ID   RIP1_SAPOF              Reviewed;          40 AA.
AC   P98185;
DT   08-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-2000, sequence version 1.
DT   29-SEP-2021, entry version 63.
DE   RecName: Full=Ribosome-inactivating protein saporin-1;
DE            Short=SAP-1;
DE            EC=3.2.2.22;
DE   AltName: Full=SO-4;
DE   AltName: Full=rRNA N-glycosidase;
DE   Flags: Fragment;
GN   Name=SAP1;
OS   Saponaria officinalis (Common soapwort) (Lychnis saponaria).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   Caryophyllales; Caryophyllaceae; Caryophylleae; Saponaria.
OX   NCBI_TaxID=3572;
RN   [1]
RP   PROTEIN SEQUENCE.
RC   TISSUE=Leaf;
RX   PubMed=2753596; DOI=10.1111/j.1399-3011.1989.tb01280.x;
RA   Montecucchi P.-C., Lazzarini A.M., Barbieri L., Stirpe F., Soria M.,
RA   Lappi D.;
RT   "N-terminal sequence of some ribosome-inactivating proteins.";
RL   Int. J. Pept. Protein Res. 33:263-267(1989).
CC   -!- FUNCTION: Ribosome-inactivating protein of type 1, inhibits protein
CC       synthesis in animal cells. {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Endohydrolysis of the N-glycosidic bond at one specific
CC         adenosine on the 28S rRNA.; EC=3.2.2.22;
CC   -!- SIMILARITY: Belongs to the ribosome-inactivating protein family. Type 1
CC       RIP subfamily. {ECO:0000305}.
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DR   PIR; S16331; S16331.
DR   Allergome; 2805; Sap o RIP.
DR   GO; GO:0030598; F:rRNA N-glycosylase activity; IEA:UniProtKB-EC.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   GO; GO:0006952; P:defense response; IEA:UniProtKB-KW.
DR   GO; GO:0017148; P:negative regulation of translation; IEA:UniProtKB-KW.
DR   InterPro; IPR036041; Ribosome-inact_prot_sf.
DR   SUPFAM; SSF56371; SSF56371; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Hydrolase; Plant defense;
KW   Protein synthesis inhibitor; Toxin.
FT   CHAIN           1..>40
FT                   /note="Ribosome-inactivating protein saporin-1"
FT                   /id="PRO_0000221411"
FT   NON_TER         40
SQ   SEQUENCE   40 AA;  4625 MW;  F4615857A9871BF2 CRC64;
     VIIYELNLQG TTKAQYSTIL KQLRDDIKDP NLXYGXXDYS