AAP8_ARATH
ID AAP8_ARATH Reviewed; 475 AA.
AC O80592;
DT 03-NOV-2009, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1998, sequence version 1.
DT 03-AUG-2022, entry version 110.
DE RecName: Full=Amino acid permease 8;
DE AltName: Full=Amino acid transporter AAP8;
GN Name=AAP8; OrderedLocusNames=At1g10010; ORFNames=T27I1.3;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, AND
RP BIOPHYSICOCHEMICAL PROPERTIES.
RX PubMed=12244056; DOI=10.1074/jbc.m207730200;
RA Okumoto S., Schmidt R., Tegeder M., Fischer W.-N., Rentsch D.,
RA Frommer W.B., Koch W.;
RT "High affinity amino acid transporters specifically expressed in xylem
RT parenchyma and developing seeds of Arabidopsis.";
RL J. Biol. Chem. 277:45338-45346(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=17476526; DOI=10.1007/s00425-007-0527-x;
RA Schmidt R., Stransky H., Koch W.;
RT "The amino acid permease AAP8 is important for early seed development in
RT Arabidopsis thaliana.";
RL Planta 226:805-813(2007).
CC -!- FUNCTION: Amino acid-proton symporter. Stereospecific transporter with
CC a broad specificity for glutamate, aspartate and neutral and acidic
CC amino acids. {ECO:0000269|PubMed:17476526}.
CC -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC Kinetic parameters:
CC KM=444 uM for aspartate {ECO:0000269|PubMed:12244056};
CC Vmax=1.7 nmol/min/mg enzyme toward aspartate
CC {ECO:0000269|PubMed:12244056};
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- TISSUE SPECIFICITY: Expressed in flower buds, siliques, developing
CC seeds and funiculi. {ECO:0000269|PubMed:12244056}.
CC -!- DEVELOPMENTAL STAGE: Found in young seeds 2 to 5 days after
CC fertilization, but not expressed in mature seeds.
CC {ECO:0000269|PubMed:12244056}.
CC -!- DISRUPTION PHENOTYPE: Reduced seed number and silique size correlated
CC with a specifically altered amino acid composition of young siliques.
CC {ECO:0000269|PubMed:17476526}.
CC -!- SIMILARITY: Belongs to the amino acid/polyamine transporter 2 family.
CC Amino acid/auxin permease (AAAP) (TC 2.A.18.2) subfamily.
CC {ECO:0000305}.
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DR EMBL; AC004122; AAC34329.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE28528.1; -; Genomic_DNA.
DR PIR; T00620; T00620.
DR RefSeq; NP_172472.1; NM_100875.1.
DR AlphaFoldDB; O80592; -.
DR SMR; O80592; -.
DR BioGRID; 22776; 9.
DR IntAct; O80592; 6.
DR STRING; 3702.AT1G10010.1; -.
DR TCDB; 2.A.18.2.5; the amino acid/auxin permease (aaap) family.
DR PaxDb; O80592; -.
DR PRIDE; O80592; -.
DR EnsemblPlants; AT1G10010.1; AT1G10010.1; AT1G10010.
DR GeneID; 837536; -.
DR Gramene; AT1G10010.1; AT1G10010.1; AT1G10010.
DR KEGG; ath:AT1G10010; -.
DR Araport; AT1G10010; -.
DR TAIR; locus:2201871; AT1G10010.
DR eggNOG; KOG1303; Eukaryota.
DR HOGENOM; CLU_031247_4_1_1; -.
DR InParanoid; O80592; -.
DR OMA; WLVIFAN; -.
DR PhylomeDB; O80592; -.
DR SABIO-RK; O80592; -.
DR PRO; PR:O80592; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; O80592; baseline and differential.
DR Genevisible; O80592; AT.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0016020; C:membrane; ISS:TAIR.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0015172; F:acidic amino acid transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0015171; F:amino acid transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0015175; F:neutral amino acid transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0015293; F:symporter activity; IEA:UniProtKB-KW.
DR GO; GO:0015800; P:acidic amino acid transport; IBA:GO_Central.
DR GO; GO:0003333; P:amino acid transmembrane transport; IBA:GO_Central.
DR GO; GO:0015804; P:neutral amino acid transport; IBA:GO_Central.
DR InterPro; IPR013057; AA_transpt_TM.
DR Pfam; PF01490; Aa_trans; 1.
PE 1: Evidence at protein level;
KW Amino-acid transport; Cell membrane; Membrane; Reference proteome; Symport;
KW Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..475
FT /note="Amino acid permease 8"
FT /id="PRO_0000387506"
FT TOPO_DOM 1..31
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 32..52
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 53..73
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 74..120
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 121..141
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 142..157
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 158..178
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 179..185
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 186..206
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 207..236
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 237..257
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 258..276
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 277..297
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 298..314
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 315..335
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 336..378
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 379..398
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 399..401
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 402..424
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 425..441
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 442..462
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 463..475
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT REGION 1..22
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 475 AA; 51815 MW; B6DEE571E2FE7F92 CRC64;
MDAYNNPSAV ESGDAAVKSV DDDGREKRTG TFWTASAHII TAVIGSGVLS LAWAIAQLGW
VAGTTVLVAF AIITYYTSTL LADCYRSPDS ITGTRNYNYM GVVRSYLGGK KVQLCGVAQY
VNLVGVTIGY TITASISLVA IGKSNCYHDK GHKAKCSVSN YPYMAAFGIV QIILSQLPNF
HKLSFLSIIA AVMSFSYASI GIGLAIATVA SGKIGKTELT GTVIGVDVTA SEKVWKLFQA
IGDIAFSYAF TTILIEIQDT LRSSPPENKV MKRASLVGVS TTTVFYILCG CIGYAAFGNQ
APGDFLTDFG FYEPYWLIDF ANACIALHLI GAYQVYAQPF FQFVEENCNK KWPQSNFINK
EYSSKVPLLG KCRVNLFRLV WRTCYVVLTT FVAMIFPFFN AILGLLGAFA FWPLTVYFPV
AMHIAQAKVK KYSRRWLALN LLVLVCLIVS ALAAVGSIIG LINSVKSYKP FKNLD
