ID   RITA1_XENLA             Reviewed;         239 AA.
AC   P0CJ62;
DT   08-FEB-2011, integrated into UniProtKB/Swiss-Prot.
DT   08-FEB-2011, sequence version 1.
DT   25-MAY-2022, entry version 25.
DE   RecName: Full=RBPJ-interacting and tubulin-associated protein 1;
DE   AltName: Full=RBPJ-interacting and tubulin-associated protein;
GN   Name=rita1; Synonyms=rita;
OS   Xenopus laevis (African clawed frog).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Xenopus.
OX   NCBI_TaxID=8355;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RG   NIH - Xenopus Gene Collection (XGC) project;
RL   Submitted (MAY-2004) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   FUNCTION, SUBCELLULAR LOCATION, MUTAGENESIS OF LEU-12; LEU-14 AND ILE-16,
RP   AND INTERACTION WITH RBPJ.
RX   PubMed=21102556; DOI=10.1038/emboj.2010.289;
RA   Wacker S.A., Alvarado C., von Wichert G., Knippschild U., Wiedenmann J.,
RA   Clauss K., Nienhaus G.U., Hameister H., Baumann B., Borggrefe T.,
RA   Knochel W., Oswald F.;
RT   "RITA, a novel modulator of Notch signalling, acts via nuclear export of
RT   RBP-J.";
RL   EMBO J. 30:43-56(2011).
CC   -!- FUNCTION: Tubulin-binding protein that acts as a negative regulator of
CC       Notch signaling pathway. Shuttles between the cytoplasm and the nucleus
CC       and mediates the nuclear export of rbpj/rbpsuh, thereby preventing the
CC       interaction between rbpj/rbpsuh and NICD product of Notch proteins
CC       (Notch intracellular domain), leading to down-regulate Notch-mediated
CC       transcription. May play a role in neurogenesis.
CC       {ECO:0000269|PubMed:21102556}.
CC   -!- SUBUNIT: Interacts with rbpj/rbpsuh. {ECO:0000269|PubMed:21102556}.
CC   -!- INTERACTION:
CC       P0CJ62; Q06330: RBPJ; Xeno; NbExp=2; IntAct=EBI-8517225, EBI-632552;
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:21102556}. Nucleus
CC       {ECO:0000269|PubMed:21102556}. Note=Shuttles rapidly between the
CC       cytoplasm and the nucleus.
CC   -!- SIMILARITY: Belongs to the RITA family. {ECO:0000305}.
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DR   EMBL; BC070862; -; NOT_ANNOTATED_CDS; mRNA.
DR   AlphaFoldDB; P0CJ62; -.
DR   ELM; P0CJ62; -.
DR   IntAct; P0CJ62; 2.
DR   MINT; P0CJ62; -.
DR   Proteomes; UP000186698; Genome assembly.
DR   GO; GO:0005737; C:cytoplasm; IDA:UniProtKB.
DR   GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR   GO; GO:0015631; F:tubulin binding; ISS:UniProtKB.
DR   GO; GO:0045746; P:negative regulation of Notch signaling pathway; IMP:UniProtKB.
DR   GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; TAS:UniProtKB.
DR   GO; GO:0022008; P:neurogenesis; IMP:UniProtKB.
DR   GO; GO:0007219; P:Notch signaling pathway; IEA:UniProtKB-KW.
DR   GO; GO:0051168; P:nuclear export; ISS:UniProtKB.
DR   InterPro; IPR031418; RITA1.
DR   PANTHER; PTHR34917; PTHR34917; 1.
DR   Pfam; PF17066; RITA; 1.
PE   1: Evidence at protein level;
KW   Cytoplasm; Neurogenesis; Notch signaling pathway; Nucleus;
KW   Reference proteome.
FT   CHAIN           1..239
FT                   /note="RBPJ-interacting and tubulin-associated protein 1"
FT                   /id="PRO_0000404585"
FT   REGION          62..97
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          129..158
FT                   /note="Interaction with RBPJ/RBPSUH"
FT                   /evidence="ECO:0000250"
FT   REGION          149..239
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          158..239
FT                   /note="Interaction with tubulin"
FT                   /evidence="ECO:0000250"
FT   MOTIF           12..24
FT                   /note="Nuclear export signal"
FT                   /evidence="ECO:0000305"
FT   MOTIF           93..109
FT                   /note="Nuclear localization signal"
FT                   /evidence="ECO:0000250"
FT   COMPBIAS        194..223
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MUTAGEN         12
FT                   /note="L->A: Results in nuclear accumulation; when
FT                   associated with A-14 and A-16."
FT                   /evidence="ECO:0000269|PubMed:21102556"
FT   MUTAGEN         14
FT                   /note="L->A: Results in nuclear accumulation; when
FT                   associated with A-12 and A-16."
FT                   /evidence="ECO:0000269|PubMed:21102556"
FT   MUTAGEN         16
FT                   /note="I->A: Results in nuclear accumulation; when
FT                   associated with A-12 and A-14."
FT                   /evidence="ECO:0000269|PubMed:21102556"
SQ   SEQUENCE   239 AA;  26637 MW;  D3EA216FC9371EF9 CRC64;
     MPDNLYATNM SLDLSITGHS TALPQRKSRS TYRFKAANSY VDESLFGSSG VRVDQTLQWN
     TAPPSQTPLL WSPGEIKENK KTSSCRPKST PAGTPRKKIQ YRVKSRTPSY CDESLFGGKV
     EECTWDAPWV KKEDTVKIRP LLWSPSPRLV QQSSMQNAKQ GPLRAVHPPE TSDSPLGTHK
     GLGAFWKPPE SDSDYSPSPF SARQRQSTPG RETVRSASCS GRVTARRGSV KMQERPPWK