RIH_DICDI
ID RIH_DICDI Reviewed; 340 AA.
AC Q7KWM9; Q558T2;
DT 08-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 03-AUG-2022, entry version 96.
DE RecName: Full=Probable ribonucleoside hydrolase {ECO:0000305};
DE EC=3.2.2.1 {ECO:0000305};
DE EC=3.2.2.8 {ECO:0000305};
GN Name=iunH; ORFNames=DDB_G0272738;
OS Dictyostelium discoideum (Slime mold).
OC Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC Dictyosteliaceae; Dictyostelium.
OX NCBI_TaxID=44689;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=12097910; DOI=10.1038/nature00847;
RA Gloeckner G., Eichinger L., Szafranski K., Pachebat J.A., Bankier A.T.,
RA Dear P.H., Lehmann R., Baumgart C., Parra G., Abril J.F., Guigo R.,
RA Kumpf K., Tunggal B., Cox E.C., Quail M.A., Platzer M., Rosenthal A.,
RA Noegel A.A.;
RT "Sequence and analysis of chromosome 2 of Dictyostelium discoideum.";
RL Nature 418:79-85(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=15875012; DOI=10.1038/nature03481;
RA Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT "The genome of the social amoeba Dictyostelium discoideum.";
RL Nature 435:43-57(2005).
CC -!- FUNCTION: Catalyzes the hydrolysis of the N-glycosidic bond of purine
CC and/or pyrimidine nucleosides into ribose and the base. {ECO:0000305}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a purine D-ribonucleoside + H2O = a purine nucleobase + D-
CC ribose; Xref=Rhea:RHEA:23344, ChEBI:CHEBI:15377, ChEBI:CHEBI:26386,
CC ChEBI:CHEBI:47013, ChEBI:CHEBI:142355; EC=3.2.2.1;
CC Evidence={ECO:0000305};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a pyrimidine ribonucleoside + H2O = a pyrimidine nucleobase +
CC D-ribose; Xref=Rhea:RHEA:56816, ChEBI:CHEBI:15377, ChEBI:CHEBI:26432,
CC ChEBI:CHEBI:47013, ChEBI:CHEBI:141014; EC=3.2.2.8;
CC Evidence={ECO:0000305};
CC -!- COFACTOR:
CC Name=Ca(2+); Xref=ChEBI:CHEBI:29108;
CC Evidence={ECO:0000250|UniProtKB:P83851};
CC -!- PATHWAY: Purine metabolism; purine nucleoside salvage.
CC -!- SIMILARITY: Belongs to the IUNH family. {ECO:0000305}.
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DR EMBL; AAFI02000008; EAL71006.1; -; Genomic_DNA.
DR RefSeq; XP_644984.1; XM_639892.1.
DR AlphaFoldDB; Q7KWM9; -.
DR SMR; Q7KWM9; -.
DR STRING; 44689.DDB0231227; -.
DR PaxDb; Q7KWM9; -.
DR EnsemblProtists; EAL71006; EAL71006; DDB_G0272738.
DR GeneID; 8618661; -.
DR KEGG; ddi:DDB_G0272738; -.
DR dictyBase; DDB_G0272738; iunH.
DR eggNOG; KOG2938; Eukaryota.
DR HOGENOM; CLU_036838_2_0_1; -.
DR InParanoid; Q7KWM9; -.
DR OMA; HAPDIHG; -.
DR PhylomeDB; Q7KWM9; -.
DR UniPathway; UPA00606; -.
DR PRO; PR:Q7KWM9; -.
DR Proteomes; UP000002195; Chromosome 2.
DR GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR GO; GO:0016798; F:hydrolase activity, acting on glycosyl bonds; ISS:dictyBase.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0008477; F:purine nucleosidase activity; IBA:GO_Central.
DR GO; GO:0050263; F:ribosylpyrimidine nucleosidase activity; IEA:UniProtKB-EC.
DR GO; GO:0009117; P:nucleotide metabolic process; IEA:UniProtKB-KW.
DR GO; GO:0006152; P:purine nucleoside catabolic process; IBA:GO_Central.
DR GO; GO:0008655; P:pyrimidine-containing compound salvage; ISS:dictyBase.
DR GO; GO:0006218; P:uridine catabolic process; ISS:dictyBase.
DR Gene3D; 3.90.245.10; -; 1.
DR InterPro; IPR001910; Inosine/uridine_hydrolase_dom.
DR InterPro; IPR023186; IUNH.
DR InterPro; IPR036452; Ribo_hydro-like.
DR PANTHER; PTHR12304; PTHR12304; 1.
DR Pfam; PF01156; IU_nuc_hydro; 1.
DR SUPFAM; SSF53590; SSF53590; 1.
PE 3: Inferred from homology;
KW Calcium; Glycosidase; Hydrolase; Metal-binding; Nucleotide metabolism;
KW Reference proteome.
FT CHAIN 1..340
FT /note="Probable ribonucleoside hydrolase"
FT /id="PRO_0000328284"
FT ACT_SITE 259
FT /note="Proton donor"
FT /evidence="ECO:0000250|UniProtKB:Q27546"
FT BINDING 14
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000250"
FT BINDING 18
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT BINDING 19
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000250"
FT BINDING 139
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000250"
FT BINDING 172
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT BINDING 178
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT BINDING 180
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT BINDING 260
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000250"
SQ SEQUENCE 340 AA; 37993 MW; 2C8D6346C51C687A CRC64;
MLNEELLPIW LDHDCGHDDA FAMLLAFHSK IFNILGISSV HGNQTVDKTT INALITLEII
GKSNCGYEVV KGVRSPMCRP EQVCSEIHGE TGLDCPTAEL PKPTQLPITD RPAIQVMFEK
ISKFYTDNQQ KQKVIIVATG SLTNVALLFA VYPQIKPMVE VSLLGGSINF GNISPAAEYN
ILVDPEAAKV VFESGVKVIM VPLECSHKAL VNEKILERIS DIEKADGKQT QFIKIIKGLL
LFFADNYKST FDFDHPPLHD PLAVAYLIDP TIFKCKLMRV DIETSSHLCL GRTVVDLFSM
SKLQKNVHVC TDINVDKFWD LMINAIDNCY NHLYKSNILK
