RK10_SPIOL
ID RK10_SPIOL Reviewed; 232 AA.
AC P82139; A0A0K9R3N5; P82181; P82182;
DT 05-SEP-2006, integrated into UniProtKB/Swiss-Prot.
DT 12-APR-2017, sequence version 2.
DT 25-MAY-2022, entry version 54.
DE RecName: Full=50S ribosomal protein L10, chloroplastic {ECO:0000303|PubMed:10874046};
DE AltName: Full=CL10;
DE AltName: Full=Chloroplastic large ribosomal subunit protein uL10c {ECO:0000303|PubMed:28007896};
DE Flags: Precursor;
GN Name=RPL10; ORFNames=SOVF_110360;
OS Spinacia oleracea (Spinach).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC Caryophyllales; Chenopodiaceae; Chenopodioideae; Anserineae; Spinacia.
OX NCBI_TaxID=3562;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Viroflay; TISSUE=Leaf;
RX PubMed=24352233; DOI=10.1038/nature12817;
RA Dohm J.C., Minoche A.E., Holtgraewe D., Capella-Gutierrez S.,
RA Zakrzewski F., Tafer H., Rupp O., Soerensen T.R., Stracke R., Reinhardt R.,
RA Goesmann A., Kraft T., Schulz B., Stadler P.F., Schmidt T., Gabaldon T.,
RA Lehrach H., Weisshaar B., Himmelbauer H.;
RT "The genome of the recently domesticated crop plant sugar beet (Beta
RT vulgaris).";
RL Nature 505:546-549(2014).
RN [2]
RP PROTEIN SEQUENCE OF 53-77, SUBUNIT, SUBCELLULAR LOCATION, AND MASS
RP SPECTROMETRY.
RC STRAIN=cv. Alwaro; TISSUE=Leaf;
RX PubMed=10874046; DOI=10.1074/jbc.m005012200;
RA Yamaguchi K., Subramanian A.R.;
RT "The plastid ribosomal proteins. Identification of all the proteins in the
RT 50S subunit of an organelle ribosome (chloroplast).";
RL J. Biol. Chem. 275:28466-28482(2000).
RN [3]
RP STRUCTURE BY ELECTRON MICROSCOPY (3.25 ANGSTROMS), SUBUNIT, AND SUBCELLULAR
RP LOCATION.
RX PubMed=28007896; DOI=10.15252/embj.201695959;
RA Bieri P., Leibundgut M., Saurer M., Boehringer D., Ban N.;
RT "The complete structure of the chloroplast 70S ribosome in complex with
RT translation factor pY.";
RL EMBO J. 36:475-486(2017).
CC -!- FUNCTION: Component of the chloroplast ribosome (chloro-ribosome), a
CC dedicated translation machinery responsible for the synthesis of
CC chloroplast genome-encoded proteins, including proteins of the
CC transcription and translation machinery and components of the
CC photosynthetic apparatus. {ECO:0000305|PubMed:10874046,
CC ECO:0000305|PubMed:28007896}.
CC -!- SUBUNIT: Component of the chloroplast large ribosomal subunit (LSU).
CC Mature 70S chloroplast ribosomes of higher plants consist of a small
CC (30S) and a large (50S) subunit. The 30S small subunit contains 1
CC molecule of ribosomal RNA (16S rRNA) and 24 different proteins. The 50S
CC large subunit contains 3 rRNA molecules (23S, 5S and 4.5S rRNA) and 33
CC different proteins. {ECO:0000269|PubMed:10874046,
CC ECO:0000269|PubMed:28007896}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast
CC {ECO:0000269|PubMed:10874046, ECO:0000269|PubMed:28007896}.
CC -!- MASS SPECTROMETRY: Mass=20305.8; Method=Electrospray; Note=L10 alpha
CC form.; Evidence={ECO:0000269|PubMed:10874046};
CC -!- MISCELLANEOUS: Three forms, which differ in pI and possibly also in C-
CC terminal length, are produced, L10 alpha, L10 beta and L10 gamma.
CC {ECO:0000269|PubMed:10874046}.
CC -!- SIMILARITY: Belongs to the universal ribosomal protein uL10 family.
CC {ECO:0000305}.
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DR EMBL; KQ151220; KNA14105.1; -; Genomic_DNA.
DR PDB; 5MMI; EM; 3.25 A; I=1-232.
DR PDB; 5MMM; EM; 3.40 A; I=1-232.
DR PDBsum; 5MMI; -.
DR PDBsum; 5MMM; -.
DR AlphaFoldDB; P82139; -.
DR SMR; P82139; -.
DR STRING; 3562.P82139; -.
DR OrthoDB; 1343354at2759; -.
DR Proteomes; UP000054095; Unassembled WGS sequence.
DR GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR GO; GO:0015934; C:large ribosomal subunit; IBA:GO_Central.
DR GO; GO:0019843; F:rRNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003735; F:structural constituent of ribosome; IBA:GO_Central.
DR GO; GO:0006412; P:translation; IBA:GO_Central.
DR CDD; cd05797; Ribosomal_L10; 1.
DR Gene3D; 3.30.70.1730; -; 1.
DR HAMAP; MF_00362; Ribosomal_L10; 1.
DR InterPro; IPR022973; Ribosomal_L10.
DR InterPro; IPR043141; Ribosomal_L10-like_sf.
DR InterPro; IPR001790; Ribosomal_L10P.
DR PANTHER; PTHR11560; PTHR11560; 1.
DR Pfam; PF00466; Ribosomal_L10; 1.
DR SUPFAM; SSF160369; SSF160369; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Chloroplast; Direct protein sequencing; Plastid;
KW Reference proteome; Ribonucleoprotein; Ribosomal protein; RNA-binding;
KW rRNA-binding; Transit peptide.
FT TRANSIT 1..52
FT /note="Chloroplast"
FT /evidence="ECO:0000269|PubMed:10874046"
FT CHAIN 53..232
FT /note="50S ribosomal protein L10, chloroplastic"
FT /id="PRO_0000249186"
FT HELIX 56..71
FT /evidence="ECO:0007829|PDB:5MMI"
FT STRAND 74..81
FT /evidence="ECO:0007829|PDB:5MMI"
FT HELIX 86..99
FT /evidence="ECO:0007829|PDB:5MMI"
FT STRAND 101..104
FT /evidence="ECO:0007829|PDB:5MMI"
FT HELIX 107..116
FT /evidence="ECO:0007829|PDB:5MMI"
FT HELIX 120..126
FT /evidence="ECO:0007829|PDB:5MMI"
FT STRAND 127..129
FT /evidence="ECO:0007829|PDB:5MMI"
FT STRAND 131..134
FT /evidence="ECO:0007829|PDB:5MMI"
FT STRAND 136..138
FT /evidence="ECO:0007829|PDB:5MMI"
FT HELIX 140..153
FT /evidence="ECO:0007829|PDB:5MMI"
FT TURN 155..158
FT /evidence="ECO:0007829|PDB:5MMI"
FT STRAND 159..166
FT /evidence="ECO:0007829|PDB:5MMI"
FT STRAND 169..171
FT /evidence="ECO:0007829|PDB:5MMI"
FT TURN 173..176
FT /evidence="ECO:0007829|PDB:5MMI"
FT HELIX 177..181
FT /evidence="ECO:0007829|PDB:5MMI"
FT HELIX 185..188
FT /evidence="ECO:0007829|PDB:5MMI"
SQ SEQUENCE 232 AA; 26091 MW; 08808E2EBAACFD18 CRC64;
MESTLFLSKP LPTTIKTTTH SLSSVYPNPF KPNNLTFPRT THKHPTTTTI TAAISRTKKE
ETVETVQKHL ENCYLLAAIN YKGFTVKQFQ DLRKALPENT TLIVAKNTLV EKAVQDTQWA
AIKPCMKGMN AWLFVHSEEI PIAIKPYRTF QKERKLEDND FTGACFEGKF YGPGEVKRLE
TMPSKAEIFA KLLGSLKSPG SALVGTLQAP ARDLVFLLKA YIKKLEDEQQ QG
