RK1_SPIOL
ID RK1_SPIOL Reviewed; 352 AA.
AC Q9LE95; A0A0K9QF10; P82178;
DT 05-SEP-2006, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 25-MAY-2022, entry version 83.
DE RecName: Full=50S ribosomal protein L1, chloroplastic {ECO:0000303|PubMed:10874046};
DE AltName: Full=CL1;
DE AltName: Full=Chloroplastic large ribosomal subunit protein uL1c {ECO:0000303|PubMed:28007896};
DE Flags: Precursor;
GN Name=RPL1; ORFNames=SOVF_186350;
OS Spinacia oleracea (Spinach).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC Caryophyllales; Chenopodiaceae; Chenopodioideae; Anserineae; Spinacia.
OX NCBI_TaxID=3562;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=cv. Matador;
RA Kavousi M., Webster C., Wegloehner W., Gray J., Subramanian A.R.;
RL Submitted (DEC-1993) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Viroflay; TISSUE=Leaf;
RX PubMed=24352233; DOI=10.1038/nature12817;
RA Dohm J.C., Minoche A.E., Holtgraewe D., Capella-Gutierrez S.,
RA Zakrzewski F., Tafer H., Rupp O., Soerensen T.R., Stracke R., Reinhardt R.,
RA Goesmann A., Kraft T., Schulz B., Stadler P.F., Schmidt T., Gabaldon T.,
RA Lehrach H., Weisshaar B., Himmelbauer H.;
RT "The genome of the recently domesticated crop plant sugar beet (Beta
RT vulgaris).";
RL Nature 505:546-549(2014).
RN [3]
RP PROTEIN SEQUENCE OF 73-95; 110-116; 119-121; 152-158; 162-176; 189-193;
RP 203-207; 225-231; 238-260; 275-278; 305-312; 320-322 AND 338-349.
RA Yamaguchi K., von Knoblauch K., Subramanian A.R.;
RL Submitted (NOV-1999) to UniProtKB.
RN [4]
RP PROTEIN SEQUENCE OF 73-78, SUBUNIT, SUBCELLULAR LOCATION, AND MASS
RP SPECTROMETRY.
RC STRAIN=cv. Alwaro; TISSUE=Leaf;
RX PubMed=10874046; DOI=10.1074/jbc.m005012200;
RA Yamaguchi K., Subramanian A.R.;
RT "The plastid ribosomal proteins. Identification of all the proteins in the
RT 50S subunit of an organelle ribosome (chloroplast).";
RL J. Biol. Chem. 275:28466-28482(2000).
RN [5]
RP STRUCTURE BY ELECTRON MICROSCOPY (9.4 ANGSTROMS).
RX PubMed=18042701; DOI=10.1073/pnas.0709856104;
RA Sharma M.R., Wilson D.N., Datta P.P., Barat C., Schluenzen F., Fucini P.,
RA Agrawal R.K.;
RT "Cryo-EM study of the spinach chloroplast ribosome reveals the structural
RT and functional roles of plastid-specific ribosomal proteins.";
RL Proc. Natl. Acad. Sci. U.S.A. 104:19315-19320(2007).
RN [6]
RP NOMENCLATURE, SUBUNIT, AND SUBCELLULAR LOCATION.
RX PubMed=28007896; DOI=10.15252/embj.201695959;
RA Bieri P., Leibundgut M., Saurer M., Boehringer D., Ban N.;
RT "The complete structure of the chloroplast 70S ribosome in complex with
RT translation factor pY.";
RL EMBO J. 36:475-486(2017).
CC -!- FUNCTION: Component of the chloroplast ribosome (chloro-ribosome), a
CC dedicated translation machinery responsible for the synthesis of
CC chloroplast genome-encoded proteins, including proteins of the
CC transcription and translation machinery and components of the
CC photosynthetic apparatus. {ECO:0000305|PubMed:10874046,
CC ECO:0000305|PubMed:28007896}.
CC -!- SUBUNIT: Component of the chloroplast large ribosomal subunit (LSU).
CC Mature 70S chloroplast ribosomes of higher plants consist of a small
CC (30S) and a large (50S) subunit. The 30S small subunit contains 1
CC molecule of ribosomal RNA (16S rRNA) and 24 different proteins. The 50S
CC large subunit contains 3 rRNA molecules (23S, 5S and 4.5S rRNA) and 33
CC different proteins. {ECO:0000269|PubMed:10874046,
CC ECO:0000269|PubMed:28007896}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast
CC {ECO:0000269|PubMed:10874046, ECO:0000269|PubMed:28007896}.
CC -!- MASS SPECTROMETRY: Mass=30447.0; Method=Electrospray;
CC Evidence={ECO:0000269|PubMed:10874046};
CC -!- SIMILARITY: Belongs to the universal ribosomal protein uL1 family.
CC {ECO:0000255}.
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DR EMBL; X76932; CAA54255.1; -; mRNA.
DR EMBL; KQ187486; KNA05869.1; -; Genomic_DNA.
DR PIR; T51935; T51935.
DR PDB; 4V61; EM; 9.40 A; BD=1-352.
DR PDBsum; 4V61; -.
DR AlphaFoldDB; Q9LE95; -.
DR SMR; Q9LE95; -.
DR STRING; 3562.Q9LE95; -.
DR OrthoDB; 1432931at2759; -.
DR EvolutionaryTrace; Q9LE95; -.
DR Proteomes; UP000054095; Unassembled WGS sequence.
DR GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR GO; GO:0022625; C:cytosolic large ribosomal subunit; IBA:GO_Central.
DR GO; GO:0003723; F:RNA binding; IBA:GO_Central.
DR GO; GO:0019843; F:rRNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003735; F:structural constituent of ribosome; IEA:InterPro.
DR GO; GO:0000470; P:maturation of LSU-rRNA; IBA:GO_Central.
DR GO; GO:0006412; P:translation; IEA:InterPro.
DR CDD; cd00403; Ribosomal_L1; 1.
DR Gene3D; 3.40.50.790; -; 1.
DR HAMAP; MF_01318_B; Ribosomal_L1_B; 1.
DR InterPro; IPR005878; Ribosom_L1_bac-type.
DR InterPro; IPR023674; Ribosomal_L1-like.
DR InterPro; IPR028364; Ribosomal_L1/biogenesis.
DR InterPro; IPR016095; Ribosomal_L1_3-a/b-sand.
DR InterPro; IPR023673; Ribosomal_L1_CS.
DR Pfam; PF00687; Ribosomal_L1; 1.
DR SUPFAM; SSF56808; SSF56808; 1.
DR TIGRFAMs; TIGR01169; rplA_bact; 1.
DR PROSITE; PS01199; RIBOSOMAL_L1; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Chloroplast; Direct protein sequencing; Plastid;
KW Reference proteome; Ribonucleoprotein; Ribosomal protein; RNA-binding;
KW rRNA-binding; Transit peptide.
FT TRANSIT 1..72
FT /note="Chloroplast"
FT /evidence="ECO:0000269|PubMed:10874046, ECO:0000269|Ref.3"
FT CHAIN 73..352
FT /note="50S ribosomal protein L1, chloroplastic"
FT /id="PRO_0000249343"
FT REGION 82..113
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 295
FT /note="E -> K (in Ref. 2; KNA05869)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 352 AA; 38628 MW; 5179E698B071DA91 CRC64;
MATAAPPSLS LCYASSSFQY QQDPSFQTHF KPLLLNSSLC RLTLNQRERS CLKWVDFTSQ
KQSPKSVSFR VLAAVAAEAE VADMEEEEGE SGGVATLPSP TKPKKGKAAL PLKSDRTRSK
RFLEIQKLRE IKQEYDLKTA LSLMKQMSST KFVETAEAHF RLNIDPKYND QQLRATVSLP
KGTGKTVKIA VLAQGDKIDE AKAAGADIVG GEELIEQIKG GFMDFDKLIA TSDMMAKVAS
LGRILGPRGL MPTPKAGTVT PNVAQAVEEF KKGKVEFRVD KTGIVHIPFG KLNFEEEDLL
INLFATIKSV ETNKPTGAKG VYWKSAHISS SMGPSIRLNI REMLDYKPPS NA
