ID   RIP2_SAPOF              Reviewed;         292 AA.
AC   P27559; Q41390; Q9SAP5;
DT   01-AUG-1992, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1992, sequence version 1.
DT   25-MAY-2022, entry version 98.
DE   RecName: Full=Ribosome-inactivating protein saporin-2;
DE            Short=SAP-2;
DE            Short=SO-2;
DE            EC=3.2.2.22;
DE   AltName: Full=rRNA N-glycosidase;
DE   Flags: Precursor;
GN   Name=SAP2;
OS   Saponaria officinalis (Common soapwort) (Lychnis saponaria).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   Caryophyllales; Caryophyllaceae; Caryophylleae; Saponaria.
OX   NCBI_TaxID=3572;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=1719367; DOI=10.1007/bf00267470;
RA   Fordham-Skelton A.P., Taylor P.E., Hartley M.R., Croy R.R.D.;
RT   "Characterisation of saporin genes: in vitro expression and ribosome
RT   inactivation.";
RL   Mol. Gen. Genet. 229:460-466(1991).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 25-284.
RC   TISSUE=Leaf;
RX   PubMed=8454624; DOI=10.1016/s0021-9258(18)53284-1;
RA   Barthelemy I., Martineau D., Ong M., Matsunami R., Ling N., Benatti L.,
RA   Cavallaro U., Soria M., Lappi D.A.;
RT   "The expression of saporin, a ribosome-inactivating protein from the plant
RT   Saponaria officinalis, in Escherichia coli.";
RL   J. Biol. Chem. 268:6541-6548(1993).
RN   [3]
RP   PROTEIN SEQUENCE OF 25-54.
RX   PubMed=8218413; DOI=10.1016/0167-4781(93)90034-b;
RA   Ferreras J.M., Barbieri L., Girbes T., Battelli M.G., Rojo M.A.,
RA   Arias F.J., Rocher M.A., Soriano F., Mendez E., Stirpe F.;
RT   "Distribution and properties of major ribosome-inactivating proteins (28 S
RT   rRNA N-glycosidases) of the plant Saponaria officinalis L.
RT   (Caryophyllaceae).";
RL   Biochim. Biophys. Acta 1216:31-42(1993).
CC   -!- FUNCTION: Ribosome-inactivating protein of type 1, inhibits protein
CC       synthesis in animal cells. Useful as immunotoxin for pharmacological
CC       applications.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Endohydrolysis of the N-glycosidic bond at one specific
CC         adenosine on the 28S rRNA.; EC=3.2.2.22;
CC   -!- SIMILARITY: Belongs to the ribosome-inactivating protein family. Type 1
CC       RIP subfamily. {ECO:0000305}.
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DR   EMBL; X59255; CAA41948.1; -; Genomic_DNA.
DR   EMBL; X69132; CAA48886.1; -; Genomic_DNA.
DR   EMBL; X69133; CAA48887.1; -; Genomic_DNA.
DR   PIR; S16487; S16487.
DR   PIR; S17933; RLQHG2.
DR   PIR; S38527; S38527.
DR   AlphaFoldDB; P27559; -.
DR   SMR; P27559; -.
DR   Allergome; 2805; Sap o RIP.
DR   GO; GO:0030598; F:rRNA N-glycosylase activity; IEA:UniProtKB-EC.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   GO; GO:0006952; P:defense response; IEA:UniProtKB-KW.
DR   GO; GO:0017148; P:negative regulation of translation; IEA:UniProtKB-KW.
DR   Gene3D; 3.40.420.10; -; 1.
DR   Gene3D; 4.10.470.10; -; 1.
DR   InterPro; IPR036041; Ribosome-inact_prot_sf.
DR   InterPro; IPR017989; Ribosome_inactivat_1/2.
DR   InterPro; IPR001574; Ribosome_inactivat_prot.
DR   InterPro; IPR017988; Ribosome_inactivat_prot_CS.
DR   InterPro; IPR016138; Ribosome_inactivat_prot_sub1.
DR   InterPro; IPR016139; Ribosome_inactivat_prot_sub2.
DR   PANTHER; PTHR33453; PTHR33453; 1.
DR   Pfam; PF00161; RIP; 1.
DR   PRINTS; PR00396; SHIGARICIN.
DR   SUPFAM; SSF56371; SSF56371; 1.
DR   PROSITE; PS00275; SHIGA_RICIN; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Hydrolase; Plant defense;
KW   Protein synthesis inhibitor; Signal; Toxin.
FT   SIGNAL          1..24
FT                   /evidence="ECO:0000269|PubMed:8218413"
FT   CHAIN           25..292
FT                   /note="Ribosome-inactivating protein saporin-2"
FT                   /id="PRO_0000030774"
FT   ACT_SITE        200
FT                   /evidence="ECO:0000250"
FT   VARIANT         72
FT                   /note="D -> E"
SQ   SEQUENCE   292 AA;  32811 MW;  FA143C0E1BE88976 CRC64;
     MKIYVVATIA WILLQFSAWT TTDAVTSITL DLVNPTAGQY SSFVDKIRNN VKDPNLKYGG
     TDIAVIGPPS KDKFLRINFQ SSRGTVSLGL KRDNLYVVAY LAMDNTNVNR AYYFKSEITS
     AELTALFPEA TTANQKALEY TEDYQSIEKN AQITQGDKSR KELGLGIDLL LTFMEAVNKK
     ARVVKNEARF LLIAIQMTAE VARFRYIQNL VTKNFPNKFD SDNKVIQFEV SWRKISTAIY
     GDAKNGVFNK DYDFGFGKVR QVKDLQMGLL MYLGKPKSSN EANSTAYATT VL