RIN3_MOUSE
ID RIN3_MOUSE Reviewed; 980 AA.
AC P59729; B2RQF8;
DT 20-JUN-2003, integrated into UniProtKB/Swiss-Prot.
DT 27-JUL-2011, sequence version 2.
DT 03-AUG-2022, entry version 132.
DE RecName: Full=Ras and Rab interactor 3;
DE AltName: Full=Ras interaction/interference protein 3;
GN Name=Rin3;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Olfactory bulb;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Heart, Liver, Lung, and Spleen;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Ras effector protein that functions as a guanine nucleotide
CC exchange (GEF) for RAB5B and RAB31, by exchanging bound GDP for free
CC GTP. Required for normal RAB31 function (By similarity). {ECO:0000250}.
CC -!- SUBUNIT: Interacts with CD2AP, RAB5B, RAB31 and BIN1. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:Q8TB24}.
CC Cytoplasmic vesicle {ECO:0000250|UniProtKB:Q8TB24}. Early endosome
CC {ECO:0000250|UniProtKB:Q8TB24}. Note=Activation of tyrosine
CC phosphorylation signaling induces translocation to cytoplasmic
CC vesicles. {ECO:0000250|UniProtKB:Q8TB24}.
CC -!- SIMILARITY: Belongs to the RIN (Ras interaction/interference) family.
CC {ECO:0000305}.
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DR EMBL; AK032205; BAC27757.1; -; mRNA.
DR EMBL; BC137907; AAI37908.1; -; mRNA.
DR CCDS; CCDS26118.1; -.
DR RefSeq; NP_808288.2; NM_177620.4.
DR AlphaFoldDB; P59729; -.
DR SMR; P59729; -.
DR BioGRID; 229966; 5.
DR STRING; 10090.ENSMUSP00000060771; -.
DR iPTMnet; P59729; -.
DR PhosphoSitePlus; P59729; -.
DR EPD; P59729; -.
DR jPOST; P59729; -.
DR MaxQB; P59729; -.
DR PaxDb; P59729; -.
DR PRIDE; P59729; -.
DR ProteomicsDB; 254884; -.
DR Antibodypedia; 26773; 89 antibodies from 23 providers.
DR DNASU; 217835; -.
DR Ensembl; ENSMUST00000056950; ENSMUSP00000060771; ENSMUSG00000044456.
DR GeneID; 217835; -.
DR KEGG; mmu:217835; -.
DR UCSC; uc007ouc.2; mouse.
DR CTD; 79890; -.
DR MGI; MGI:2385708; Rin3.
DR VEuPathDB; HostDB:ENSMUSG00000044456; -.
DR eggNOG; KOG2320; Eukaryota.
DR GeneTree; ENSGT00940000158622; -.
DR InParanoid; P59729; -.
DR OMA; YCFVYHP; -.
DR OrthoDB; 251004at2759; -.
DR PhylomeDB; P59729; -.
DR TreeFam; TF331067; -.
DR Reactome; R-MMU-8876198; RAB GEFs exchange GTP for GDP on RABs.
DR BioGRID-ORCS; 217835; 2 hits in 70 CRISPR screens.
DR ChiTaRS; Rin3; mouse.
DR PRO; PR:P59729; -.
DR Proteomes; UP000000589; Chromosome 12.
DR RNAct; P59729; protein.
DR Bgee; ENSMUSG00000044456; Expressed in granulocyte and 146 other tissues.
DR ExpressionAtlas; P59729; baseline and differential.
DR Genevisible; P59729; MM.
DR GO; GO:0030424; C:axon; IDA:ARUK-UCL.
DR GO; GO:0005737; C:cytoplasm; ISO:MGI.
DR GO; GO:0031410; C:cytoplasmic vesicle; ISS:UniProtKB.
DR GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR GO; GO:0030425; C:dendrite; IDA:ARUK-UCL.
DR GO; GO:0005769; C:early endosome; ISS:UniProtKB.
DR GO; GO:0030139; C:endocytic vesicle; ISO:MGI.
DR GO; GO:0043025; C:neuronal cell body; IDA:ARUK-UCL.
DR GO; GO:0031982; C:vesicle; ISO:MGI.
DR GO; GO:0005096; F:GTPase activator activity; IEA:UniProtKB-KW.
DR GO; GO:0005085; F:guanyl-nucleotide exchange factor activity; ISS:UniProtKB.
DR GO; GO:0031267; F:small GTPase binding; ISS:UniProtKB.
DR GO; GO:0060755; P:negative regulation of mast cell chemotaxis; ISO:MGI.
DR GO; GO:0002091; P:negative regulation of receptor internalization; ISO:MGI.
DR GO; GO:0097494; P:regulation of vesicle size; ISO:MGI.
DR GO; GO:0007165; P:signal transduction; IEA:InterPro.
DR GO; GO:0016192; P:vesicle-mediated transport; IEA:InterPro.
DR CDD; cd10395; SH2_RIN3; 1.
DR Gene3D; 1.20.1050.80; -; 1.
DR Gene3D; 3.30.505.10; -; 1.
DR InterPro; IPR000159; RA_dom.
DR InterPro; IPR035869; RIN3_SH2.
DR InterPro; IPR036860; SH2_dom_sf.
DR InterPro; IPR003123; VPS9.
DR InterPro; IPR045046; Vps9-like.
DR InterPro; IPR037191; VPS9_dom_sf.
DR PANTHER; PTHR23101; PTHR23101; 1.
DR Pfam; PF02204; VPS9; 1.
DR SMART; SM00314; RA; 1.
DR SMART; SM00167; VPS9; 1.
DR SUPFAM; SSF109993; SSF109993; 1.
DR SUPFAM; SSF55550; SSF55550; 1.
DR PROSITE; PS50200; RA; 1.
DR PROSITE; PS51205; VPS9; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; Cytoplasmic vesicle; Endosome; GTPase activation;
KW Reference proteome; SH2 domain.
FT CHAIN 1..980
FT /note="Ras and Rab interactor 3"
FT /id="PRO_0000191323"
FT DOMAIN 63..158
FT /note="SH2"
FT DOMAIN 700..843
FT /note="VPS9"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00550"
FT DOMAIN 865..962
FT /note="Ras-associating"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00166"
FT REGION 1..40
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 247..496
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 509..560
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 584..729
FT /note="Interaction with RAB5B"
FT /evidence="ECO:0000250"
FT COMPBIAS 20..35
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 247..262
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 271..307
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 359..375
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 389..430
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 470..495
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 509..558
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 288
FT /note="T -> I (in Ref. 1; BAC27757)"
FT /evidence="ECO:0000305"
FT CONFLICT 332
FT /note="T -> K (in Ref. 1; BAC27757)"
FT /evidence="ECO:0000305"
FT CONFLICT 560
FT /note="S -> T (in Ref. 1; BAC27757)"
FT /evidence="ECO:0000305"
FT CONFLICT 563..565
FT /note="NVK -> DVE (in Ref. 1; BAC27757)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 980 AA; 107220 MW; F803A8CFEE17A7B8 CRC64;
MRRAEAPSSA HPAGPIPDAG KGEGEEDEEK DGTRLGLSTT PRNCIPRRGI SVLEKLVKTC
PVWLQLGLGQ AEAAKILQQE MAGMFLVCRD NNLKQLVLCV HFPSLKGSSA EVLEYPIKEE
KAILYLEGSV LVFEDIFRLI AFYCVSRDLL PFTLRLPQAI LEASSFLELE TISNLGLGFW
DSSLNSRGSA EPLRSPAPGT PASSSLRPTT HYANCSCEIE LSVGNDRLWF VNPIFIEDCI
LPADPPPLPT GSYPPRPTPA TPDATSPTSK GSPRRPPPPP PLPTVPPTGP ARPLAPPVPP
AGPLPNSPLT PTSHLAPHAP GPPGHSNQPP MTACESLPRP AVGLGPFGEE EMKPGTTPNP
LHQAPPPPLP LKKALPAAPP RRRISERVSL ESQNVGTSTD RDHSGISRTA SLNLPPQSTV
SSLGDRPPRT TEQSQDTEAK ASHADSIPVP PGKAKQPPVP PPRKKRVSRQ LASTLLSPLE
SPIQEASSEK QATGASWEGL SPVRQAGMQH LQVQSSSCPQ SSPEFKGSQA SLSDSLGVPA
SAADQDSYST SSAEEELEFS SPNVKKKPSM ILDKARHRLS FVSFASVFHA FLSSDRKLYK
KVVELAQDKS SYFGSLVQDY KVYSLEMMAR QTSSTEMLQE IRTMMTQLKS YLLQSTELKA
LVEPTLHSEE ELEAIVESAL YKCVLKPLKE AINSSLLEIH SRDGSLQQLK ENQLVVLATT
TTDLGVTTSV PEVAVMEKIL QKLTSMHKAY SPGKKISILL KTCKLIYDSM ALGNPGKPYG
ADDFLPVLMY VLARSNLTEM LLNVEYMMEL MDPALQLGEG SYYLTTTYGA LEHIKNYDKI
TVTRQLSVEV QDSIHRWERR RTLNKARASR SSVQDFICVS YLKPEQQSRT LASRADTAAQ
ALCAQCAEKF EVSQPQDYRL FVLVDGRCFQ LADEALPHRI KGYLLRSEPK RDFHFVYRPQ
DSGKDASSQP CIVVREPNFL
