RIX7_YEAST
ID RIX7_YEAST Reviewed; 837 AA.
AC Q07844; D6VXX1;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1997, sequence version 1.
DT 03-AUG-2022, entry version 162.
DE RecName: Full=Ribosome biogenesis ATPase RIX7;
GN Name=RIX7; OrderedLocusNames=YLL034C;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169871;
RA Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W.,
RA Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A.,
RA Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K.,
RA Heuss-Neitzel D., Hilbert H., Hilger F., Kleine K., Koetter P., Louis E.J.,
RA Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S.,
RA Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D.,
RA Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M.,
RA Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P.,
RA Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M.,
RA Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K.,
RA Zollner A., Hani J., Hoheisel J.D.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome XII.";
RL Nature 387:87-90(1997).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [3]
RP FUNCTION, AND SUBCELLULAR LOCATION.
RX PubMed=11447111; DOI=10.1093/emboj/20.14.3695;
RA Gadal O., Strauss D., Braspenning J., Hoepfner D., Petfalski E.,
RA Philippsen P., Tollervey D., Hurt E.;
RT "A nuclear AAA-type ATPase (Rix7p) is required for biogenesis and nuclear
RT export of 60S ribosomal subunits.";
RL EMBO J. 20:3695-3704(2001).
RN [4]
RP IDENTIFICATION BY MASS SPECTROMETRY.
RX PubMed=14690591; DOI=10.1016/s1097-2765(03)00476-3;
RA Hazbun T.R., Malmstroem L., Anderson S., Graczyk B.J., Fox B., Riffle M.,
RA Sundin B.A., Aranda J.D., McDonald W.H., Chiu C.-H., Snydsman B.E.,
RA Bradley P., Muller E.G.D., Fields S., Baker D., Yates J.R. III, Davis T.N.;
RT "Assigning function to yeast proteins by integration of technologies.";
RL Mol. Cell 12:1353-1365(2003).
RN [5]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
RN [6]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC STRAIN=ADR376;
RX PubMed=17330950; DOI=10.1021/pr060559j;
RA Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J.,
RA Elias J.E., Gygi S.P.;
RT "Large-scale phosphorylation analysis of alpha-factor-arrested
RT Saccharomyces cerevisiae.";
RL J. Proteome Res. 6:1190-1197(2007).
RN [7]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-42, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=19779198; DOI=10.1126/science.1172867;
RA Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.;
RT "Global analysis of Cdk1 substrate phosphorylation sites provides insights
RT into evolution.";
RL Science 325:1682-1686(2009).
CC -!- FUNCTION: Involved in ribosome biogenesis. Seems to be required for
CC restructuring nucleoplasmic 60S pre-ribosomal particles to make them
CC competent for nuclear export. {ECO:0000269|PubMed:11447111}.
CC -!- SUBCELLULAR LOCATION: Nucleus, nucleolus {ECO:0000269|PubMed:11447111}.
CC -!- MISCELLANEOUS: Present with 4490 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
CC -!- SIMILARITY: Belongs to the AAA ATPase family. {ECO:0000305}.
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DR EMBL; Z73139; CAA97483.1; -; Genomic_DNA.
DR EMBL; BK006945; DAA09287.1; -; Genomic_DNA.
DR PIR; S64785; S64785.
DR RefSeq; NP_013066.1; NM_001181854.1.
DR AlphaFoldDB; Q07844; -.
DR SMR; Q07844; -.
DR BioGRID; 31219; 88.
DR DIP; DIP-6385N; -.
DR IntAct; Q07844; 58.
DR MINT; Q07844; -.
DR STRING; 4932.YLL034C; -.
DR iPTMnet; Q07844; -.
DR MaxQB; Q07844; -.
DR PaxDb; Q07844; -.
DR PRIDE; Q07844; -.
DR EnsemblFungi; YLL034C_mRNA; YLL034C; YLL034C.
DR GeneID; 850625; -.
DR KEGG; sce:YLL034C; -.
DR SGD; S000003957; RIX7.
DR VEuPathDB; FungiDB:YLL034C; -.
DR eggNOG; KOG0733; Eukaryota.
DR GeneTree; ENSGT00570000079239; -.
DR HOGENOM; CLU_000688_8_2_1; -.
DR InParanoid; Q07844; -.
DR OMA; RQVFMRA; -.
DR BioCyc; YEAST:G3O-32137-MON; -.
DR PRO; PR:Q07844; -.
DR Proteomes; UP000002311; Chromosome XII.
DR RNAct; Q07844; protein.
DR GO; GO:0005730; C:nucleolus; IDA:SGD.
DR GO; GO:0005634; C:nucleus; IDA:SGD.
DR GO; GO:0030687; C:preribosome, large subunit precursor; IDA:SGD.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016887; F:ATP hydrolysis activity; ISS:SGD.
DR GO; GO:1990275; F:preribosome binding; IBA:GO_Central.
DR GO; GO:0051973; P:positive regulation of telomerase activity; IBA:GO_Central.
DR GO; GO:0042273; P:ribosomal large subunit biogenesis; IMP:SGD.
DR GO; GO:0000055; P:ribosomal large subunit export from nucleus; IMP:SGD.
DR GO; GO:0042254; P:ribosome biogenesis; IBA:GO_Central.
DR Gene3D; 3.40.50.300; -; 2.
DR InterPro; IPR003593; AAA+_ATPase.
DR InterPro; IPR041569; AAA_lid_3.
DR InterPro; IPR003959; ATPase_AAA_core.
DR InterPro; IPR003960; ATPase_AAA_CS.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR015415; Vps4_C.
DR Pfam; PF00004; AAA; 2.
DR Pfam; PF17862; AAA_lid_3; 2.
DR Pfam; PF09336; Vps4_C; 1.
DR SMART; SM00382; AAA; 2.
DR SUPFAM; SSF52540; SSF52540; 2.
DR PROSITE; PS00674; AAA; 1.
PE 1: Evidence at protein level;
KW ATP-binding; Nucleotide-binding; Nucleus; Phosphoprotein;
KW Reference proteome; Repeat; Ribosome biogenesis.
FT CHAIN 1..837
FT /note="Ribosome biogenesis ATPase RIX7"
FT /id="PRO_0000084762"
FT REGION 36..56
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 149..207
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 443..468
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 149..164
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 165..180
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 246..253
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255"
FT BINDING 574..581
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255"
FT MOD_RES 42
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:19779198"
SQ SEQUENCE 837 AA; 93069 MW; 133528B9D0987103 CRC64;
MVKVKSKKNS LTSSLDNKIV DLIYRLLEEK TLDRKRSLRQ ESQGEEGENN EGEEDEDIFE
SMFFAKDLTA GEIFTFCLTK DLSLQRVKKV VLQKTIDRML KDVIESELEE FGSYPGYNNE
EEEKPSLEEE LAKKNMMIER DTNEMNKRIT STWSKSGSVS ESITETDDPK TEEVKKSKKR
SKEGTCKVKR QKIKEDRSPP NSSLKSLGGM DDVVAQLMEL IGLPILHPEI FLSTGVEPPR
GVLLHGPPGC GKTSIANALA GELQVPFISI SAPSVVSGMS GESEKKIRDL FDEARSLAPC
LVFFDEIDAI TPKRDGGAQR EMERRIVAQL LTSMDELTME KTNGKPVIII GATNRPDSLD
AALRRAGRFD REICLNVPNE VSRLHILKKM SDNLKIDGAI DFAKLAKLTP GFVGADLKAL
VTAAGTCAIK RIFQTYANIK STPTTATDSS EDNMEIDETA NGDESSLKNT ANMIDPLPLS
VVQQFIRNYP EPLSGEQLSL LSIKYEDFLK ALPTIQPTAK REGFATVPDV TWANVGALQR
VRLELNMAIV QPIKRPELYE KVGISAPGGV LLWGPPGCGK TLLAKAVANE SRANFISIKG
PELLNKYVGE SERSIRQVFT RARASVPCVI FFDELDALVP RRDTSLSESS SRVVNTLLTE
LDGLNDRRGI FVIGATNRPD MIDPAMLRPG RLDKSLFIEL PNTEEKLDII KTLTKSHGTP
LSSDVDFEEI IRNEKCNNFS GADLAALVRE SSVLALKRKF FQSEEIQSVL DNDLDKEFED
LSVGVSGEEI IVTMSDFRSA LRKIKPSVSD KDRLKYDRLN KKMGLTEEMK DAEEMKQ
