ID   RIP1_CUCFI              Reviewed;         286 AA.
AC   Q9FRX4;
DT   01-JUN-2001, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2001, sequence version 1.
DT   25-MAY-2022, entry version 70.
DE   RecName: Full=Putative ribosome-inactivating protein;
DE            EC=3.2.2.22;
DE   AltName: Full=rRNA N-glycosidase;
DE   Flags: Precursor;
OS   Cucumis ficifolius (Cucumis figarei).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; fabids; Cucurbitales; Cucurbitaceae; Benincaseae; Cucumis.
OX   NCBI_TaxID=131071;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RA   Yamada T., Ohki S.T., Osaki T.;
RT   "Cloning and analysis of a cDNA coding a putative ribosome-inactivating
RT   protein from Cucumis figarei.";
RL   Plant Biotechnol. 17:337-340(2000).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Endohydrolysis of the N-glycosidic bond at one specific
CC         adenosine on the 28S rRNA.; EC=3.2.2.22;
CC   -!- SIMILARITY: Belongs to the ribosome-inactivating protein family. Type 1
CC       RIP subfamily. {ECO:0000305}.
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DR   EMBL; AB045560; BAB19677.1; -; mRNA.
DR   AlphaFoldDB; Q9FRX4; -.
DR   SMR; Q9FRX4; -.
DR   GO; GO:0030598; F:rRNA N-glycosylase activity; IEA:UniProtKB-EC.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   GO; GO:0051607; P:defense response to virus; IEA:UniProtKB-KW.
DR   GO; GO:0017148; P:negative regulation of translation; IEA:UniProtKB-KW.
DR   Gene3D; 3.40.420.10; -; 1.
DR   Gene3D; 4.10.470.10; -; 1.
DR   InterPro; IPR036041; Ribosome-inact_prot_sf.
DR   InterPro; IPR017989; Ribosome_inactivat_1/2.
DR   InterPro; IPR001574; Ribosome_inactivat_prot.
DR   InterPro; IPR017988; Ribosome_inactivat_prot_CS.
DR   InterPro; IPR016138; Ribosome_inactivat_prot_sub1.
DR   InterPro; IPR016139; Ribosome_inactivat_prot_sub2.
DR   PANTHER; PTHR33453; PTHR33453; 1.
DR   Pfam; PF00161; RIP; 1.
DR   PRINTS; PR00396; SHIGARICIN.
DR   SUPFAM; SSF56371; SSF56371; 1.
DR   PROSITE; PS00275; SHIGA_RICIN; 1.
PE   2: Evidence at transcript level;
KW   Antiviral defense; Glycoprotein; Hydrolase; Plant defense;
KW   Protein synthesis inhibitor; Signal; Toxin.
FT   SIGNAL          1..21
FT                   /evidence="ECO:0000255"
FT   CHAIN           22..286
FT                   /note="Putative ribosome-inactivating protein"
FT                   /id="PRO_0000030757"
FT   ACT_SITE        185
FT                   /evidence="ECO:0000250"
FT   CARBOHYD        103
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        110
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        252
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   286 AA;  31772 MW;  4EFD4966E604DA41 CRC64;
     MNRFSVLMCL VILSIFHGVP TAEGDVTVKF SLLGSNHKSY SKFITSMRNA LPNAGDIYNI
     PLLVPSISGS RRYILMQLSN YEGNTITMAV DVTNVYIMGY LVNGTSYFFN ETDAQLASKF
     VFQGTKSITL PYSGNYQKLQ SVARKERDSI PLGFMALDSA ISTLYYYDSR SAPIAFLVLI
     QTTAEAARYK YIEKQIIDRI SVSKVPDLAA ISLENEWSLL SKQIQIAKSN NGQFQTPVKI
     INDKGILTEV TNVSSLVVTK NIMLLLNKLN IASFEDHVIS TTMPQA