RIP1_GYNPE
ID RIP1_GYNPE Reviewed; 300 AA.
AC Q5F0I3; P83206; Q0PY48; Q7Y1U4; Q7Y1U5; Q7Y1U6; Q7Y1U7; Q84JR1; Q84LJ1;
AC Q8GV09; Q8GV10; Q8GV11; Q8H1Y4; Q8H1Y5; Q8RY69;
DT 09-JAN-2007, integrated into UniProtKB/Swiss-Prot.
DT 09-JAN-2007, sequence version 2.
DT 25-MAY-2022, entry version 54.
DE RecName: Full=Ribosome-inactivating protein gynostemmin;
DE EC=3.2.2.22;
DE AltName: Full=rRNA N-glycosidase;
DE Flags: Precursor;
OS Gynostemma pentaphyllum (Jiaogulan) (Sweet tea vine).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; fabids; Cucurbitales; Cucurbitaceae; Gomphogyneae; Gynostemma.
OX NCBI_TaxID=182084;
RN [1] {ECO:0000305, ECO:0000312|EMBL:AAS15568.1}
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
RA Lin Y., Wu Z.J., Xie L.H., Lin Q.Y.;
RT "Gynostemmin, full-length sequence with one ORF encoding ribosome-
RT inactivating protein from Gynostemma pentaphyllum (Thunb.) Makino.";
RL Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000305, ECO:0000312|EMBL:AAS15568.1}
RP PROTEIN SEQUENCE OF 24-42.
RC TISSUE=Leaf, and Stem;
RA Lin Y., Wu Z.J., Lin Q.Y., Xie L.H.;
RL Submitted (DEC-2001) to UniProtKB.
RN [3] {ECO:0000305, ECO:0000312|EMBL:AAS15568.1}
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 31-300.
RA Xiang T., Xu J.;
RT "A gene encoding ribosome-inactivating protein from Gynostemma
RT pentaphyllum.";
RL Submitted (MAY-2006) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Ribosome-inactivating protein of type 1, inhibits protein
CC synthesis in animal cells. {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Endohydrolysis of the N-glycosidic bond at one specific
CC adenosine on the 28S rRNA.; EC=3.2.2.22;
CC Evidence={ECO:0000250|UniProtKB:P16094};
CC -!- SIMILARITY: Belongs to the ribosome-inactivating protein family. Type 1
CC RIP subfamily. {ECO:0000255}.
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DR EMBL; AY075115; AAL82822.2; -; Genomic_DNA.
DR EMBL; AY134616; AAN08103.1; -; mRNA.
DR EMBL; AY134617; AAN08104.1; -; mRNA.
DR EMBL; AY160767; AAN78134.1; -; mRNA.
DR EMBL; AY160768; AAN78135.1; -; mRNA.
DR EMBL; AY160769; AAN78136.1; -; mRNA.
DR EMBL; AY279104; AAP40648.1; -; mRNA.
DR EMBL; AY279105; AAP40649.1; -; mRNA.
DR EMBL; AY279106; AAP40650.1; -; mRNA.
DR EMBL; AY279107; AAP40651.1; -; mRNA.
DR EMBL; AY279218; AAP33435.1; -; mRNA.
DR EMBL; AY279219; AAP33436.1; -; mRNA.
DR EMBL; AY279220; AAP33437.1; -; mRNA.
DR EMBL; AY513740; AAS15568.1; -; mRNA.
DR EMBL; DQ672577; ABG76001.1; -; Genomic_DNA.
DR AlphaFoldDB; Q5F0I3; -.
DR SMR; Q5F0I3; -.
DR PRIDE; Q5F0I3; -.
DR GO; GO:0030598; F:rRNA N-glycosylase activity; IEA:UniProtKB-EC.
DR GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR GO; GO:0006952; P:defense response; IEA:UniProtKB-KW.
DR GO; GO:0017148; P:negative regulation of translation; IEA:UniProtKB-KW.
DR Gene3D; 3.40.420.10; -; 1.
DR Gene3D; 4.10.470.10; -; 1.
DR InterPro; IPR036041; Ribosome-inact_prot_sf.
DR InterPro; IPR017989; Ribosome_inactivat_1/2.
DR InterPro; IPR001574; Ribosome_inactivat_prot.
DR InterPro; IPR017988; Ribosome_inactivat_prot_CS.
DR InterPro; IPR016138; Ribosome_inactivat_prot_sub1.
DR InterPro; IPR016139; Ribosome_inactivat_prot_sub2.
DR PANTHER; PTHR33453; PTHR33453; 1.
DR Pfam; PF00161; RIP; 1.
DR PRINTS; PR00396; SHIGARICIN.
DR SUPFAM; SSF56371; SSF56371; 1.
DR PROSITE; PS00275; SHIGA_RICIN; 1.
PE 1: Evidence at protein level;
KW Direct protein sequencing; Glycoprotein; Hydrolase; Plant defense;
KW Protein synthesis inhibitor; Signal; Toxin.
FT SIGNAL 1..23
FT /evidence="ECO:0000269|Ref.2"
FT CHAIN 24..300
FT /note="Ribosome-inactivating protein gynostemmin"
FT /id="PRO_0000271232"
FT ACT_SITE 184
FT /evidence="ECO:0000250|UniProtKB:P16094"
FT CARBOHYD 26
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 57
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 117
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 129
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 253
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 284
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CONFLICT 33
FT /note="D -> G (in Ref. 1; AAP40648/AAP40650)"
FT /evidence="ECO:0000305"
FT CONFLICT 38
FT /note="N -> K (in Ref. 1; AAP33436/AAP33435/AAP40648/
FT AAP40649/AAP40650)"
FT /evidence="ECO:0000305"
FT CONFLICT 40
FT /note="F -> R (in Ref. 1; AAN78135)"
FT /evidence="ECO:0000305"
FT CONFLICT 59
FT /note="T -> A (in Ref. 1; AAP40648)"
FT /evidence="ECO:0000305"
FT CONFLICT 66..94
FT /note="Missing (in Ref. 1; AAP40648/AAP40650)"
FT /evidence="ECO:0000305"
FT CONFLICT 76
FT /note="N -> S (in Ref. 1; AAP40649)"
FT /evidence="ECO:0000305"
FT CONFLICT 82..83
FT /note="GD -> NA (in Ref. 1; AAL82822/AAN08104/AAP33437/
FT AAP40649/AAP40651)"
FT /evidence="ECO:0000305"
FT CONFLICT 86
FT /note="T -> A (in Ref. 1; AAN78135)"
FT /evidence="ECO:0000305"
FT CONFLICT 105
FT /note="S -> P (in Ref. 1; AAP40649)"
FT /evidence="ECO:0000305"
FT CONFLICT 113
FT /note="D -> E (in Ref. 3; ABG76001)"
FT /evidence="ECO:0000305"
FT CONFLICT 118
FT /note="K -> Q (in Ref. 1; AAL82822/AAN08104/AAP33437/
FT AAP40651)"
FT /evidence="ECO:0000305"
FT CONFLICT 140
FT /note="E -> K (in Ref. 3; ABG76001)"
FT /evidence="ECO:0000305"
FT CONFLICT 142
FT /note="R -> G (in Ref. 1; AAL82822/AAN08104/AAP33437/
FT AAP40651/AAS15568)"
FT /evidence="ECO:0000305"
FT CONFLICT 145
FT /note="G -> R (in Ref. 1; AAL82822/AAN08104/AAP33437/
FT AAP40651/AAS15568)"
FT /evidence="ECO:0000305"
FT CONFLICT 157
FT /note="D -> N (in Ref. 1; AAL82822/AAN08104/AAP33437/
FT AAP40651/AAS15568)"
FT /evidence="ECO:0000305"
FT CONFLICT 164
FT /note="F -> L (in Ref. 1; AAN78135)"
FT /evidence="ECO:0000305"
FT CONFLICT 165
FT /note="R -> H (in Ref. 1; AAL82822/AAN08104/AAP33437/
FT AAP40651/AAS15568 and 3; ABG76001)"
FT /evidence="ECO:0000305"
FT CONFLICT 177
FT /note="V -> M (in Ref. 1; AAN78134)"
FT /evidence="ECO:0000305"
FT CONFLICT 192
FT /note="E -> G (in Ref. 1; AAP40649)"
FT /evidence="ECO:0000305"
FT CONFLICT 194
FT /note="K -> Q (in Ref. 1; AAL82822/AAN08104/AAP33437/
FT AAP40651/AAS15568)"
FT /evidence="ECO:0000305"
FT CONFLICT 202..203
FT /note="Missing (in Ref. 1; AAL82822/AAN08104/AAP33437/
FT AAP40649/AAP40651/AAS15568)"
FT /evidence="ECO:0000305"
FT CONFLICT 207
FT /note="P -> T (in Ref. 1; AAN08103)"
FT /evidence="ECO:0000305"
FT CONFLICT 209
FT /note="Q -> R (in Ref. 1; AAL82822/AAN08104/AAN78135/
FT AAP33437/AAP40649/AAP40651/AAS15568)"
FT /evidence="ECO:0000305"
FT CONFLICT 212
FT /note="I -> L (in Ref. 1; AAL82822/AAN08104/AAP33437/
FT AAP40649/AAP40651/AAS15568)"
FT /evidence="ECO:0000305"
FT CONFLICT 226..227
FT /note="QK -> LF (in Ref. 1; AAL82822)"
FT /evidence="ECO:0000305"
FT CONFLICT 230
FT /note="D -> G (in Ref. 1; AAL82822/AAN08104/AAP40649/
FT AAP40651/AAS15568)"
FT /evidence="ECO:0000305"
FT CONFLICT 238
FT /note="L -> P (in Ref. 1; AAL82822/AAN08104/AAP40649/
FT AAP40651/AAS15568 and 3; ABG76001)"
FT /evidence="ECO:0000305"
FT CONFLICT 245..248
FT /note="SGKP -> KNGS (in Ref. 1; AAL82822/AAN08104/AAP40649/
FT AAP40651/AAS15568)"
FT /evidence="ECO:0000305"
FT CONFLICT 268
FT /note="Y -> S (in Ref. 1; AAL82822/AAN08104/AAP40649/
FT AAP40651/AAS15568)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 300 AA; 33505 MW; A4860B0168BDD438 CRC64;
MRVARFCTVV AILLYFGFHI AECDINFSLA GADGQTYNTF IAKLRQELSI GTQKVANITV
LKHHVSSNTQ RFLSINLFNY NGDKITLGID VFDVYVVGFL TGTNSYIFKE APDLAYNKSL
LFPGSVRENL SYTGGYDDLE RRGAGREDIP LGLLPLDTAI TNLFRRDSTS FRRSFIVIIQ
MVSEAARFKI IEAKIAKNLY GENTFKPDQA IISLENNWGA LSKQIQKAQD RGGVFPNLVT
LTTSSGKPLI IRNDSDPLVQ NGIALLKYMS ERSVEYSITD DTVNESNGAF LEIMEIHDLM
