RK17_SPIOL
ID RK17_SPIOL Reviewed; 126 AA.
AC P82194; A0A0K9RLJ4;
DT 05-SEP-2006, integrated into UniProtKB/Swiss-Prot.
DT 12-APR-2017, sequence version 2.
DT 25-MAY-2022, entry version 55.
DE RecName: Full=50S ribosomal protein L17, chloroplastic {ECO:0000303|PubMed:10874046};
DE AltName: Full=CL17;
DE AltName: Full=Chloroplastic large ribosomal subunit protein bL17c {ECO:0000303|PubMed:28007896};
DE Flags: Precursor;
GN Name=RPL17; ORFNames=SOVF_059230;
OS Spinacia oleracea (Spinach).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC Caryophyllales; Chenopodiaceae; Chenopodioideae; Anserineae; Spinacia.
OX NCBI_TaxID=3562;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Viroflay; TISSUE=Leaf;
RX PubMed=24352233; DOI=10.1038/nature12817;
RA Dohm J.C., Minoche A.E., Holtgraewe D., Capella-Gutierrez S.,
RA Zakrzewski F., Tafer H., Rupp O., Soerensen T.R., Stracke R., Reinhardt R.,
RA Goesmann A., Kraft T., Schulz B., Stadler P.F., Schmidt T., Gabaldon T.,
RA Lehrach H., Weisshaar B., Himmelbauer H.;
RT "The genome of the recently domesticated crop plant sugar beet (Beta
RT vulgaris).";
RL Nature 505:546-549(2014).
RN [2]
RP PROTEIN SEQUENCE OF 11-32, SUBUNIT, SUBCELLULAR LOCATION, AND MASS
RP SPECTROMETRY.
RC STRAIN=cv. Alwaro; TISSUE=Leaf;
RX PubMed=10874046; DOI=10.1074/jbc.m005012200;
RA Yamaguchi K., Subramanian A.R.;
RT "The plastid ribosomal proteins. Identification of all the proteins in the
RT 50S subunit of an organelle ribosome (chloroplast).";
RL J. Biol. Chem. 275:28466-28482(2000).
RN [3]
RP STRUCTURE BY ELECTRON MICROSCOPY (9.4 ANGSTROMS).
RX PubMed=18042701; DOI=10.1073/pnas.0709856104;
RA Sharma M.R., Wilson D.N., Datta P.P., Barat C., Schluenzen F., Fucini P.,
RA Agrawal R.K.;
RT "Cryo-EM study of the spinach chloroplast ribosome reveals the structural
RT and functional roles of plastid-specific ribosomal proteins.";
RL Proc. Natl. Acad. Sci. U.S.A. 104:19315-19320(2007).
RN [4]
RP STRUCTURE BY ELECTRON MICROSCOPY (3.50 ANGSTROMS).
RX PubMed=27762343; DOI=10.1038/srep35793;
RA Ahmed T., Yin Z., Bhushan S.;
RT "Cryo-EM structure of the large subunit of the spinach chloroplast
RT ribosome.";
RL Sci. Rep. 6:35793-35793(2016).
RN [5]
RP STRUCTURE BY ELECTRON MICROSCOPY (3.25 ANGSTROMS), SUBUNIT, AND SUBCELLULAR
RP LOCATION.
RX PubMed=28007896; DOI=10.15252/embj.201695959;
RA Bieri P., Leibundgut M., Saurer M., Boehringer D., Ban N.;
RT "The complete structure of the chloroplast 70S ribosome in complex with
RT translation factor pY.";
RL EMBO J. 36:475-486(2017).
CC -!- FUNCTION: Component of the chloroplast ribosome (chloro-ribosome), a
CC dedicated translation machinery responsible for the synthesis of
CC chloroplast genome-encoded proteins, including proteins of the
CC transcription and translation machinery and components of the
CC photosynthetic apparatus. {ECO:0000305|PubMed:10874046,
CC ECO:0000305|PubMed:28007896}.
CC -!- SUBUNIT: Component of the chloroplast large ribosomal subunit (LSU).
CC Mature 70S chloroplast ribosomes of higher plants consist of a small
CC (30S) and a large (50S) subunit. The 30S small subunit contains 1
CC molecule of ribosomal RNA (16S rRNA) and 24 different proteins. The 50S
CC large subunit contains 3 rRNA molecules (23S, 5S and 4.5S rRNA) and 33
CC different proteins. {ECO:0000269|PubMed:10874046,
CC ECO:0000269|PubMed:28007896}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast
CC {ECO:0000269|PubMed:10874046, ECO:0000269|PubMed:28007896}.
CC -!- MASS SPECTROMETRY: Mass=13022.4; Method=Electrospray;
CC Evidence={ECO:0000269|PubMed:10874046};
CC -!- SIMILARITY: Belongs to the bacterial ribosomal protein bL17 family.
CC {ECO:0000255}.
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DR EMBL; KQ140075; KNA19692.1; -; Genomic_DNA.
DR PDB; 4V61; EM; 9.40 A; P=23-32.
DR PDB; 5H1S; EM; 3.50 A; P=11-126.
DR PDB; 5MLC; EM; 3.90 A; P=1-126.
DR PDB; 5MMI; EM; 3.25 A; O=1-126.
DR PDB; 5MMM; EM; 3.40 A; O=1-126.
DR PDB; 5X8P; EM; 3.40 A; O=11-126.
DR PDB; 5X8T; EM; 3.30 A; O=11-126.
DR PDB; 6ERI; EM; 3.00 A; AN=11-126.
DR PDBsum; 4V61; -.
DR PDBsum; 5H1S; -.
DR PDBsum; 5MLC; -.
DR PDBsum; 5MMI; -.
DR PDBsum; 5MMM; -.
DR PDBsum; 5X8P; -.
DR PDBsum; 5X8T; -.
DR PDBsum; 6ERI; -.
DR AlphaFoldDB; P82194; -.
DR SMR; P82194; -.
DR IntAct; P82194; 1.
DR STRING; 3562.P82194; -.
DR Proteomes; UP000054095; Unassembled WGS sequence.
DR GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR GO; GO:0022625; C:cytosolic large ribosomal subunit; IBA:GO_Central.
DR GO; GO:0015934; C:large ribosomal subunit; IBA:GO_Central.
DR GO; GO:0019843; F:rRNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003735; F:structural constituent of ribosome; IBA:GO_Central.
DR GO; GO:0006412; P:translation; IEA:InterPro.
DR Gene3D; 3.90.1030.10; -; 1.
DR HAMAP; MF_01368; Ribosomal_L17; 1.
DR InterPro; IPR000456; Ribosomal_L17.
DR InterPro; IPR036373; Ribosomal_L17_sf.
DR PANTHER; PTHR14413; PTHR14413; 1.
DR Pfam; PF01196; Ribosomal_L17; 1.
DR SUPFAM; SSF64263; SSF64263; 1.
DR TIGRFAMs; TIGR00059; L17; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Chloroplast; Direct protein sequencing; Plastid;
KW Reference proteome; Ribonucleoprotein; Ribosomal protein; RNA-binding;
KW rRNA-binding; Transit peptide.
FT TRANSIT 1..10
FT /note="Chloroplast"
FT /evidence="ECO:0000269|PubMed:10874046"
FT CHAIN 11..126
FT /note="50S ribosomal protein L17, chloroplastic"
FT /id="PRO_0000249230"
FT HELIX 24..41
FT /evidence="ECO:0007829|PDB:5MMI"
FT STRAND 42..47
FT /evidence="ECO:0007829|PDB:5MMI"
FT HELIX 48..67
FT /evidence="ECO:0007829|PDB:5MMI"
FT HELIX 70..77
FT /evidence="ECO:0007829|PDB:5MMI"
FT HELIX 83..96
FT /evidence="ECO:0007829|PDB:5MMI"
FT TURN 97..100
FT /evidence="ECO:0007829|PDB:5H1S"
FT STRAND 105..110
FT /evidence="ECO:0007829|PDB:5MMI"
FT TURN 114..116
FT /evidence="ECO:0007829|PDB:5MMI"
FT STRAND 120..125
FT /evidence="ECO:0007829|PDB:5MMI"
SQ SEQUENCE 126 AA; 14544 MW; 4E31F663C2706EEC CRC64;
MIDNGGRFFA MKHGRKIHRL SRPADQRRAL LRGLTTQLLK HGRIKTTRAK ASAMRKYVDK
MITLAKEGSL HKRRQALGFI YEKQIVHALF AEVPERYGDR NGGYTRIIRT LPRRGDNAPM
AYIELV
