ID   RIP2_TRIKI              Reviewed;          45 AA.
AC   P23029;
DT   01-NOV-1991, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1991, sequence version 1.
DT   29-SEP-2021, entry version 70.
DE   RecName: Full=Ribosome-inactivating protein TAP-29;
DE            EC=3.2.2.22;
DE   AltName: Full=rRNA N-glycosidase;
DE   Flags: Fragment;
OS   Trichosanthes kirilowii (Chinese snake gourd) (Chinese cucumber).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; fabids; Cucurbitales; Cucurbitaceae; Sicyoeae; Trichosanthes.
OX   NCBI_TaxID=3677;
RN   [1]
RP   PROTEIN SEQUENCE.
RC   TISSUE=Tuberous root;
RX   PubMed=1713684; DOI=10.1073/pnas.88.15.6570;
RA   Lee-Huang S., Huang P.L., Kung H.-F., Li B.-Q., Huang P.L., Huang P.,
RA   Huang H.I., Chen H.-C.;
RT   "TAP 29: an anti-human immunodeficiency virus protein from Trichosanthes
RT   kirilowii that is nontoxic to intact cells.";
RL   Proc. Natl. Acad. Sci. U.S.A. 88:6570-6574(1991).
CC   -!- FUNCTION: Capable of inhibiting HIV-1 infection and replication. It
CC       inactivates eukaryotic 60S ribosomal subunits.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Endohydrolysis of the N-glycosidic bond at one specific
CC         adenosine on the 28S rRNA.; EC=3.2.2.22;
CC   -!- SIMILARITY: Belongs to the ribosome-inactivating protein family. Type 1
CC       RIP subfamily. {ECO:0000305}.
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DR   PIR; A39598; A39598.
DR   GO; GO:0030598; F:rRNA N-glycosylase activity; IEA:UniProtKB-EC.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   GO; GO:0006952; P:defense response; IEA:UniProtKB-KW.
DR   GO; GO:0017148; P:negative regulation of translation; IEA:UniProtKB-KW.
DR   InterPro; IPR036041; Ribosome-inact_prot_sf.
DR   SUPFAM; SSF56371; SSF56371; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Hydrolase; Plant defense;
KW   Protein synthesis inhibitor; Toxin.
FT   CHAIN           1..>45
FT                   /note="Ribosome-inactivating protein TAP-29"
FT                   /id="PRO_0000221409"
FT   NON_TER         45
SQ   SEQUENCE   45 AA;  5041 MW;  EE1178150C1CF7B7 CRC64;
     DVSFRLSGAT SKKKVYFISN LRKALPNEKK LYDIPLVRSS XSGSK