ID   RIP_ATV                 Reviewed;         145 AA.
AC   Q3V4R7;
DT   24-NOV-2009, integrated into UniProtKB/Swiss-Prot.
DT   11-OCT-2005, sequence version 1.
DT   25-MAY-2022, entry version 31.
DE   RecName: Full=RNAP inhibitory protein {ECO:0000305|PubMed:27882920};
DE            Short=RIP;
OS   Acidianus two-tailed virus (ATV).
OC   Viruses; Bicaudaviridae; Bicaudavirus.
OX   NCBI_TaxID=315953;
OH   NCBI_TaxID=269667; Acidianus convivator.
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=16121167; DOI=10.1038/4361101a;
RA   Haring M., Vestergaard G., Rachel R., Chen L., Garrett R.A.,
RA   Prangishvili D.;
RT   "Virology: independent virus development outside a host.";
RL   Nature 436:1101-1102(2005).
RN   [2]
RP   FUNCTION, AND INTERACTION WITH HOST RNAP SUBUNIT RPOA1.
RX   PubMed=27882920; DOI=10.1038/ncomms13595;
RA   Sheppard C., Blombach F., Belsom A., Schulz S., Daviter T., Smollett K.,
RA   Mahieu E., Erdmann S., Tinnefeld P., Garrett R., Grohmann D.,
RA   Rappsilber J., Werner F.;
RT   "Repression of RNA polymerase by the archaeo-viral regulator ORF145/RIP.";
RL   Nat. Commun. 7:13595-13595(2016).
CC   -!- FUNCTION: Plays a role in the inhibition of global transcription by
CC       interacting with the RNA polymerase clamp, locking it in a fixed
CC       position and inhibiting the formation and/or stability of the
CC       preinitiation complex (PIC). {ECO:0000269|PubMed:27882920}.
CC   -!- SUBUNIT: Interacts with host RNA polymerase subunit rpoA1.
CC       {ECO:0000269|PubMed:27882920}.
CC   -!- SUBCELLULAR LOCATION: Virion.
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DR   EMBL; AJ888457; CAI59897.1; -; Genomic_DNA.
DR   RefSeq; YP_319873.1; NC_007409.1.
DR   PDB; 7OQ4; EM; 3.27 A; Z=1-145.
DR   PDBsum; 7OQ4; -.
DR   SMR; Q3V4R7; -.
DR   GeneID; 4484253; -.
DR   KEGG; vg:4484253; -.
DR   Proteomes; UP000002150; Genome.
DR   GO; GO:0039653; P:suppression by virus of host transcription; IDA:UniProtKB.
PE   1: Evidence at protein level;
KW   3D-structure; Eukaryotic host gene expression shutoff by virus;
KW   Eukaryotic host transcription shutoff by virus;
KW   Host gene expression shutoff by virus; Host-virus interaction;
KW   Inhibition of eukaryotic host transcription initiation by virus;
KW   Reference proteome; Virion.
FT   CHAIN           1..145
FT                   /note="RNAP inhibitory protein"
FT                   /id="PRO_0000389051"
FT   HELIX           11..22
FT                   /evidence="ECO:0007829|PDB:7OQ4"
FT   HELIX           28..45
FT                   /evidence="ECO:0007829|PDB:7OQ4"
FT   HELIX           47..63
FT                   /evidence="ECO:0007829|PDB:7OQ4"
FT   STRAND          64..66
FT                   /evidence="ECO:0007829|PDB:7OQ4"
FT   STRAND          68..71
FT                   /evidence="ECO:0007829|PDB:7OQ4"
FT   HELIX           78..87
FT                   /evidence="ECO:0007829|PDB:7OQ4"
FT   HELIX           89..98
FT                   /evidence="ECO:0007829|PDB:7OQ4"
FT   HELIX           102..108
FT                   /evidence="ECO:0007829|PDB:7OQ4"
FT   STRAND          117..119
FT                   /evidence="ECO:0007829|PDB:7OQ4"
SQ   SEQUENCE   145 AA;  16834 MW;  EBA748991D239D6A CRC64;
     MKNMLHPQKY ETHVLDDLME FYEGVIGYPE IDLRLAGEEA WLKGVNPELA EAVKKIIKTI
     RRYLEGSPYD GSEKPIPRYI IAEIFSQIAP EVQLLVNALD TEGKYGFLKH IKKLNLNSLA
     MLSKNYNEND KLWKELENEG YVYLE