ID   RIP2_MOMBA              Reviewed;         286 AA.
AC   P29339;
DT   01-DEC-1992, integrated into UniProtKB/Swiss-Prot.
DT   01-DEC-1992, sequence version 1.
DT   25-MAY-2022, entry version 79.
DE   RecName: Full=Ribosome-inactivating protein momordin II;
DE            EC=3.2.2.22;
DE   AltName: Full=rRNA N-glycosidase;
DE   Flags: Precursor;
OS   Momordica balsamina (Bitter gourd) (Balsam apple).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; fabids; Cucurbitales; Cucurbitaceae; Momordiceae; Momordica.
OX   NCBI_TaxID=3672;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   TISSUE=Seed;
RX   PubMed=1408771; DOI=10.1093/nar/20.17.4662;
RA   Ortigao M., Better M.;
RT   "Momordin II, a ribosome inactivating protein from Momordica balsamina, is
RT   homologous to other plant proteins.";
RL   Nucleic Acids Res. 20:4662-4662(1992).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Endohydrolysis of the N-glycosidic bond at one specific
CC         adenosine on the 28S rRNA.; EC=3.2.2.22;
CC   -!- SIMILARITY: Belongs to the ribosome-inactivating protein family. Type 1
CC       RIP subfamily. {ECO:0000305}.
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DR   EMBL; Z12175; CAA78166.1; -; Genomic_DNA.
DR   PIR; S25560; S25560.
DR   AlphaFoldDB; P29339; -.
DR   BMRB; P29339; -.
DR   SMR; P29339; -.
DR   GO; GO:0030598; F:rRNA N-glycosylase activity; IEA:UniProtKB-EC.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   GO; GO:0006952; P:defense response; IEA:UniProtKB-KW.
DR   GO; GO:0017148; P:negative regulation of translation; IEA:UniProtKB-KW.
DR   Gene3D; 3.40.420.10; -; 1.
DR   Gene3D; 4.10.470.10; -; 1.
DR   InterPro; IPR036041; Ribosome-inact_prot_sf.
DR   InterPro; IPR017989; Ribosome_inactivat_1/2.
DR   InterPro; IPR001574; Ribosome_inactivat_prot.
DR   InterPro; IPR017988; Ribosome_inactivat_prot_CS.
DR   InterPro; IPR016138; Ribosome_inactivat_prot_sub1.
DR   InterPro; IPR016139; Ribosome_inactivat_prot_sub2.
DR   PANTHER; PTHR33453; PTHR33453; 1.
DR   Pfam; PF00161; RIP; 1.
DR   PRINTS; PR00396; SHIGARICIN.
DR   SUPFAM; SSF56371; SSF56371; 1.
DR   PROSITE; PS00275; SHIGA_RICIN; 1.
PE   3: Inferred from homology;
KW   Hydrolase; Plant defense; Protein synthesis inhibitor; Signal; Toxin.
FT   SIGNAL          1..23
FT   CHAIN           24..286
FT                   /note="Ribosome-inactivating protein momordin II"
FT                   /id="PRO_0000030772"
FT   ACT_SITE        181
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   286 AA;  32032 MW;  3B89FF1AE6B25986 CRC64;
     MVKCLLLSFL IIAIFIGVPT AKGDVNFDLS TATAKTYTKF IEDFRATLPF SHKVYDIPLL
     YSTISDSRRF ILLDLTSYAY ETISVAIDVT NVYVVAYRTR DVSYFFKESP PEAYNILFKG
     TRKITLPYTG NYENLQTAAH KIRENIDLGL PALSSAITTL FYYNAQSAPS ALLVLIQTTA
     EAARFKYIER HVAKYVATNF KPNLAIISLE NQWSALSKQI FLAQNQGGKF RNPVDLIKPT
     GERFQVTNVD SDVVKGNIKL LLNSRASTAD ENFITTMTLL GESVVN