RIM2_DROME
ID RIM2_DROME Reviewed; 365 AA.
AC Q9VQ37; Q95TJ5;
DT 12-APR-2017, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2002, sequence version 2.
DT 03-AUG-2022, entry version 166.
DE RecName: Full=Mitochondrial carrier protein Rim2 {ECO:0000303|PubMed:24469456};
GN Name=Rim2 {ECO:0000303|PubMed:24469456, ECO:0000312|FlyBase:FBgn0031359};
GN ORFNames=CG18317 {ECO:0000312|FlyBase:FBgn0031359};
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227 {ECO:0000312|Proteomes:UP000000803};
RN [1] {ECO:0000312|Proteomes:UP000000803}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley {ECO:0000312|Proteomes:UP000000803};
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2] {ECO:0000312|Proteomes:UP000000803}
RP GENOME REANNOTATION.
RC STRAIN=Berkeley {ECO:0000312|Proteomes:UP000000803};
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3] {ECO:0000312|EMBL:AAL13964.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley {ECO:0000312|EMBL:AAL13964.1};
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [4] {ECO:0000305}
RP FUNCTION, SUBCELLULAR LOCATION, AND DISRUPTION PHENOTYPE.
RX PubMed=24469456; DOI=10.1074/jbc.m113.543926;
RA Da-Re C., Franzolin E., Biscontin A., Piazzesi A., Pacchioni B.,
RA Gagliani M.C., Mazzotta G., Tacchetti C., Zordan M.A., Zeviani M.,
RA Bernardi P., Bianchi V., De Pitta C., Costa R.;
RT "Functional characterization of drim2, the Drosophila melanogaster homolog
RT of the yeast mitochondrial deoxynucleotide transporter.";
RL J. Biol. Chem. 289:7448-7459(2014).
CC -!- FUNCTION: Mitochondrial transporter that imports purine and pyrimidine
CC nucleotides into the mitochondria. Essential for maintaining
CC mitochondrial structure and function. Appears to be important for
CC mitochondrial gene transcription and mitochondrial respiration.
CC {ECO:0000269|PubMed:24469456}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion {ECO:0000269|PubMed:24469456}.
CC Mitochondrion inner membrane {ECO:0000305|PubMed:24469456}; Multi-pass
CC membrane protein {ECO:0000305}.
CC -!- DISRUPTION PHENOTYPE: Larval lethal at the third instar stage. Third-
CC instar larva are decreased in size and display reduced locomotion
CC activity. In third stage larvae body wall preparations, mitochondria
CC are abnormally shaped, disorganized and have a significantly higher
CC density. Oxygen consumption rate (i.e. mitochondrial respiration) is
CC also significantly lower than the controls.
CC {ECO:0000269|PubMed:24469456}.
CC -!- SIMILARITY: Belongs to the mitochondrial carrier (TC 2.A.29) family.
CC {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AE014134; AAF51345.2; -; Genomic_DNA.
DR EMBL; AY058735; AAL13964.1; -; mRNA.
DR RefSeq; NP_608615.1; NM_134771.4.
DR AlphaFoldDB; Q9VQ37; -.
DR SMR; Q9VQ37; -.
DR STRING; 7227.FBpp0077576; -.
DR PaxDb; Q9VQ37; -.
DR DNASU; 33350; -.
DR EnsemblMetazoa; FBtr0077910; FBpp0077576; FBgn0031359.
DR GeneID; 33350; -.
DR KEGG; dme:Dmel_CG18317; -.
DR UCSC; CG18317-RA; d. melanogaster.
DR CTD; 33350; -.
DR FlyBase; FBgn0031359; Rim2.
DR VEuPathDB; VectorBase:FBgn0031359; -.
DR eggNOG; KOG0757; Eukaryota.
DR GeneTree; ENSGT00940000168859; -.
DR InParanoid; Q9VQ37; -.
DR PhylomeDB; Q9VQ37; -.
DR BioGRID-ORCS; 33350; 0 hits in 3 CRISPR screens.
DR GenomeRNAi; 33350; -.
DR PRO; PR:Q9VQ37; -.
DR Proteomes; UP000000803; Chromosome 2L.
DR Bgee; FBgn0031359; Expressed in brain and 29 other tissues.
DR ExpressionAtlas; Q9VQ37; baseline and differential.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005743; C:mitochondrial inner membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005739; C:mitochondrion; IDA:FlyBase.
DR GO; GO:0015218; F:pyrimidine nucleotide transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0045333; P:cellular respiration; IMP:FlyBase.
DR GO; GO:0030302; P:deoxynucleotide transport; IMP:FlyBase.
DR GO; GO:0040011; P:locomotion; IMP:FlyBase.
DR GO; GO:0000002; P:mitochondrial genome maintenance; IBA:GO_Central.
DR GO; GO:0006390; P:mitochondrial transcription; IMP:FlyBase.
DR GO; GO:0006839; P:mitochondrial transport; IEA:InterPro.
DR GO; GO:0048311; P:mitochondrion distribution; IMP:FlyBase.
DR GO; GO:0007005; P:mitochondrion organization; IMP:FlyBase.
DR GO; GO:1990519; P:pyrimidine nucleotide import into mitochondrion; IBA:GO_Central.
DR Gene3D; 1.50.40.10; -; 2.
DR InterPro; IPR002030; Mit_uncoupling_UCP-like.
DR InterPro; IPR018108; Mitochondrial_sb/sol_carrier.
DR InterPro; IPR023395; Mt_carrier_dom_sf.
DR Pfam; PF00153; Mito_carr; 3.
DR PRINTS; PR00784; MTUNCOUPLING.
DR SUPFAM; SSF103506; SSF103506; 1.
DR PROSITE; PS50920; SOLCAR; 3.
PE 2: Evidence at transcript level;
KW Membrane; Mitochondrion; Mitochondrion inner membrane; Reference proteome;
KW Repeat; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..365
FT /note="Mitochondrial carrier protein Rim2"
FT /id="PRO_0000439452"
FT TRANSMEM 9..29
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 171..191
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 227..247
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 268..288
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REPEAT 6..155
FT /note="Solcar 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00282"
FT REPEAT 165..250
FT /note="Solcar 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00282"
FT REPEAT 266..350
FT /note="Solcar 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00282"
SQ SEQUENCE 365 AA; 40476 MW; 81DA658CBA508642 CRC64;
MAQNTADTLI HLIAGGSAGT VGAVVTCPLE VVKTRLQSST AFMTPSRLAE NAGGGPANGG
QSELLRPEQR RKLSTTILRN RSQPQVIGGV RRIMAISHCG ISSTTPKSMS IVQCLRHIVQ
NEGPRALFKG LGPNLVGVAP SRAIYFCTYS QTKNTLNSLG FVERDSPLVH IMSAASAGFV
SSTATNPIWF VKTRMQLDYN SKVQMTVRQC IERVYAQGGV AAFYKGITAS YFGICETMVH
FVIYEFIKSK LLEQRNQRHT DTKGSRDFLE FMMAGAVSKT IASCIAYPHE VARTRLREEG
NKYNSFWQTL HTVWKEEGRA GLYRGLATQL VRQIPNTAIM MATYEAVVYV LTRRFNNKSN
EFYDF
