RK6_SPIOL
ID RK6_SPIOL Reviewed; 220 AA.
AC P82193; A0A0K9R4N9;
DT 19-SEP-2006, integrated into UniProtKB/Swiss-Prot.
DT 12-APR-2017, sequence version 2.
DT 25-MAY-2022, entry version 78.
DE RecName: Full=50S ribosomal protein L6, chloroplastic {ECO:0000303|PubMed:10874046};
DE AltName: Full=Chloroplastic large ribosomal subunit protein uL6c {ECO:0000303|PubMed:28007896};
DE Flags: Precursor;
GN Name=RPL6; ORFNames=SOVF_107200;
OS Spinacia oleracea (Spinach).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC Caryophyllales; Chenopodiaceae; Chenopodioideae; Anserineae; Spinacia.
OX NCBI_TaxID=3562;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Viroflay; TISSUE=Leaf;
RX PubMed=24352233; DOI=10.1038/nature12817;
RA Dohm J.C., Minoche A.E., Holtgraewe D., Capella-Gutierrez S.,
RA Zakrzewski F., Tafer H., Rupp O., Soerensen T.R., Stracke R., Reinhardt R.,
RA Goesmann A., Kraft T., Schulz B., Stadler P.F., Schmidt T., Gabaldon T.,
RA Lehrach H., Weisshaar B., Himmelbauer H.;
RT "The genome of the recently domesticated crop plant sugar beet (Beta
RT vulgaris).";
RL Nature 505:546-549(2014).
RN [2]
RP PROTEIN SEQUENCE OF 39-68, SUBUNIT, SUBCELLULAR LOCATION, AND MASS
RP SPECTROMETRY.
RC STRAIN=cv. Alwaro; TISSUE=Leaf;
RX PubMed=10874046; DOI=10.1074/jbc.m005012200;
RA Yamaguchi K., Subramanian A.R.;
RT "The plastid ribosomal proteins. Identification of all the proteins in the
RT 50S subunit of an organelle ribosome (chloroplast).";
RL J. Biol. Chem. 275:28466-28482(2000).
RN [3]
RP STRUCTURE BY ELECTRON MICROSCOPY (9.4 ANGSTROMS).
RX PubMed=18042701; DOI=10.1073/pnas.0709856104;
RA Sharma M.R., Wilson D.N., Datta P.P., Barat C., Schluenzen F., Fucini P.,
RA Agrawal R.K.;
RT "Cryo-EM study of the spinach chloroplast ribosome reveals the structural
RT and functional roles of plastid-specific ribosomal proteins.";
RL Proc. Natl. Acad. Sci. U.S.A. 104:19315-19320(2007).
RN [4]
RP STRUCTURE BY ELECTRON MICROSCOPY (3.50 ANGSTROMS).
RX PubMed=27762343; DOI=10.1038/srep35793;
RA Ahmed T., Yin Z., Bhushan S.;
RT "Cryo-EM structure of the large subunit of the spinach chloroplast
RT ribosome.";
RL Sci. Rep. 6:35793-35793(2016).
RN [5]
RP STRUCTURE BY ELECTRON MICROSCOPY (3.25 ANGSTROMS), SUBUNIT, AND SUBCELLULAR
RP LOCATION.
RX PubMed=28007896; DOI=10.15252/embj.201695959;
RA Bieri P., Leibundgut M., Saurer M., Boehringer D., Ban N.;
RT "The complete structure of the chloroplast 70S ribosome in complex with
RT translation factor pY.";
RL EMBO J. 36:475-486(2017).
CC -!- FUNCTION: Component of the chloroplast ribosome (chloro-ribosome), a
CC dedicated translation machinery responsible for the synthesis of
CC chloroplast genome-encoded proteins, including proteins of the
CC transcription and translation machinery and components of the
CC photosynthetic apparatus. {ECO:0000305|PubMed:10874046,
CC ECO:0000305|PubMed:28007896}.
CC -!- SUBUNIT: Component of the chloroplast large ribosomal subunit (LSU).
CC Mature 70S chloroplast ribosomes of higher plants consist of a small
CC (30S) and a large (50S) subunit. The 30S small subunit contains 1
CC molecule of ribosomal RNA (16S rRNA) and 24 different proteins. The 50S
CC large subunit contains 3 rRNA molecules (23S, 5S and 4.5S rRNA) and 33
CC different proteins. {ECO:0000269|PubMed:10874046,
CC ECO:0000269|PubMed:28007896}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast
CC {ECO:0000269|PubMed:10874046, ECO:0000269|PubMed:28007896}.
CC -!- MASS SPECTROMETRY: Mass=20224.0; Method=Electrospray;
CC Evidence={ECO:0000269|PubMed:10874046};
CC -!- SIMILARITY: Belongs to the universal ribosomal protein uL6 family.
CC {ECO:0000255}.
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DR EMBL; KQ150353; KNA14466.1; -; Genomic_DNA.
DR PDB; 4V61; EM; 9.40 A; I=39-68.
DR PDB; 5H1S; EM; 3.50 A; I=39-220.
DR PDB; 5MLC; EM; 3.90 A; H=1-220.
DR PDB; 5MMI; EM; 3.25 A; G=1-220.
DR PDB; 5MMM; EM; 3.40 A; G=1-220.
DR PDB; 5X8P; EM; 3.40 A; G=39-220.
DR PDB; 5X8T; EM; 3.30 A; G=39-220.
DR PDB; 6ERI; EM; 3.00 A; AG=44-215.
DR PDBsum; 4V61; -.
DR PDBsum; 5H1S; -.
DR PDBsum; 5MLC; -.
DR PDBsum; 5MMI; -.
DR PDBsum; 5MMM; -.
DR PDBsum; 5X8P; -.
DR PDBsum; 5X8T; -.
DR PDBsum; 6ERI; -.
DR AlphaFoldDB; P82193; -.
DR SMR; P82193; -.
DR IntAct; P82193; 1.
DR STRING; 3562.P82193; -.
DR OrthoDB; 1509998at2759; -.
DR Proteomes; UP000054095; Unassembled WGS sequence.
DR GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR GO; GO:0005840; C:ribosome; IEA:UniProtKB-KW.
DR GO; GO:0019843; F:rRNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003735; F:structural constituent of ribosome; IBA:GO_Central.
DR GO; GO:0006412; P:translation; IEA:InterPro.
DR Gene3D; 3.90.930.12; -; 2.
DR HAMAP; MF_01365_B; Ribosomal_L6_B; 1.
DR InterPro; IPR000702; Ribosomal_L6.
DR InterPro; IPR020040; Ribosomal_L6_a/b-dom.
DR InterPro; IPR036789; Ribosomal_L6_a/b-dom_sf.
DR InterPro; IPR019906; Ribosomal_L6_bac-type.
DR InterPro; IPR002358; Ribosomal_L6_CS.
DR PANTHER; PTHR11655; PTHR11655; 1.
DR Pfam; PF00347; Ribosomal_L6; 2.
DR PRINTS; PR00059; RIBOSOMALL6.
DR SUPFAM; SSF56053; SSF56053; 2.
DR TIGRFAMs; TIGR03654; L6_bact; 1.
DR PROSITE; PS00525; RIBOSOMAL_L6_1; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Chloroplast; Direct protein sequencing; Plastid;
KW Reference proteome; Ribonucleoprotein; Ribosomal protein; RNA-binding;
KW rRNA-binding; Transit peptide.
FT TRANSIT 1..38
FT /note="Chloroplast"
FT /evidence="ECO:0000269|PubMed:10874046"
FT CHAIN 39..220
FT /note="50S ribosomal protein L6, chloroplastic"
FT /id="PRO_0000249859"
FT CONFLICT 52
FT /note="Missing (in Ref. 2; AA sequence)"
FT /evidence="ECO:0000305"
FT HELIX 42..45
FT /evidence="ECO:0007829|PDB:5MMI"
FT STRAND 55..59
FT /evidence="ECO:0007829|PDB:5MMI"
FT STRAND 62..67
FT /evidence="ECO:0007829|PDB:5MMI"
FT STRAND 70..75
FT /evidence="ECO:0007829|PDB:5MMI"
FT STRAND 78..84
FT /evidence="ECO:0007829|PDB:5MMI"
FT STRAND 86..96
FT /evidence="ECO:0007829|PDB:5MMI"
FT HELIX 99..120
FT /evidence="ECO:0007829|PDB:5MMI"
FT STRAND 123..131
FT /evidence="ECO:0007829|PDB:5MMI"
FT STRAND 135..139
FT /evidence="ECO:0007829|PDB:5MMI"
FT STRAND 142..151
FT /evidence="ECO:0007829|PDB:5MMI"
FT STRAND 153..156
FT /evidence="ECO:0007829|PDB:5MMI"
FT STRAND 162..166
FT /evidence="ECO:0007829|PDB:5MMI"
FT TURN 167..169
FT /evidence="ECO:0007829|PDB:5MMI"
FT STRAND 170..176
FT /evidence="ECO:0007829|PDB:5MMI"
FT HELIX 178..190
FT /evidence="ECO:0007829|PDB:5MMI"
FT TURN 196..198
FT /evidence="ECO:0007829|PDB:5MMI"
FT STRAND 201..206
FT /evidence="ECO:0007829|PDB:5MMI"
SQ SEQUENCE 220 AA; 24474 MW; 2E0BB3403003424E CRC64;
MSLPLPSHMK SVFLGMKVEI STSVPVTRIG FWRKSVDCKE SRIGKQPITV PANVAIAMEG
QDLKVKGPLG ELSITYPREV LVEKQESGFL RVRKAVETRR ANQMHGLFRT LTDNMVVGVS
KGFEKKLQLV GVGYRATVEG KDLILSLGFS HPVRMAIPDE LQVKVEENTK VTVSGRDKSV
VGQFAATIRS WRPPEPYKGK GVRYVDEVVR RKEGKAGKKK
