000255|HAMAP-Rule:MF_01432}.
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DR   EMBL; AE005674; AAN41693.1; -; Genomic_DNA.
DR   EMBL; AE014073; AAP15574.1; -; Genomic_DNA.
DR   RefSeq; NP_705986.1; NC_004337.2.
DR   RefSeq; WP_001239124.1; NZ_WPGW01000005.1.
DR   AlphaFoldDB; Q83MH0; -.
DR   SMR; Q83MH0; -.
DR   STRING; 198214.SF0027; -.
DR   EnsemblBacteria; AAN41693; AAN41693; SF0027.
DR   EnsemblBacteria; AAP15574; AAP15574; S0029.
DR   GeneID; 1024590; -.
DR   GeneID; 58390367; -.
DR   KEGG; sfl:SF0027; -.
DR   KEGG; sfx:S0029; -.
DR   PATRIC; fig|198214.7.peg.30; -.
DR   HOGENOM; CLU_036838_2_2_6; -.
DR   OMA; TPVAEWN; -.
DR   OrthoDB; 1763387at2; -.
DR   Proteomes; UP000001006; Chromosome.
DR   Proteomes; UP000002673; Chromosome.
DR   GO; GO:0045437; F:uridine nucleosidase activity; IEA:UniProt.
DR   GO; GO:0006144; P:purine nucleobase metabolic process; IEA:UniProtKB-UniRule.
DR   GO; GO:0006206; P:pyrimidine nucleobase metabolic process; IEA:UniProtKB-UniRule.
DR   Gene3D; 3.90.245.10; -; 1.
DR   HAMAP; MF_01432; Nucleosid_hydro_RihC; 1.
DR   InterPro; IPR015910; I/U_nuclsd_hydro_CS.
DR   InterPro; IPR001910; Inosine/uridine_hydrolase_dom.
DR   InterPro; IPR023186; IUNH.
DR   InterPro; IPR022976; Nucleosid_hydro_RihC_nonspecif.
DR   InterPro; IPR036452; Ribo_hydro-like.
DR   PANTHER; PTHR12304; PTHR12304; 1.
DR   Pfam; PF01156; IU_nuc_hydro; 1.
DR   SUPFAM; SSF53590; SSF53590; 1.
DR   PROSITE; PS01247; IUNH; 1.
PE   3: Inferred from homology;
KW   Glycosidase; Hydrolase; Reference proteome.
FT   CHAIN           1..304
FT                   /note="Non-specific ribonucleoside hydrolase RihC"
FT                   /id="PRO_0000206838"
FT   ACT_SITE        233
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01432"
SQ   SEQUENCE   304 AA;  32609 MW;  5A8303638EBB5DBE CRC64;
     MRLPIFLDTD PGIDDAVAIA AAIFAPELDL QLMTTVAGNV SVEKTTRNAL QLLHFWNAEI
     PLAQGAAVPL VRAPRDAASV HGESGMAGYD FVEHNRKPLG IPAFLAIRDA LMRAPEPVTL
     VAIGPLTNIA LLLLQCPECK PYIRRLVIMG GSAGRGNCTP NAEFNIAADP EAAACVFRSG
     IEIVMCGLDV TNQAILTPDY LATLPELNRT GKMLHALFSH YRSGSMQSGL RMHDLCAIAW
     LVRPDLFTLK PCFVAVETQG EFTSGTTVVD IDGCLGKPAN VQVALDLNVK GFQQWVAEVL
     ALVP