ID   RIP4_SAPOF              Reviewed;         157 AA.
AC   P27561;
DT   01-AUG-1992, integrated into UniProtKB/Swiss-Prot.
DT   01-APR-1993, sequence version 2.
DT   25-MAY-2022, entry version 84.
DE   RecName: Full=Ribosome-inactivating protein saporin-4;
DE            Short=SAP-4;
DE            EC=3.2.2.22;
DE   AltName: Full=SO-3;
DE   AltName: Full=rRNA N-glycosidase;
DE   Flags: Precursor; Fragment;
GN   Name=SAP4;
OS   Saponaria officinalis (Common soapwort) (Lychnis saponaria).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   Caryophyllales; Caryophyllaceae; Caryophylleae; Saponaria.
OX   NCBI_TaxID=3572;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=1719367; DOI=10.1007/bf00267470;
RA   Fordham-Skelton A.P., Taylor P.E., Hartley M.R., Croy R.R.D.;
RT   "Characterisation of saporin genes: in vitro expression and ribosome
RT   inactivation.";
RL   Mol. Gen. Genet. 229:460-466(1991).
CC   -!- FUNCTION: Ribosome-inactivating protein of type 1, inhibits protein
CC       synthesis in animal cells. Useful as immunotoxin for pharmacological
CC       applications.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Endohydrolysis of the N-glycosidic bond at one specific
CC         adenosine on the 28S rRNA.; EC=3.2.2.22;
CC   -!- SIMILARITY: Belongs to the ribosome-inactivating protein family. Type 1
CC       RIP subfamily. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=CAA41950.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR   EMBL; X59257; CAA41950.1; ALT_SEQ; Genomic_DNA.
DR   PIR; S16485; S16485.
DR   PIR; S16487; S16487.
DR   PIR; S17934; S17934.
DR   PIR; S38527; S38527.
DR   AlphaFoldDB; P27561; -.
DR   SMR; P27561; -.
DR   Allergome; 2805; Sap o RIP.
DR   GO; GO:0030598; F:rRNA N-glycosylase activity; IEA:UniProtKB-EC.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   GO; GO:0006952; P:defense response; IEA:UniProtKB-KW.
DR   GO; GO:0017148; P:negative regulation of translation; IEA:UniProtKB-KW.
DR   Gene3D; 3.40.420.10; -; 1.
DR   InterPro; IPR036041; Ribosome-inact_prot_sf.
DR   InterPro; IPR017989; Ribosome_inactivat_1/2.
DR   InterPro; IPR001574; Ribosome_inactivat_prot.
DR   InterPro; IPR016138; Ribosome_inactivat_prot_sub1.
DR   Pfam; PF00161; RIP; 1.
DR   PRINTS; PR00396; SHIGARICIN.
DR   SUPFAM; SSF56371; SSF56371; 1.
PE   3: Inferred from homology;
KW   Hydrolase; Plant defense; Protein synthesis inhibitor; Signal; Toxin.
FT   SIGNAL          1..24
FT   CHAIN           25..>157
FT                   /note="Ribosome-inactivating protein saporin-4"
FT                   /id="PRO_0000030775"
FT   NON_TER         157
SQ   SEQUENCE   157 AA;  17520 MW;  22DB3FFFDE18288C CRC64;
     MKIYVVATIA WILLQFSAWT TTDAVTSITL DLVNPTAGQY SSFVDKIRNN VKDPNLKYGG
     TDIAVIGPPS KGKFLRINFQ SSRGTVSLGL KRDNLYVVAY LAMDNTNVNR AYYFRSEITS
     AELTALFPEA TTANQKALEY TEDYQSIEKN AQITQED