RIMP_ECOLI
ID RIMP_ECOLI Reviewed; 150 AA.
AC P0A8A8; P03843; Q2M940; Q8X9M1;
DT 21-JUL-1986, integrated into UniProtKB/Swiss-Prot.
DT 24-NOV-2009, sequence version 2.
DT 03-AUG-2022, entry version 119.
DE RecName: Full=Ribosome maturation factor RimP;
GN Name=rimP; Synonyms=yhbC; OrderedLocusNames=b3170, JW5533;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=6326058; DOI=10.1093/nar/12.7.3333;
RA Ishii S., Ihara M., Maekawa T., Nakamura Y., Uchida H., Imamoto F.;
RT "The nucleotide sequence of the cloned nusA gene and its flanking region of
RT Escherichia coli.";
RL Nucleic Acids Res. 12:3333-3342(1984).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [4]
RP FUNCTION, BINDING TO 30S SUBUNITS, AND DISRUPTION PHENOTYPE.
RX PubMed=19150615; DOI=10.1016/j.jmb.2008.12.076;
RA Nord S., Bylund G.O., Loevgren J.M., Wikstroem P.M.;
RT "The RimP protein is important for maturation of the 30S ribosomal
RT subunit.";
RL J. Mol. Biol. 386:742-753(2009).
RN [5]
RP FUNCTION IN 30S SUBUNIT PROTEIN ASSEMBLY.
RX PubMed=20188109; DOI=10.1016/j.jmb.2010.02.036;
RA Bunner A.E., Nord S., Wikstrom P.M., Williamson J.R.;
RT "The effect of ribosome assembly cofactors on in vitro 30S subunit
RT reconstitution.";
RL J. Mol. Biol. 398:1-7(2010).
CC -!- FUNCTION: Required for maturation of 30S ribosomal subunits, probably
CC at a late stage of ribosomal protein binding, while Era is associated
CC and after RimM (PubMed:20188109). {ECO:0000269|PubMed:19150615,
CC ECO:0000269|PubMed:20188109}.
CC -!- SUBUNIT: Associates with free 30S subunits, but not with free 50S
CC subunits or with mature ribosomes.
CC -!- INTERACTION:
CC P0A8A8; P0A7S3: rpsL; NbExp=3; IntAct=EBI-561065, EBI-543960;
CC P0A8A8; P0A8H6: yihI; NbExp=2; IntAct=EBI-561065, EBI-552497;
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
CC -!- DISRUPTION PHENOTYPE: Cells lacking this gene show a reduced growth
CC rate, especially at high temperatures. They show a reduced amount of
CC 70S ribosomes engaged in translation and a corresponding increase in
CC the amount of free ribosomal subunits. {ECO:0000269|PubMed:19150615}.
CC -!- SIMILARITY: Belongs to the RimP family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAA57973.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
CC Sequence=CAA25199.1; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; X00513; CAA25199.1; ALT_FRAME; Genomic_DNA.
DR EMBL; U18997; AAA57973.1; ALT_INIT; Genomic_DNA.
DR EMBL; U00096; AAC76204.4; -; Genomic_DNA.
DR EMBL; AP009048; BAE77216.1; -; Genomic_DNA.
DR PIR; F65107; WMECN5.
DR RefSeq; NP_417639.6; NC_000913.3.
DR RefSeq; WP_001300397.1; NZ_STEB01000012.1.
DR PDB; 7AFI; EM; 3.53 A; X=1-150.
DR PDB; 7AFL; EM; 4.20 A; X=1-150.
DR PDB; 7AFO; EM; 3.93 A; X=1-150.
DR PDB; 7AFR; NMR; -; X=1-150.
DR PDB; 7NAS; EM; 3.31 A; X=1-150.
DR PDB; 7NAT; EM; 3.59 A; X=1-150.
DR PDB; 7NAU; EM; 3.78 A; X=1-150.
DR PDB; 7NAV; EM; 4.80 A; X=1-150.
DR PDBsum; 7AFI; -.
DR PDBsum; 7AFL; -.
DR PDBsum; 7AFO; -.
DR PDBsum; 7AFR; -.
DR PDBsum; 7NAS; -.
DR PDBsum; 7NAT; -.
DR PDBsum; 7NAU; -.
DR PDBsum; 7NAV; -.
DR AlphaFoldDB; P0A8A8; -.
DR SMR; P0A8A8; -.
DR BioGRID; 4260791; 67.
DR BioGRID; 851993; 3.
DR DIP; DIP-47892N; -.
DR IntAct; P0A8A8; 7.
DR STRING; 511145.b3170; -.
DR jPOST; P0A8A8; -.
DR PaxDb; P0A8A8; -.
DR PRIDE; P0A8A8; -.
DR EnsemblBacteria; AAC76204; AAC76204; b3170.
DR EnsemblBacteria; BAE77216; BAE77216; BAE77216.
DR GeneID; 67414892; -.
DR GeneID; 947680; -.
DR KEGG; ecj:JW5533; -.
DR KEGG; eco:b3170; -.
DR PATRIC; fig|1411691.4.peg.3560; -.
DR EchoBASE; EB1166; -.
DR eggNOG; COG0779; Bacteria.
DR HOGENOM; CLU_070525_1_1_6; -.
DR InParanoid; P0A8A8; -.
DR OMA; YIHVSLY; -.
DR PhylomeDB; P0A8A8; -.
DR BioCyc; EcoCyc:EG11179-MON; -.
DR PRO; PR:P0A8A8; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0005829; C:cytosol; IDA:EcoCyc.
DR GO; GO:0000028; P:ribosomal small subunit assembly; IMP:EcoCyc.
DR GO; GO:0042274; P:ribosomal small subunit biogenesis; IMP:EcoliWiki.
DR GO; GO:0006412; P:translation; IMP:EcoliWiki.
DR CDD; cd01734; YlxS_C; 1.
DR Gene3D; 3.30.300.70; -; 1.
DR HAMAP; MF_01077; RimP; 1.
DR InterPro; IPR003728; Ribosome_maturation_RimP.
DR InterPro; IPR028998; RimP_C.
DR InterPro; IPR036847; RimP_C_sf.
DR InterPro; IPR028989; RimP_N.
DR InterPro; IPR035956; RimP_N_sf.
DR PANTHER; PTHR33867; PTHR33867; 1.
DR Pfam; PF02576; DUF150; 1.
DR Pfam; PF17384; DUF150_C; 1.
DR SUPFAM; SSF74942; SSF74942; 1.
DR SUPFAM; SSF75420; SSF75420; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Cytoplasm; Reference proteome; Ribosome biogenesis.
FT CHAIN 1..150
FT /note="Ribosome maturation factor RimP"
FT /id="PRO_0000181869"
FT CONFLICT 9
FT /note="T -> A (in Ref. 1; CAA25199)"
FT /evidence="ECO:0000305"
FT HELIX 5..18
FT /evidence="ECO:0007829|PDB:7NAS"
FT STRAND 22..29
FT /evidence="ECO:0007829|PDB:7NAS"
FT STRAND 35..41
FT /evidence="ECO:0007829|PDB:7NAS"
FT HELIX 48..64
FT /evidence="ECO:0007829|PDB:7NAS"
FT STRAND 74..77
FT /evidence="ECO:0007829|PDB:7NAS"
FT STRAND 81..84
FT /evidence="ECO:0007829|PDB:7NAS"
FT HELIX 88..93
FT /evidence="ECO:0007829|PDB:7NAS"
FT STRAND 96..104
FT /evidence="ECO:0007829|PDB:7NAS"
FT STRAND 113..120
FT /evidence="ECO:0007829|PDB:7NAS"
FT STRAND 122..129
FT /evidence="ECO:0007829|PDB:7NAS"
FT STRAND 132..137
FT /evidence="ECO:0007829|PDB:7NAS"
FT HELIX 138..140
FT /evidence="ECO:0007829|PDB:7NAS"
FT STRAND 141..146
FT /evidence="ECO:0007829|PDB:7NAS"
SQ SEQUENCE 150 AA; 16651 MW; FFEC71975B4492AB CRC64;
MSTLEQKLTE MITAPVEALG FELVGIEFIR GRTSTLRIYI DSEDGINVDD CADVSHQVSA
VLDVEDPITV AYNLEVSSPG LDRPLFTAEH YARFVGEEVT LVLRMAVQNR RKWQGVIKAV
DGEMITVTVE GKDEVFALSN IQKANLVPHF
