RKPI_RHIME
ID RKPI_RHIME Reviewed; 538 AA.
AC Q52938;
DT 30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT 30-MAY-2000, sequence version 2.
DT 25-MAY-2022, entry version 110.
DE RecName: Full=Capsular polysaccharide biosynthesis protein RkpI;
GN Name=rkpI; OrderedLocusNames=R00574; ORFNames=SMc02270;
OS Rhizobium meliloti (strain 1021) (Ensifer meliloti) (Sinorhizobium
OS meliloti).
OC Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC Rhizobiaceae; Sinorhizobium/Ensifer group; Sinorhizobium.
OX NCBI_TaxID=266834;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=41;
RX PubMed=9079896; DOI=10.1128/jb.179.7.2132-2140.1997;
RA Kiss E., Reuhs B.L., Kim J.S., Kereszt A., Petrovics G., Putnoky P.,
RA Dusha I., Carlson R.W., Kondorosi A.;
RT "The rkpGHI and -J genes are involved in capsular polysaccharide production
RT by Rhizobium meliloti.";
RL J. Bacteriol. 179:2132-2140(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=1021;
RX PubMed=11481430; DOI=10.1073/pnas.161294398;
RA Capela D., Barloy-Hubler F., Gouzy J., Bothe G., Ampe F., Batut J.,
RA Boistard P., Becker A., Boutry M., Cadieu E., Dreano S., Gloux S.,
RA Godrie T., Goffeau A., Kahn D., Kiss E., Lelaure V., Masuy D., Pohl T.,
RA Portetelle D., Puehler A., Purnelle B., Ramsperger U., Renard C.,
RA Thebault P., Vandenbol M., Weidner S., Galibert F.;
RT "Analysis of the chromosome sequence of the legume symbiont Sinorhizobium
RT meliloti strain 1021.";
RL Proc. Natl. Acad. Sci. U.S.A. 98:9877-9882(2001).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=1021;
RX PubMed=11474104; DOI=10.1126/science.1060966;
RA Galibert F., Finan T.M., Long S.R., Puehler A., Abola P., Ampe F.,
RA Barloy-Hubler F., Barnett M.J., Becker A., Boistard P., Bothe G.,
RA Boutry M., Bowser L., Buhrmester J., Cadieu E., Capela D., Chain P.,
RA Cowie A., Davis R.W., Dreano S., Federspiel N.A., Fisher R.F., Gloux S.,
RA Godrie T., Goffeau A., Golding B., Gouzy J., Gurjal M., Hernandez-Lucas I.,
RA Hong A., Huizar L., Hyman R.W., Jones T., Kahn D., Kahn M.L., Kalman S.,
RA Keating D.H., Kiss E., Komp C., Lelaure V., Masuy D., Palm C., Peck M.C.,
RA Pohl T.M., Portetelle D., Purnelle B., Ramsperger U., Surzycki R.,
RA Thebault P., Vandenbol M., Vorhoelter F.J., Weidner S., Wells D.H.,
RA Wong K., Yeh K.-C., Batut J.;
RT "The composite genome of the legume symbiont Sinorhizobium meliloti.";
RL Science 293:668-672(2001).
CC -!- FUNCTION: Involved in antigen K (capsular polysaccharide) biosynthesis.
CC -!- PATHWAY: Capsule biogenesis; capsule polysaccharide biosynthesis.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
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DR EMBL; X64131; CAA45491.1; -; Genomic_DNA.
DR EMBL; AL591688; CAC45146.1; -; Genomic_DNA.
DR RefSeq; NP_384680.1; NC_003047.1.
DR RefSeq; WP_010968671.1; NC_003047.1.
DR AlphaFoldDB; Q52938; -.
DR SMR; Q52938; -.
DR STRING; 266834.SMc02270; -.
DR EnsemblBacteria; CAC45146; CAC45146; SMc02270.
DR GeneID; 61602047; -.
DR KEGG; sme:SMc02270; -.
DR PATRIC; fig|266834.11.peg.1950; -.
DR eggNOG; COG1368; Bacteria.
DR HOGENOM; CLU_023230_0_0_5; -.
DR OMA; NTMRTEF; -.
DR UniPathway; UPA00934; -.
DR Proteomes; UP000001976; Chromosome.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0008484; F:sulfuric ester hydrolase activity; IEA:InterPro.
DR GO; GO:0045227; P:capsule polysaccharide biosynthetic process; IEA:UniProtKB-UniPathway.
DR Gene3D; 3.40.720.10; -; 1.
DR InterPro; IPR017850; Alkaline_phosphatase_core_sf.
DR InterPro; IPR000917; Sulfatase_N.
DR Pfam; PF00884; Sulfatase; 1.
DR SUPFAM; SSF53649; SSF53649; 1.
PE 4: Predicted;
KW Capsule biogenesis/degradation; Cell membrane; Membrane;
KW Reference proteome; Transmembrane; Transmembrane helix.
FT CHAIN 1..538
FT /note="Capsular polysaccharide biosynthesis protein RkpI"
FT /id="PRO_0000097358"
FT TRANSMEM 16..36
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 70..90
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 114..134
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 139..159
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 170..190
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 212..232
FT /note="Helical"
FT /evidence="ECO:0000255"
FT CONFLICT 64
FT /note="R -> H (in Ref. 1; CAA45491)"
FT /evidence="ECO:0000305"
FT CONFLICT 349
FT /note="Missing (in Ref. 1; CAA45491)"
FT /evidence="ECO:0000305"
FT CONFLICT 514
FT /note="A -> R (in Ref. 1; CAA45491)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 538 AA; 61187 MW; FCE04066220B1838 CRC64;
MKWLKGIGLA LIPFNLHDYP IALTLGCYLL SCAVIFYTDR FALPARERRK NAPKYGRNRD
RIDRLARLPV IALVFAGFFA ISWRPLYAAA GTMSFFIIFT GISRAKFKFI REPLVFSDIA
LVADVFKYKT IFYASSLNIV FWIVAFLYVF GVSALYMYFE PTILPERSRL FWVLVMVGIA
AGPWGLLFYG PVNRPTAALV QRLVKAINVK MNTVRFGTFA SVVFHFIIWL GVKRDKIVAE
LSEILRAAVH DLIGHEEAPL IIVWQSESFI DMRHFGVDSI KLPTVDRLRK QAVQWGRLSN
VFEGGYTLRT EFAVLSGLVP DDIHVDASYP YLRAAHYADI VWPGKLKRAG WRTHFIHPYD
RTFFLRHKAM PLLGFEKLTM LDAFDHKPER DGLYVSDATL AARVLAEVEK LPEEESGFFF
VASMANHGPW EPGRVGTLTN PVDIYLAILE QSDAALKQLI DGLNKLDRPV WFVFYGDHAP
LLKSFADPFP DPRTDYFIVP LAKARAAAHS PKRAKDEDPW NLLGSMLKHA NLHKDALQ
