AAP7_ARATH
ID AAP7_ARATH Reviewed; 467 AA.
AC Q9FF99; Q2V350; Q941E6;
DT 03-NOV-2009, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 03-AUG-2022, entry version 111.
DE RecName: Full=Probable amino acid permease 7;
DE AltName: Full=Amino acid transporter AAP7;
GN Name=AAP7; OrderedLocusNames=At5g23810; ORFNames=MRO11.15;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RX PubMed=12244056; DOI=10.1074/jbc.m207730200;
RA Okumoto S., Schmidt R., Tegeder M., Fischer W.-N., Rentsch D.,
RA Frommer W.B., Koch W.;
RT "High affinity amino acid transporters specifically expressed in xylem
RT parenchyma and developing seeds of Arabidopsis.";
RL J. Biol. Chem. 277:45338-45346(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=9330910; DOI=10.1093/dnares/4.3.215;
RA Sato S., Kotani H., Nakamura Y., Kaneko T., Asamizu E., Fukami M.,
RA Miyajima N., Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. I. Sequence
RT features of the 1.6 Mb regions covered by twenty physically assigned P1
RT clones.";
RL DNA Res. 4:215-230(1997).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=cv. Columbia;
RX PubMed=19423640; DOI=10.1093/dnares/dsp009;
RA Iida K., Fukami-Kobayashi K., Toyoda A., Sakaki Y., Kobayashi M., Seki M.,
RA Shinozaki K.;
RT "Analysis of multiple occurrences of alternative splicing events in
RT Arabidopsis thaliana using novel sequenced full-length cDNAs.";
RL DNA Res. 16:155-164(2009).
CC -!- FUNCTION: Amino acid-proton symporter. Stereospecific transporter with
CC a broad specificity for neutral amino acids (By similarity).
CC {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q9FF99-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q9FF99-2; Sequence=VSP_038275, VSP_038276;
CC -!- SIMILARITY: Belongs to the amino acid/polyamine transporter 2 family.
CC Amino acid/auxin permease (AAAP) (TC 2.A.18.2) subfamily.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAK97680.1; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; AB005244; BAB10054.1; -; Genomic_DNA.
DR EMBL; CP002688; AED93215.1; -; Genomic_DNA.
DR EMBL; CP002688; AED93216.1; -; Genomic_DNA.
DR EMBL; AY052209; AAK97680.1; ALT_FRAME; mRNA.
DR EMBL; AY143854; AAN28793.1; -; mRNA.
DR EMBL; AK316999; BAH19694.1; -; mRNA.
DR RefSeq; NP_001031934.1; NM_001036857.1. [Q9FF99-2]
DR RefSeq; NP_197770.1; NM_122286.4. [Q9FF99-1]
DR AlphaFoldDB; Q9FF99; -.
DR BioGRID; 17721; 1.
DR IntAct; Q9FF99; 1.
DR STRING; 3702.AT5G23810.1; -.
DR TCDB; 2.A.18.2.10; the amino acid/auxin permease (aaap) family.
DR PaxDb; Q9FF99; -.
DR PRIDE; Q9FF99; -.
DR ProteomicsDB; 245100; -. [Q9FF99-1]
DR EnsemblPlants; AT5G23810.1; AT5G23810.1; AT5G23810. [Q9FF99-1]
DR EnsemblPlants; AT5G23810.2; AT5G23810.2; AT5G23810. [Q9FF99-2]
DR GeneID; 832446; -.
DR Gramene; AT5G23810.1; AT5G23810.1; AT5G23810. [Q9FF99-1]
DR Gramene; AT5G23810.2; AT5G23810.2; AT5G23810. [Q9FF99-2]
DR KEGG; ath:AT5G23810; -.
DR Araport; AT5G23810; -.
DR TAIR; locus:2172868; AT5G23810.
DR eggNOG; KOG1303; Eukaryota.
DR HOGENOM; CLU_031247_4_0_1; -.
DR InParanoid; Q9FF99; -.
DR OMA; IKRANCY; -.
DR OrthoDB; 570025at2759; -.
DR PhylomeDB; Q9FF99; -.
DR PRO; PR:Q9FF99; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q9FF99; baseline and differential.
DR Genevisible; Q9FF99; AT.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0016020; C:membrane; ISS:TAIR.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0015293; F:symporter activity; IEA:UniProtKB-KW.
DR GO; GO:0006865; P:amino acid transport; ISS:TAIR.
DR InterPro; IPR013057; AA_transpt_TM.
DR Pfam; PF01490; Aa_trans; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Amino-acid transport; Cell membrane; Membrane;
KW Reference proteome; Symport; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..467
FT /note="Probable amino acid permease 7"
FT /id="PRO_0000387505"
FT TOPO_DOM 1..29
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 30..50
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 51..58
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 59..79
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 80..111
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 112..132
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 133..163
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 164..184
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 185..205
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 206..231
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 232..252
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 253..274
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 275..295
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 296..312
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 313..333
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 334..383
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 384..404
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 405..425
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 426..443
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 444..464
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 465..467
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT VAR_SEQ 334..361
FT /note="YSQPIFAAAERSLTKKYPENKFIARFYG -> SQKPLAHLTNMLVDFYVFVH
FT ILENKLPF (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:19423640"
FT /id="VSP_038275"
FT VAR_SEQ 362..467
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:19423640"
FT /id="VSP_038276"
SQ SEQUENCE 467 AA; 51723 MW; 1BE41C85CE364406 CRC64;
MDIKEDDESR VITPTELQLH DSVTARTGTL WTAVAHIITG VIGAGVLSLA WATAELGWIA
GPAALIAFAG VTLLSAFLLS DCYRFPDPNN GPLRLNSYSQ AVKLYLGKKN EIVCGVVVYI
SLFGCGIAYT IVIATCSRAI MKSNCYHRNG HNATCSYGDN NNYFMVLFGL TQIFMSQIPN
FHNMVWLSLV AAIMSFTYSF IGIGLALGKI IENRKIEGSI RGIPAENRGE KVWIVFQALG
NIAFSYPFSI ILLEIQDTLR SPPAEKQTMK KASTVAVFIQ TFFFFCCGCF GYAAFGDSTP
GNLLTGFGFY EPFWLVDFAN ACIVLHLVGG YQVYSQPIFA AAERSLTKKY PENKFIARFY
GFKLPLLRGE TVRLNPMRMC LRTMYVLITT GVAVMFPYFN EVLGVVGALA FWPLAVYFPV
EMCILQKKIR SWTRPWLLLR GFSFVCLLVC LLSLVGSIYG LVGAKFG
