RK12_SPIOL
ID RK12_SPIOL Reviewed; 189 AA.
AC P02398; Q53WU1;
DT 21-JUL-1986, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1990, sequence version 2.
DT 03-AUG-2022, entry version 111.
DE RecName: Full=50S ribosomal protein L12, chloroplastic {ECO:0000303|PubMed:10874046};
DE AltName: Full=CL12;
DE AltName: Full=Chloroplastic large ribosomal subunit protein bL12c {ECO:0000303|PubMed:28007896};
DE Flags: Precursor;
GN Name=RPL12; ORFNames=SOVF_040080;
OS Spinacia oleracea (Spinach).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC Caryophyllales; Chenopodiaceae; Chenopodioideae; Anserineae; Spinacia.
OX NCBI_TaxID=3562;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=2568127; DOI=10.1021/bi00434a056;
RA Giese K., Subramanian A.R.;
RT "Chloroplast ribosomal protein L12 is encoded in the nucleus: construction
RT and identification of its cDNA clones and nucleotide sequence including the
RT transit peptide.";
RL Biochemistry 28:3525-3529(1989).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Leaf;
RA Li Y., Zhou D.X., Seyer P., Massenet O., Dorne A.M., Mache R.;
RT "Analysis of two full length cDNAs coding for chloroplast precursor
RT ribosomal proteins homologous to the E. coli L12 and L24 ribosomal proteins
RT respectively.";
RL Submitted (OCT-1988) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Viroflay; TISSUE=Leaf;
RX PubMed=24352233; DOI=10.1038/nature12817;
RA Dohm J.C., Minoche A.E., Holtgraewe D., Capella-Gutierrez S.,
RA Zakrzewski F., Tafer H., Rupp O., Soerensen T.R., Stracke R., Reinhardt R.,
RA Goesmann A., Kraft T., Schulz B., Stadler P.F., Schmidt T., Gabaldon T.,
RA Lehrach H., Weisshaar B., Himmelbauer H.;
RT "The genome of the recently domesticated crop plant sugar beet (Beta
RT vulgaris).";
RL Nature 505:546-549(2014).
RN [4]
RP PROTEIN SEQUENCE OF 57-189.
RC STRAIN=cv. Alwaro; TISSUE=Leaf;
RX PubMed=16593249; DOI=10.1073/pnas.79.22.6871;
RA Bartsch M., Kimura M., Subramanian A.R.;
RT "Purification, primary structure, and homology relationships of a
RT chloroplast ribosomal protein.";
RL Proc. Natl. Acad. Sci. U.S.A. 79:6871-6875(1982).
RN [5]
RP SEQUENCE REVISION TO 113-115.
RA Bartsch M., Kimura M., Subramanian A.R.;
RL Submitted (NOV-1999) to UniProtKB.
RN [6]
RP PROTEIN SEQUENCE OF 57-62, SUBUNIT, SUBCELLULAR LOCATION, AND MASS
RP SPECTROMETRY.
RC STRAIN=cv. Alwaro; TISSUE=Leaf;
RX PubMed=10874046; DOI=10.1074/jbc.m005012200;
RA Yamaguchi K., Subramanian A.R.;
RT "The plastid ribosomal proteins. Identification of all the proteins in the
RT 50S subunit of an organelle ribosome (chloroplast).";
RL J. Biol. Chem. 275:28466-28482(2000).
RN [7]
RP MODELING ON THE 70S RIBOSOME.
RX PubMed=18042701; DOI=10.1073/pnas.0709856104;
RA Sharma M.R., Wilson D.N., Datta P.P., Barat C., Schluenzen F., Fucini P.,
RA Agrawal R.K.;
RT "Cryo-EM study of the spinach chloroplast ribosome reveals the structural
RT and functional roles of plastid-specific ribosomal proteins.";
RL Proc. Natl. Acad. Sci. U.S.A. 104:19315-19320(2007).
RN [8]
RP NOMENCLATURE, SUBUNIT, AND SUBCELLULAR LOCATION.
RX PubMed=28007896; DOI=10.15252/embj.201695959;
RA Bieri P., Leibundgut M., Saurer M., Boehringer D., Ban N.;
RT "The complete structure of the chloroplast 70S ribosome in complex with
RT translation factor pY.";
RL EMBO J. 36:475-486(2017).
CC -!- FUNCTION: Component of the chloroplast ribosome (chloro-ribosome), a
CC dedicated translation machinery responsible for the synthesis of
CC chloroplast genome-encoded proteins, including proteins of the
CC transcription and translation machinery and components of the
CC photosynthetic apparatus. {ECO:0000305|PubMed:10874046,
CC ECO:0000305|PubMed:28007896}.
CC -!- SUBUNIT: Component of the chloroplast large ribosomal subunit (LSU).
CC Mature 70S chloroplast ribosomes of higher plants consist of a small
CC (30S) and a large (50S) subunit. The 30S small subunit contains 1
CC molecule of ribosomal RNA (16S rRNA) and 24 different proteins. The 50S
CC large subunit contains 3 rRNA molecules (23S, 5S and 4.5S rRNA) and 33
CC different proteins. {ECO:0000269|PubMed:10874046,
CC ECO:0000269|PubMed:28007896}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast
CC {ECO:0000269|PubMed:10874046, ECO:0000269|PubMed:28007896}.
CC -!- MASS SPECTROMETRY: Mass=13813.0; Method=Electrospray;
CC Evidence={ECO:0000269|PubMed:10874046};
CC -!- SIMILARITY: Belongs to the bacterial ribosomal protein bL12 family.
CC {ECO:0000305}.
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DR EMBL; J02849; AAA34031.1; -; mRNA.
DR EMBL; X13153; CAA31551.1; -; mRNA.
DR EMBL; KQ136668; KNA21798.1; -; Genomic_DNA.
DR PIR; A30115; R7SP12.
DR AlphaFoldDB; P02398; -.
DR SMR; P02398; -.
DR STRING; 3562.P02398; -.
DR PRIDE; P02398; -.
DR OrthoDB; 1626139at2759; -.
DR Proteomes; UP000054095; Unassembled WGS sequence.
DR GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR GO; GO:0005840; C:ribosome; IEA:UniProtKB-KW.
DR GO; GO:0003729; F:mRNA binding; IBA:GO_Central.
DR GO; GO:0003735; F:structural constituent of ribosome; IBA:GO_Central.
DR GO; GO:0006412; P:translation; IBA:GO_Central.
DR CDD; cd00387; Ribosomal_L7_L12; 1.
DR Gene3D; 1.20.5.710; -; 1.
DR Gene3D; 3.30.1390.10; -; 1.
DR HAMAP; MF_00368; Ribosomal_L7_L12; 1.
DR InterPro; IPR000206; Ribosomal_L7/12.
DR InterPro; IPR014719; Ribosomal_L7/12_C/ClpS-like.
DR InterPro; IPR013823; Ribosomal_L7/L12_C.
DR InterPro; IPR008932; Ribosomal_L7/L12_oligo.
DR InterPro; IPR036235; Ribosomal_L7/L12_oligo_N_sf.
DR PANTHER; PTHR45987; PTHR45987; 1.
DR Pfam; PF00542; Ribosomal_L12; 1.
DR Pfam; PF16320; Ribosomal_L12_N; 1.
DR SUPFAM; SSF48300; SSF48300; 1.
DR SUPFAM; SSF54736; SSF54736; 1.
DR TIGRFAMs; TIGR00855; L12; 1.
PE 1: Evidence at protein level;
KW Chloroplast; Direct protein sequencing; Plastid; Reference proteome;
KW Ribonucleoprotein; Ribosomal protein; RNA-binding; Transit peptide.
FT TRANSIT 1..56
FT /note="Chloroplast"
FT /evidence="ECO:0000269|PubMed:10874046,
FT ECO:0000269|PubMed:16593249"
FT CHAIN 57..189
FT /note="50S ribosomal protein L12, chloroplastic"
FT /id="PRO_0000030454"
FT REGION 1..30
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 165..189
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 165..181
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 189 AA; 19934 MW; E04A50CF9D47C5E7 CRC64;
MAATTTMATL NLPSLTSHPN SSTFPKHPQP LQFPFRTTTN PISLSSTRTT RLRPIAAVEA
PEKIEQLGTQ LSGLTLEEAR VLVDWLQDKL GVSAASFAPA AAVAAPGAPA DAAPAVEEKT
EFDVSIDEVP SNARISVIKA VRALTSLGLK EAKELIEGLP KKLKEGVSKD DAEDAKKQLE
DAGAKVSIV
