RK35_SPIOL
ID RK35_SPIOL Reviewed; 159 AA.
AC P23326; A0A0K9RCY8;
DT 01-NOV-1991, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1991, sequence version 1.
DT 03-AUG-2022, entry version 95.
DE RecName: Full=50S ribosomal protein L35, chloroplastic {ECO:0000303|PubMed:10874046};
DE AltName: Full=CL35;
DE AltName: Full=Chloroplastic large ribosomal subunit protein bL35c {ECO:0000303|PubMed:28007896};
DE Flags: Precursor;
GN Name=RPL35; ORFNames=SOVF_080710;
OS Spinacia oleracea (Spinach).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC Caryophyllales; Chenopodiaceae; Chenopodioideae; Anserineae; Spinacia.
OX NCBI_TaxID=3562;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND PROTEIN SEQUENCE OF 87-135.
RC STRAIN=cv. Alwaro;
RX PubMed=2271612; DOI=10.1021/bi00493a032;
RA Smooker P.M., Choli T., Subramanian A.R.;
RT "Ribosomal protein L35: identification in spinach chloroplasts and
RT isolation of a cDNA clone encoding its cytoplasmic precursor.";
RL Biochemistry 29:9733-9736(1990).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=2398071; DOI=10.1016/s0021-9258(17)46277-6;
RA Smooker P.M., Kruft V., Subramanian A.R.;
RT "A ribosomal protein is encoded in the chloroplast DNA in a lower plant but
RT in the nucleus in angiosperms. Isolation of the spinach L21 protein and
RT cDNA clone with transit and an unusual repeat sequence.";
RL J. Biol. Chem. 265:16699-16703(1990).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Viroflay; TISSUE=Leaf;
RX PubMed=24352233; DOI=10.1038/nature12817;
RA Dohm J.C., Minoche A.E., Holtgraewe D., Capella-Gutierrez S.,
RA Zakrzewski F., Tafer H., Rupp O., Soerensen T.R., Stracke R., Reinhardt R.,
RA Goesmann A., Kraft T., Schulz B., Stadler P.F., Schmidt T., Gabaldon T.,
RA Lehrach H., Weisshaar B., Himmelbauer H.;
RT "The genome of the recently domesticated crop plant sugar beet (Beta
RT vulgaris).";
RL Nature 505:546-549(2014).
RN [4]
RP PROTEIN SEQUENCE OF 87-94, SUBUNIT, SUBCELLULAR LOCATION, AND MASS
RP SPECTROMETRY.
RC STRAIN=cv. Alwaro; TISSUE=Leaf;
RX PubMed=10874046; DOI=10.1074/jbc.m005012200;
RA Yamaguchi K., Subramanian A.R.;
RT "The plastid ribosomal proteins. Identification of all the proteins in the
RT 50S subunit of an organelle ribosome (chloroplast).";
RL J. Biol. Chem. 275:28466-28482(2000).
RN [5]
RP STRUCTURE BY ELECTRON MICROSCOPY (9.4 ANGSTROMS).
RX PubMed=18042701; DOI=10.1073/pnas.0709856104;
RA Sharma M.R., Wilson D.N., Datta P.P., Barat C., Schluenzen F., Fucini P.,
RA Agrawal R.K.;
RT "Cryo-EM study of the spinach chloroplast ribosome reveals the structural
RT and functional roles of plastid-specific ribosomal proteins.";
RL Proc. Natl. Acad. Sci. U.S.A. 104:19315-19320(2007).
RN [6]
RP STRUCTURE BY ELECTRON MICROSCOPY (3.50 ANGSTROMS).
RX PubMed=27762343; DOI=10.1038/srep35793;
RA Ahmed T., Yin Z., Bhushan S.;
RT "Cryo-EM structure of the large subunit of the spinach chloroplast
RT ribosome.";
RL Sci. Rep. 6:35793-35793(2016).
RN [7]
RP STRUCTURE BY ELECTRON MICROSCOPY (3.25 ANGSTROMS), SUBUNIT, AND SUBCELLULAR
RP LOCATION.
RX PubMed=28007896; DOI=10.15252/embj.201695959;
RA Bieri P., Leibundgut M., Saurer M., Boehringer D., Ban N.;
RT "The complete structure of the chloroplast 70S ribosome in complex with
RT translation factor pY.";
RL EMBO J. 36:475-486(2017).
CC -!- FUNCTION: Component of the chloroplast ribosome (chloro-ribosome), a
CC dedicated translation machinery responsible for the synthesis of
CC chloroplast genome-encoded proteins, including proteins of the
CC transcription and translation machinery and components of the
CC photosynthetic apparatus. {ECO:0000305|PubMed:10874046,
CC ECO:0000305|PubMed:28007896}.
CC -!- SUBUNIT: Component of the chloroplast large ribosomal subunit (LSU).
CC Mature 70S chloroplast ribosomes of higher plants consist of a small
CC (30S) and a large (50S) subunit. The 30S small subunit contains 1
CC molecule of ribosomal RNA (16S rRNA) and 24 different proteins. The 50S
CC large subunit contains 3 rRNA molecules (23S, 5S and 4.5S rRNA) and 33
CC different proteins. {ECO:0000269|PubMed:10874046,
CC ECO:0000269|PubMed:28007896}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast
CC {ECO:0000269|PubMed:10874046, ECO:0000269|PubMed:28007896}.
CC -!- MASS SPECTROMETRY: Mass=8449.9; Method=Electrospray;
CC Evidence={ECO:0000269|PubMed:10874046};
CC -!- SIMILARITY: Belongs to the bacterial ribosomal protein bL35 family.
CC {ECO:0000305}.
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DR EMBL; M60449; AAA34043.1; -; mRNA.
DR EMBL; KQ144065; KNA17358.1; -; Genomic_DNA.
DR PIR; A36107; A36107.
DR PDB; 4V61; EM; 9.40 A; B5=1-159.
DR PDB; 5H1S; EM; 3.50 A; e=87-159.
DR PDB; 5MLC; EM; 3.90 A; 5=1-159.
DR PDB; 5MMI; EM; 3.25 A; 4=1-159.
DR PDB; 5MMM; EM; 3.40 A; 4=1-159.
DR PDB; 5X8P; EM; 3.40 A; 4=87-159.
DR PDB; 5X8T; EM; 3.30 A; 4=87-159.
DR PDB; 6ERI; EM; 3.00 A; Ad=88-159.
DR PDBsum; 4V61; -.
DR PDBsum; 5H1S; -.
DR PDBsum; 5MLC; -.
DR PDBsum; 5MMI; -.
DR PDBsum; 5MMM; -.
DR PDBsum; 5X8P; -.
DR PDBsum; 5X8T; -.
DR PDBsum; 6ERI; -.
DR AlphaFoldDB; P23326; -.
DR SMR; P23326; -.
DR IntAct; P23326; 1.
DR STRING; 3562.P23326; -.
DR PRIDE; P23326; -.
DR EvolutionaryTrace; P23326; -.
DR Proteomes; UP000054095; Unassembled WGS sequence.
DR GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR GO; GO:0015934; C:large ribosomal subunit; IBA:GO_Central.
DR GO; GO:0003735; F:structural constituent of ribosome; IBA:GO_Central.
DR GO; GO:0006412; P:translation; IEA:InterPro.
DR Gene3D; 4.10.410.60; -; 1.
DR HAMAP; MF_00514; Ribosomal_L35; 1.
DR InterPro; IPR021137; Ribosomal_L35.
DR InterPro; IPR018265; Ribosomal_L35_CS.
DR InterPro; IPR001706; Ribosomal_L35_non-mt.
DR InterPro; IPR037229; Ribosomal_L35_sf.
DR PANTHER; PTHR33343; PTHR33343; 1.
DR Pfam; PF01632; Ribosomal_L35p; 1.
DR PRINTS; PR00064; RIBOSOMALL35.
DR SUPFAM; SSF143034; SSF143034; 1.
DR TIGRFAMs; TIGR00001; rpmI_bact; 1.
DR PROSITE; PS00936; RIBOSOMAL_L35; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Chloroplast; Direct protein sequencing; Plastid;
KW Reference proteome; Ribonucleoprotein; Ribosomal protein; Transit peptide.
FT TRANSIT 1..86
FT /note="Chloroplast"
FT /evidence="ECO:0000269|PubMed:10874046,
FT ECO:0000269|PubMed:2271612"
FT CHAIN 87..159
FT /note="50S ribosomal protein L35, chloroplastic"
FT /id="PRO_0000030524"
FT HELIX 96..99
FT /evidence="ECO:0007829|PDB:5MMI"
FT STRAND 102..104
FT /evidence="ECO:0007829|PDB:5X8T"
FT STRAND 106..108
FT /evidence="ECO:0007829|PDB:5X8T"
FT STRAND 110..112
FT /evidence="ECO:0007829|PDB:5MMI"
FT STRAND 122..124
FT /evidence="ECO:0007829|PDB:5X8T"
FT HELIX 126..131
FT /evidence="ECO:0007829|PDB:5MMI"
FT STRAND 134..137
FT /evidence="ECO:0007829|PDB:5X8T"
FT HELIX 140..142
FT /evidence="ECO:0007829|PDB:5MMI"
FT HELIX 143..149
FT /evidence="ECO:0007829|PDB:5MMI"
SQ SEQUENCE 159 AA; 17341 MW; C247FE36470007D1 CRC64;
MAMASATATL SFKTPSLSLS PPSTRCSAAQ GISLTHFNKQ LKSTLNLSSS SSISSSKVQP
IVLKNKRIST VDSSVSTSSP SFTVFAAKGY KMKTHKASAK RFRVTGKGKI VRRRAGKQHL
LAKKNTKRKN RLSKLIQVDR SDYDNVIGAL PYLKVNRKV
