ID   RIP2_DIACA              Reviewed;          60 AA.
AC   P24477;
DT   01-MAR-1992, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-1992, sequence version 1.
DT   29-SEP-2021, entry version 71.
DE   RecName: Full=Ribosome-inactivating protein dianthin-32 {ECO:0000305};
DE            Short=RIP DAP-32 {ECO:0000303|PubMed:1936243};
DE            EC=3.2.2.22 {ECO:0000250|UniProtKB:P84853};
DE   AltName: Full=Antiviral protein DAP-32 {ECO:0000303|PubMed:1936243};
DE   AltName: Full=rRNA N-glycosidase {ECO:0000305};
DE   Flags: Fragment;
OS   Dianthus caryophyllus (Carnation) (Clove pink).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   Caryophyllales; Caryophyllaceae; Caryophylleae; Dianthus.
OX   NCBI_TaxID=3570;
RN   [1]
RP   PROTEIN SEQUENCE, FUNCTION, AND MISCELLANEOUS.
RC   TISSUE=Leaf {ECO:0000303|PubMed:1936243};
RX   PubMed=1936243; DOI=10.1016/0014-5793(91)81122-o;
RA   Lee-Huang S., Kung H.-F., Huang P.L., Huang P.L., Li B.-Q., Huang P.,
RA   Huang H.I., Chen H.-C.;
RT   "A new class of anti-HIV agents: GAP31, DAPs 30 and 32.";
RL   FEBS Lett. 291:139-144(1991).
CC   -!- FUNCTION: Single-chain ribosome-inactivating protein.
CC       {ECO:0000269|PubMed:1936243}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Endohydrolysis of the N-glycosidic bond at one specific
CC         adenosine on the 28S rRNA.; EC=3.2.2.22;
CC         Evidence={ECO:0000250|UniProtKB:P84853};
CC   -!- MISCELLANEOUS: Possesses high antiviral potency and low toxicity to
CC       normal cells in culture and to intact animals. Capable of inhibiting
CC       HIV-1 infection and replication. {ECO:0000269|PubMed:1936243}.
CC   -!- SIMILARITY: Belongs to the ribosome-inactivating protein family. Type 1
CC       RIP subfamily. {ECO:0000305}.
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DR   PIR; S17686; S17686.
DR   GO; GO:0030598; F:rRNA N-glycosylase activity; IEA:UniProtKB-EC.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   GO; GO:0006952; P:defense response; IEA:UniProtKB-KW.
DR   GO; GO:0017148; P:negative regulation of translation; IEA:UniProtKB-KW.
DR   GO; GO:0050688; P:regulation of defense response to virus; IEA:UniProtKB-KW.
DR   Gene3D; 3.40.420.10; -; 1.
DR   InterPro; IPR036041; Ribosome-inact_prot_sf.
DR   InterPro; IPR016138; Ribosome_inactivat_prot_sub1.
DR   SUPFAM; SSF56371; SSF56371; 1.
PE   1: Evidence at protein level;
KW   Antiviral protein; Direct protein sequencing; Hydrolase; Pharmaceutical;
KW   Plant defense; Protein synthesis inhibitor; Toxin.
FT   CHAIN           1..>60
FT                   /note="Ribosome-inactivating protein dianthin-32"
FT                   /id="PRO_0000221419"
FT   NON_TER         60
SQ   SEQUENCE   60 AA;  6602 MW;  0F8EF1A1395CAD90 CRC64;
     AVKTITLNLV SPSANRYATF LTEIRDNVRX RSLDYSHSGI DVIGAPSSRD SXLNINFQSP