ID   RIPL_LUFAE              Reviewed;          14 AA.
AC   P84073;
DT   16-AUG-2004, integrated into UniProtKB/Swiss-Prot.
DT   19-JUL-2004, sequence version 1.
DT   25-MAY-2022, entry version 35.
DE   RecName: Full=Ribosome-inactivating protein luffacylin;
DE            EC=3.2.2.22;
DE   AltName: Full=rRNA N-glycosidase;
DE   Flags: Fragment;
OS   Luffa aegyptiaca (Sponge gourd) (Luffa cylindrica).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; fabids; Cucurbitales; Cucurbitaceae; Sicyoeae; Luffa.
OX   NCBI_TaxID=3670;
RN   [1] {ECO:0000305}
RP   PROTEIN SEQUENCE, AND FUNCTION.
RC   TISSUE=Seed {ECO:0000269|PubMed:12126727};
RX   PubMed=12126727; DOI=10.1016/s0196-9781(02)00045-1;
RA   Parkash A., Ng T.B., Tso W.W.;
RT   "Isolation and characterization of luffacylin, a ribosome inactivating
RT   peptide with anti-fungal activity from sponge gourd (Luffa cylindrica)
RT   seeds.";
RL   Peptides 23:1019-1024(2002).
CC   -!- FUNCTION: Has antifungal activity against F.oxysporum and
CC       M.arachidicola. Inhibits cell-free translation in rabbit reticulocyte
CC       lysate system. Has some N-glycosidase activity.
CC       {ECO:0000269|PubMed:12126727}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Endohydrolysis of the N-glycosidic bond at one specific
CC         adenosine on the 28S rRNA.; EC=3.2.2.22;
CC         Evidence={ECO:0000269|PubMed:12126727};
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DR   GO; GO:0030598; F:rRNA N-glycosylase activity; IEA:UniProtKB-EC.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   GO; GO:0050832; P:defense response to fungus; IEA:UniProtKB-KW.
DR   GO; GO:0031640; P:killing of cells of another organism; IEA:UniProtKB-KW.
DR   GO; GO:0008152; P:metabolic process; IEA:UniProtKB-KW.
DR   GO; GO:0017148; P:negative regulation of translation; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   Antimicrobial; Direct protein sequencing; Fungicide; Glycosidase;
KW   Hydrolase; Protein synthesis inhibitor; Toxin.
FT   CHAIN           1..>14
FT                   /note="Ribosome-inactivating protein luffacylin"
FT                   /id="PRO_0000221425"
FT   NON_TER         14
FT                   /evidence="ECO:0000303|PubMed:12126727"
SQ   SEQUENCE   14 AA;  1646 MW;  87CB5B5507D44B01 CRC64;
     PRGSPRTEYE AARR