ID   RIP_FLAVE               Reviewed;          25 AA.
AC   P83324;
DT   10-OCT-2002, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2002, sequence version 1.
DT   02-JUN-2021, entry version 36.
DE   RecName: Full=Ribosome-inactivating protein velutin;
DE            EC=3.2.2.22;
DE   AltName: Full=rRNA N-glycosidase;
DE   Flags: Fragment;
OS   Flammulina velutipes (Agaricus velutipes).
OC   Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes;
OC   Agaricomycetidae; Agaricales; Physalacriaceae; Flammulina.
OX   NCBI_TaxID=38945;
RN   [1]
RP   PROTEIN SEQUENCE, AND FUNCTION.
RX   PubMed=11324720; DOI=10.1016/s0024-3205(01)01023-2;
RA   Wang H., Ng T.B.;
RT   "Isolation and characterization of velutin, a novel low-molecular-weight
RT   ribosome-inactivating protein from winter mushroom (Flammulina velutipes)
RT   fruiting bodies.";
RL   Life Sci. 68:2151-2158(2001).
CC   -!- FUNCTION: Inhibits protein synthesis but does not possess ribonuclease
CC       activity. Also inhibits HIV-1 reverse transcriptase, beta-glucosidase
CC       and beta-glucuronidase. {ECO:0000269|PubMed:11324720}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Endohydrolysis of the N-glycosidic bond at one specific
CC         adenosine on the 28S rRNA.; EC=3.2.2.22;
CC   -!- SIMILARITY: Belongs to the ribosome-inactivating protein family.
CC       {ECO:0000305}.
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DR   GO; GO:0030598; F:rRNA N-glycosylase activity; IDA:UniProtKB.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   GO; GO:0017148; P:negative regulation of translation; IDA:UniProtKB.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Hydrolase; Protein synthesis inhibitor; Toxin.
FT   CHAIN           1..>25
FT                   /note="Ribosome-inactivating protein velutin"
FT                   /id="PRO_0000221420"
FT   REGION          1..25
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   NON_TER         25
SQ   SEQUENCE   25 AA;  2898 MW;  EB73FCF9AA0993DB CRC64;
     XHPDLFXXRP DNTASPKFED PRLNP