RIM1_YEAST
ID RIM1_YEAST Reviewed; 135 AA.
AC P32445; D6VR39; O11851;
DT 01-OCT-1993, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1993, sequence version 1.
DT 03-AUG-2022, entry version 180.
DE RecName: Full=Single-stranded DNA-binding protein RIM1, mitochondrial;
DE AltName: Full=Mitochondrial ssDNA-binding protein;
DE Flags: Precursor;
GN Name=RIM1; OrderedLocusNames=YCR028C-A; ORFNames=YCR28C-A;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], AND PARTIAL PROTEIN SEQUENCE.
RX PubMed=1324172;
RA van Dyck E., Foury F., Stillman B., Brill S.J.;
RT "A single-stranded DNA binding protein required for mitochondrial DNA
RT replication in S. cerevisiae is homologous to E. coli SSB.";
RL EMBO J. 11:3421-3430(1992).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204511 / S288c / AB972;
RX PubMed=1332309; DOI=10.1002/yea.320080915;
RA Carbone M.L.A., Panzeri L., Falconi M.M., Carcano C., Plevani P.,
RA Lucchini G.;
RT "Nucleotide sequence of 9.2 kb left of CRY1 on yeast chromosome III from
RT strain AB972: evidence for a Ty insertion and functional analysis of open
RT reading frame YCR28.";
RL Yeast 8:805-812(1992).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=1574125; DOI=10.1038/357038a0;
RA Oliver S.G., van der Aart Q.J.M., Agostoni-Carbone M.L., Aigle M.,
RA Alberghina L., Alexandraki D., Antoine G., Anwar R., Ballesta J.P.G.,
RA Benit P., Berben G., Bergantino E., Biteau N., Bolle P.-A.,
RA Bolotin-Fukuhara M., Brown A., Brown A.J.P., Buhler J.-M., Carcano C.,
RA Carignani G., Cederberg H., Chanet R., Contreras R., Crouzet M.,
RA Daignan-Fornier B., Defoor E., Delgado M.D., Demolder J., Doira C.,
RA Dubois E., Dujon B., Duesterhoeft A., Erdmann D., Esteban M., Fabre F.,
RA Fairhead C., Faye G., Feldmann H., Fiers W., Francingues-Gaillard M.-C.,
RA Franco L., Frontali L., Fukuhara H., Fuller L.J., Galland P., Gent M.E.,
RA Gigot D., Gilliquet V., Glansdorff N., Goffeau A., Grenson M., Grisanti P.,
RA Grivell L.A., de Haan M., Haasemann M., Hatat D., Hoenicka J.,
RA Hegemann J.H., Herbert C.J., Hilger F., Hohmann S., Hollenberg C.P.,
RA Huse K., Iborra F., Indge K.J., Isono K., Jacq C., Jacquet M., James C.M.,
RA Jauniaux J.-C., Jia Y., Jimenez A., Kelly A., Kleinhans U., Kreisl P.,
RA Lanfranchi G., Lewis C., van der Linden C.G., Lucchini G.,
RA Lutzenkirchen K., Maat M.J., Mallet L., Mannhaupt G., Martegani E.,
RA Mathieu A., Maurer C.T.C., McConnell D., McKee R.A., Messenguy F.,
RA Mewes H.-W., Molemans F., Montague M.A., Muzi Falconi M., Navas L.,
RA Newlon C.S., Noone D., Pallier C., Panzeri L., Pearson B.M., Perea J.,
RA Philippsen P., Pierard A., Planta R.J., Plevani P., Poetsch B., Pohl F.M.,
RA Purnelle B., Ramezani Rad M., Rasmussen S.W., Raynal A., Remacha M.A.,
RA Richterich P., Roberts A.B., Rodriguez F., Sanz E.,
RA Schaaff-Gerstenschlaeger I., Scherens B., Schweitzer B., Shu Y., Skala J.,
RA Slonimski P.P., Sor F., Soustelle C., Spiegelberg R., Stateva L.I.,
RA Steensma H.Y., Steiner S., Thierry A., Thireos G., Tzermia M.,
RA Urrestarazu L.A., Valle G., Vetter I., van Vliet-Reedijk J.C., Voet M.,
RA Volckaert G., Vreken P., Wang H., Warmington J.R., von Wettstein D.,
RA Wicksteed B.L., Wilson C., Wurst H., Xu G., Yoshikawa A., Zimmermann F.K.,
RA Sgouros J.G.;
RT "The complete DNA sequence of yeast chromosome III.";
RL Nature 357:38-46(1992).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [5]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
RN [6]
RP FUNCTION, INTERACTION WITH PIF1, AND SUBUNIT.
RX PubMed=23175612; DOI=10.1093/nar/gks1088;
RA Ramanagoudr-Bhojappa R., Blair L.P., Tackett A.J., Raney K.D.;
RT "Physical and functional interaction between yeast Pif1 helicase and Rim1
RT single-stranded DNA binding protein.";
RL Nucleic Acids Res. 41:1029-1046(2013).
CC -!- FUNCTION: This protein binds preferentially and cooperatively to
CC single-stranded DNA (ssDNS). Involved in mitochondrial DNA replication.
CC Stimulates PIF1 helicase activity. {ECO:0000269|PubMed:23175612}.
CC -!- SUBUNIT: Homotetramer. Interacts with PIF1.
CC {ECO:0000269|PubMed:23175612}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion.
CC -!- MISCELLANEOUS: Present with 3060 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
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DR EMBL; S43128; AAB22978.1; -; Genomic_DNA.
DR EMBL; S43129; AAD13828.1; -; mRNA.
DR EMBL; X59720; CAA42321.2; -; Genomic_DNA.
DR EMBL; BK006937; DAA07508.1; -; Genomic_DNA.
DR PIR; S23548; S23548.
DR RefSeq; NP_009958.2; NM_001184315.1.
DR PDB; 6CQK; X-ray; 2.80 A; A/B/C/D=17-135.
DR PDB; 6CQM; X-ray; 3.00 A; A/B/C/D/E/F/G/H=17-135.
DR PDB; 6CQO; X-ray; 2.80 A; A/B/C/D/E/F/G/H=17-135.
DR PDBsum; 6CQK; -.
DR PDBsum; 6CQM; -.
DR PDBsum; 6CQO; -.
DR AlphaFoldDB; P32445; -.
DR SMR; P32445; -.
DR BioGRID; 31012; 423.
DR DIP; DIP-7376N; -.
DR IntAct; P32445; 46.
DR MINT; P32445; -.
DR STRING; 4932.YCR028C-A; -.
DR iPTMnet; P32445; -.
DR MaxQB; P32445; -.
DR PaxDb; P32445; -.
DR PRIDE; P32445; -.
DR TopDownProteomics; P32445; -.
DR EnsemblFungi; YCR028C-A_mRNA; YCR028C-A; YCR028C-A.
DR GeneID; 850395; -.
DR KEGG; sce:YCR028C-A; -.
DR SGD; S000007222; RIM1.
DR VEuPathDB; FungiDB:YCR028C-A; -.
DR eggNOG; ENOG502S0M8; Eukaryota.
DR HOGENOM; CLU_126647_1_1_1; -.
DR InParanoid; P32445; -.
DR OMA; QYVRKGA; -.
DR BioCyc; YEAST:G3O-29415-MON; -.
DR PRO; PR:P32445; -.
DR Proteomes; UP000002311; Chromosome III.
DR RNAct; P32445; protein.
DR GO; GO:0042645; C:mitochondrial nucleoid; IDA:SGD.
DR GO; GO:0005739; C:mitochondrion; IDA:SGD.
DR GO; GO:0009295; C:nucleoid; IBA:GO_Central.
DR GO; GO:0003697; F:single-stranded DNA binding; IDA:SGD.
DR GO; GO:0006264; P:mitochondrial DNA replication; ISS:SGD.
DR GO; GO:0000002; P:mitochondrial genome maintenance; IMP:SGD.
DR GO; GO:0051096; P:positive regulation of helicase activity; IBA:GO_Central.
DR GO; GO:0090297; P:positive regulation of mitochondrial DNA replication; IDA:SGD.
DR CDD; cd04496; SSB_OBF; 1.
DR Gene3D; 2.40.50.140; -; 1.
DR InterPro; IPR012340; NA-bd_OB-fold.
DR InterPro; IPR000424; Primosome_PriB/ssb.
DR InterPro; IPR011344; ssDNA-bd.
DR Pfam; PF00436; SSB; 1.
DR PIRSF; PIRSF002070; SSB; 1.
DR SUPFAM; SSF50249; SSF50249; 1.
DR PROSITE; PS50935; SSB; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Direct protein sequencing; DNA replication; DNA-binding;
KW Mitochondrion; Reference proteome; Transit peptide.
FT TRANSIT 1..17
FT /note="Mitochondrion"
FT CHAIN 18..135
FT /note="Single-stranded DNA-binding protein RIM1,
FT mitochondrial"
FT /id="PRO_0000033262"
FT DOMAIN 19..117
FT /note="SSB"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00252"
FT STRAND 19..28
FT /evidence="ECO:0007829|PDB:6CQK"
FT STRAND 33..36
FT /evidence="ECO:0007829|PDB:6CQK"
FT STRAND 38..40
FT /evidence="ECO:0007829|PDB:6CQK"
FT STRAND 42..50
FT /evidence="ECO:0007829|PDB:6CQK"
FT STRAND 52..56
FT /evidence="ECO:0007829|PDB:6CQK"
FT STRAND 59..65
FT /evidence="ECO:0007829|PDB:6CQK"
FT HELIX 68..75
FT /evidence="ECO:0007829|PDB:6CQK"
FT STRAND 83..93
FT /evidence="ECO:0007829|PDB:6CQK"
FT STRAND 105..117
FT /evidence="ECO:0007829|PDB:6CQK"
SQ SEQUENCE 135 AA; 15386 MW; 9F913C2FC9EF6E82 CRC64;
MFLRTQARFF HATTKKMDFS KMSIVGRIGS EFTEHTSANN NRYLKYSIAS QPRRDGQTNW
YNITVFNEPQ INFLTEYVRK GALVYVEADA ANYVFERDDG SKGTTLSLVQ KDINLLKNGK
KLEDAEGQEN AASSE
