ID   RIP_CUCMO               Reviewed;         244 AA.
AC   P84531; B7SK17;
DT   24-MAY-2005, integrated into UniProtKB/Swiss-Prot.
DT   15-DEC-2009, sequence version 2.
DT   25-MAY-2022, entry version 43.
DE   RecName: Full=Ribosome-inactivating protein cucurmosin {ECO:0000303|PubMed:18652900};
DE            EC=3.2.2.22;
DE   AltName: Full=rRNA N-glycosidase {ECO:0000303|PubMed:18652900};
OS   Cucurbita moschata (Winter crookneck squash) (Cucurbita pepo var.
OS   moschata).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; fabids; Cucurbitales; Cucurbitaceae; Cucurbiteae; Cucurbita.
OX   NCBI_TaxID=3662;
RN   [1] {ECO:0000305, ECO:0000312|EMBL:ABY47624.1}
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-236, PROTEIN SEQUENCE OF 1-27, X-RAY
RP   CRYSTALLOGRAPHY (1.0 ANGSTROMS), FUNCTION, CATALYTIC ACTIVITY,
RP   GLYCOSYLATION AT ASN-225, AND ACTIVE SITES.
RC   TISSUE=Mesocarp {ECO:0000269|PubMed:18652900};
RX   PubMed=18652900; DOI=10.1016/j.jsb.2008.06.011;
RA   Hou X., Meehan E.J., Xie J., Huang M., Chen M., Chen L.;
RT   "Atomic resolution structure of cucurmosin, a novel type 1 ribosome-
RT   inactivating protein from the sarcocarp of Cucurbita moschata.";
RL   J. Struct. Biol. 164:81-87(2008).
CC   -!- FUNCTION: Has cytotoxic activity towards cancer cells, but not normal
CC       cells. Inhibits the growth of the human leukemia cell line K562, the
CC       murine melanoma cell line B16 and the lung adenocarcinoma cell line
CC       A549 with IC(50) values of 88.1 nM, 63.4 nM and 359.3 nM respectively.
CC       {ECO:0000269|PubMed:18652900}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Endohydrolysis of the N-glycosidic bond at one specific
CC         adenosine on the 28S rRNA.; EC=3.2.2.22;
CC         Evidence={ECO:0000269|PubMed:18652900};
CC   -!- PTM: The N-linked glycan consists of GlcNAc2Man3Xyl.
CC       {ECO:0000269|PubMed:18652900}.
CC   -!- SIMILARITY: Belongs to the ribosome-inactivating protein family. Type 1
CC       RIP subfamily. {ECO:0000255}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; EU309692; ABY47624.1; -; Genomic_DNA.
DR   PDB; 3BWH; X-ray; 1.00 A; A=1-244.
DR   PDBsum; 3BWH; -.
DR   AlphaFoldDB; P84531; -.
DR   SMR; P84531; -.
DR   iPTMnet; P84531; -.
DR   EvolutionaryTrace; P84531; -.
DR   GO; GO:0030598; F:rRNA N-glycosylase activity; IEA:UniProtKB-EC.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   GO; GO:0006952; P:defense response; IEA:UniProtKB-KW.
DR   GO; GO:0017148; P:negative regulation of translation; IEA:UniProtKB-KW.
DR   Gene3D; 3.40.420.10; -; 1.
DR   Gene3D; 4.10.470.10; -; 1.
DR   InterPro; IPR036041; Ribosome-inact_prot_sf.
DR   InterPro; IPR017989; Ribosome_inactivat_1/2.
DR   InterPro; IPR001574; Ribosome_inactivat_prot.
DR   InterPro; IPR017988; Ribosome_inactivat_prot_CS.
DR   InterPro; IPR016138; Ribosome_inactivat_prot_sub1.
DR   InterPro; IPR016139; Ribosome_inactivat_prot_sub2.
DR   PANTHER; PTHR33453; PTHR33453; 1.
DR   Pfam; PF00161; RIP; 1.
DR   PRINTS; PR00396; SHIGARICIN.
DR   SUPFAM; SSF56371; SSF56371; 1.
DR   PROSITE; PS00275; SHIGA_RICIN; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Direct protein sequencing; Glycoprotein; Hydrolase;
KW   Plant defense; Protein synthesis inhibitor; Toxin.
FT   CHAIN           1..244
FT                   /note="Ribosome-inactivating protein cucurmosin"
FT                   /id="PRO_0000221398"
FT   ACT_SITE        70
FT                   /evidence="ECO:0000269|PubMed:18652900"
FT   ACT_SITE        109
FT                   /evidence="ECO:0000269|PubMed:18652900"
FT   ACT_SITE        158
FT                   /evidence="ECO:0000269|PubMed:18652900"
FT   ACT_SITE        161
FT                   /evidence="ECO:0000269|PubMed:18652900"
FT   CARBOHYD        189
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        225
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000269|PubMed:18652900"
SQ   SEQUENCE   244 AA;  27008 MW;  EC055E4EA8AFB38C CRC64;
     NVRFDLSSAT SSSYKTFIKN LREALPKDGK VYDIPVLLST VMDSRRFILI DLVNYDGQSI
     TAAIDVLNVY IVAYSTGTVS YFFQQVPAQA PKLLFKGTQQ RTLPYTGNYE NLQTAAKKLR
     ENIELGLPAL DSAITTLFHY NAEAAASALL VLIQTTSEAA RFRYIELQIA NNVGTKFKPS
     QTIISLENNW SALSKQIQIA KNKNGQFETP VILIDPQGNR VQITNVTSNV VTQNIQLLLN
     IGAT