RK4_ARATH
ID RK4_ARATH Reviewed; 282 AA.
AC O50061; Q8LF27; Q8VZS1; Q9LNV1;
DT 15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT 20-JUN-2002, sequence version 2.
DT 25-MAY-2022, entry version 139.
DE RecName: Full=50S ribosomal protein L4, chloroplastic;
DE AltName: Full=CL4;
DE AltName: Full=R-protein L4;
DE Flags: Precursor;
GN Name=RPL4; OrderedLocusNames=At1g07320; ORFNames=F22G5.34, F22G5_28;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=9461586; DOI=10.1074/jbc.273.7.3980;
RA Trifa Y., Privat I., Gagnon J., Baeza L., Lerbs-Mache S.;
RT "The nuclear RPL4 gene encodes a chloroplast protein that co-purifies with
RT the T7-like transcription complex as well as plastid ribosomes.";
RL J. Biol. Chem. 273:3980-3985(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: This protein binds directly and specifically to 23S rRNA (By
CC similarity). May play a role in plastid transcriptional regulation.
CC {ECO:0000250}.
CC -!- SUBUNIT: Part of the 50S ribosomal subunit. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=1;
CC Comment=A number of isoforms are produced. According to EST
CC sequences.;
CC Name=1;
CC IsoId=O50061-1; Sequence=Displayed;
CC -!- SIMILARITY: Belongs to the universal ribosomal protein uL4 family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAF79563.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; Y14565; CAA74894.1; -; mRNA.
DR EMBL; Y14566; CAA74895.1; -; Genomic_DNA.
DR EMBL; AC022464; AAF79563.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002684; AEE28106.1; -; Genomic_DNA.
DR EMBL; AY063889; AAL36245.1; -; mRNA.
DR EMBL; AY117317; AAM51392.1; -; mRNA.
DR EMBL; AY085081; AAM61637.1; -; mRNA.
DR PIR; D86208; D86208.
DR RefSeq; NP_001030985.1; NM_001035908.2.
DR RefSeq; NP_001030986.1; NM_001035909.1.
DR RefSeq; NP_563786.1; NM_100606.4. [O50061-1]
DR AlphaFoldDB; O50061; -.
DR SMR; O50061; -.
DR BioGRID; 22484; 8.
DR IntAct; O50061; 1.
DR STRING; 3702.AT1G07320.1; -.
DR iPTMnet; O50061; -.
DR PaxDb; O50061; -.
DR PRIDE; O50061; -.
DR ProteomicsDB; 234712; -. [O50061-1]
DR EnsemblPlants; AT1G07320.1; AT1G07320.1; AT1G07320. [O50061-1]
DR GeneID; 837243; -.
DR Gramene; AT1G07320.1; AT1G07320.1; AT1G07320. [O50061-1]
DR KEGG; ath:AT1G07320; -.
DR Araport; AT1G07320; -.
DR TAIR; locus:2025107; AT1G07320.
DR eggNOG; KOG1624; Eukaryota.
DR InParanoid; O50061; -.
DR PhylomeDB; O50061; -.
DR PRO; PR:O50061; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; O50061; baseline and differential.
DR Genevisible; O50061; AT.
DR GO; GO:0009507; C:chloroplast; HDA:TAIR.
DR GO; GO:0009570; C:chloroplast stroma; HDA:TAIR.
DR GO; GO:0009535; C:chloroplast thylakoid membrane; HDA:TAIR.
DR GO; GO:0022626; C:cytosolic ribosome; HDA:TAIR.
DR GO; GO:0005576; C:extracellular region; HDA:TAIR.
DR GO; GO:0005634; C:nucleus; HDA:TAIR.
DR GO; GO:0000311; C:plastid large ribosomal subunit; TAS:TAIR.
DR GO; GO:0009547; C:plastid ribosome; IDA:TAIR.
DR GO; GO:0009579; C:thylakoid; HDA:TAIR.
DR GO; GO:0003729; F:mRNA binding; IDA:TAIR.
DR GO; GO:0008266; F:poly(U) RNA binding; IDA:TAIR.
DR GO; GO:0019843; F:rRNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003735; F:structural constituent of ribosome; ISS:TAIR.
DR GO; GO:0006412; P:translation; TAS:TAIR.
DR Gene3D; 3.40.1370.10; -; 1.
DR HAMAP; MF_01328_B; Ribosomal_L4_B; 1.
DR InterPro; IPR002136; Ribosomal_L4/L1e.
DR InterPro; IPR023574; Ribosomal_L4_dom_sf.
DR InterPro; IPR013005; Ribosomal_uL4/L1e.
DR PANTHER; PTHR10746; PTHR10746; 1.
DR Pfam; PF00573; Ribosomal_L4; 1.
DR SUPFAM; SSF52166; SSF52166; 1.
DR TIGRFAMs; TIGR03953; rplD_bact; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Chloroplast; Plastid; Reference proteome;
KW Ribonucleoprotein; Ribosomal protein; RNA-binding; rRNA-binding;
KW Transit peptide.
FT TRANSIT 1..49
FT /note="Chloroplast"
FT /evidence="ECO:0000305"
FT CHAIN 50..282
FT /note="50S ribosomal protein L4, chloroplastic"
FT /id="PRO_0000030538"
FT REGION 106..138
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 192
FT /note="F -> L (in Ref. 1; CAA74894/CAA74895)"
FT /evidence="ECO:0000305"
FT CONFLICT 207
FT /note="A -> P (in Ref. 5; AAM61637)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 282 AA; 30559 MW; 1479CC1CEA75F29C CRC64;
MASSATAPNS LSFFSSSLFL SSSHQIPKTY ISVSKLGSGR VSKPLSVSSQ LATLPILSFE
GEKVGETYLD LKAAPEDTAR AVVHRAIVTD LNNKRRGTAS TLTRGEVRGG GIKPYSQKKT
GHARRGSQRT PLRPGGGVVF GPRPKDWSIK INRKEKKLAI STALSSAASA EGGAIVVEEF
GEKFEKPKTK DFLAAMQRWG LDPKEKAMFL MIDVDENVAK SSRNIGTLRM LTPRTLNLFD
ILNADKLVLT PAAVEFLNAR YGVDAVEEED DDEDETEGSE EA
