RL10_THEMA
ID RL10_THEMA Reviewed; 179 AA.
AC P29394;
DT 01-DEC-1992, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1996, sequence version 2.
DT 25-MAY-2022, entry version 130.
DE RecName: Full=50S ribosomal protein L10;
GN Name=rplJ; OrderedLocusNames=TM_0456;
OS Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826
OS / MSB8).
OC Bacteria; Thermotogae; Thermotogales; Thermotogaceae; Thermotoga.
OX NCBI_TaxID=243274;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8;
RX PubMed=1429627; DOI=10.1016/s0021-9258(18)50016-8;
RA Liao D., Dennis P.P.;
RT "The organization and expression of essential transcription translation
RT component genes in the extremely thermophilic eubacterium Thermotoga
RT maritima.";
RL J. Biol. Chem. 267:22787-22797(1992).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8;
RX PubMed=10360571; DOI=10.1038/20601;
RA Nelson K.E., Clayton R.A., Gill S.R., Gwinn M.L., Dodson R.J., Haft D.H.,
RA Hickey E.K., Peterson J.D., Nelson W.C., Ketchum K.A., McDonald L.A.,
RA Utterback T.R., Malek J.A., Linher K.D., Garrett M.M., Stewart A.M.,
RA Cotton M.D., Pratt M.S., Phillips C.A., Richardson D.L., Heidelberg J.F.,
RA Sutton G.G., Fleischmann R.D., Eisen J.A., White O., Salzberg S.L.,
RA Smith H.O., Venter J.C., Fraser C.M.;
RT "Evidence for lateral gene transfer between Archaea and Bacteria from
RT genome sequence of Thermotoga maritima.";
RL Nature 399:323-329(1999).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 45-179.
RX PubMed=8177738; DOI=10.1093/nar/21.21.4904;
RA Palm P., Schleper C., Arnold-Ammer I., Holz I., Meier T., Lottspeich F.,
RA Zillig W.;
RT "The DNA-dependent RNA-polymerase of Thermotoga maritima; characterisation
RT of the enzyme and the DNA-sequence of the genes for the large subunits.";
RL Nucleic Acids Res. 21:4904-4908(1993).
RN [4]
RP SUBUNIT, STOICHIOMETRY, AND MASS SPECTROMETRY.
RX PubMed=15923259; DOI=10.1073/pnas.0502193102;
RA Ilag L.L., Videler H., McKay A.R., Sobott F., Fucini P., Nierhaus K.H.,
RA Robinson C.V.;
RT "Heptameric (L12)6/L10 rather than canonical pentameric complexes are found
RT by tandem MS of intact ribosomes from thermophilic bacteria.";
RL Proc. Natl. Acad. Sci. U.S.A. 102:8192-8197(2005).
RN [5]
RP X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS), INTERACTION WITH L7/L12 (RPLL), AND
RP SUBUNIT.
RX PubMed=15989950; DOI=10.1016/j.cell.2005.04.015;
RA Diaconu M., Kothe U., Schlunzen F., Fischer N., Harms J.M.,
RA Tonevitsky A.G., Stark H., Rodnina M.V., Wahl M.C.;
RT "Structural basis for the function of the ribosomal L7/12 stalk in factor
RT binding and GTPase activation.";
RL Cell 121:991-1004(2005).
CC -!- FUNCTION: Forms part of the ribosomal stalk, playing a central role in
CC the interaction of the ribosome with GTP-bound translation factors
CC (such as IF-2, EF-Tu, EF-G and RF3). {ECO:0000305}.
CC -!- SUBUNIT: Part of the ribosomal stalk of the 50S ribosomal subunit. The
CC N-terminus interacts with L11 and 23S rRNA to form the base of the
CC stalk. The C-terminus forms an elongated spine to which 3 L12 dimers
CC bind in a sequential fashion forming a heptameric L10(L12)2(L12)2(L12)2
CC complex. {ECO:0000269|PubMed:15923259, ECO:0000269|PubMed:15989950}.
CC -!- MASS SPECTROMETRY: Mass=20335; Mass_error=3; Method=Electrospray;
CC Evidence={ECO:0000269|PubMed:15923259};
CC -!- SIMILARITY: Belongs to the universal ribosomal protein uL10 family.
CC {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; Z11839; CAA77861.1; -; Genomic_DNA.
DR EMBL; AE000512; AAD35539.1; -; Genomic_DNA.
DR EMBL; X72695; CAA51244.1; -; Genomic_DNA.
DR PIR; A72376; R5HG10.
DR RefSeq; NP_228266.1; NC_000853.1.
DR RefSeq; WP_004081510.1; NZ_CP011107.1.
DR PDB; 1ZAV; X-ray; 1.90 A; A=1-179.
DR PDB; 1ZAW; X-ray; 2.30 A; A=1-179.
DR PDB; 1ZAX; X-ray; 2.10 A; A=1-179.
DR PDBsum; 1ZAV; -.
DR PDBsum; 1ZAW; -.
DR PDBsum; 1ZAX; -.
DR AlphaFoldDB; P29394; -.
DR SMR; P29394; -.
DR STRING; 243274.THEMA_02410; -.
DR EnsemblBacteria; AAD35539; AAD35539; TM_0456.
DR KEGG; tma:TM0456; -.
DR eggNOG; COG0244; Bacteria.
DR InParanoid; P29394; -.
DR OMA; VRDQKQA; -.
DR OrthoDB; 1470978at2; -.
DR EvolutionaryTrace; P29394; -.
DR Proteomes; UP000008183; Chromosome.
DR GO; GO:0022625; C:cytosolic large ribosomal subunit; IBA:GO_Central.
DR GO; GO:0015934; C:large ribosomal subunit; IBA:GO_Central.
DR GO; GO:0070180; F:large ribosomal subunit rRNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003735; F:structural constituent of ribosome; IBA:GO_Central.
DR GO; GO:0006412; P:translation; IBA:GO_Central.
DR CDD; cd05797; Ribosomal_L10; 1.
DR Gene3D; 3.30.70.1730; -; 1.
DR HAMAP; MF_00362; Ribosomal_L10; 1.
DR InterPro; IPR022973; Ribosomal_L10.
DR InterPro; IPR043141; Ribosomal_L10-like_sf.
DR InterPro; IPR002363; Ribosomal_L10_eubac_CS.
DR InterPro; IPR001790; Ribosomal_L10P.
DR PANTHER; PTHR11560; PTHR11560; 1.
DR Pfam; PF00466; Ribosomal_L10; 1.
DR SUPFAM; SSF160369; SSF160369; 1.
DR PROSITE; PS01109; RIBOSOMAL_L10; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Reference proteome; Ribonucleoprotein; Ribosomal protein;
KW RNA-binding; rRNA-binding.
FT CHAIN 1..179
FT /note="50S ribosomal protein L10"
FT /id="PRO_0000154734"
FT REGION 137..179
FT /note="Binds L7/L12 dimers"
FT HELIX 4..18
FT /evidence="ECO:0007829|PDB:1ZAV"
FT STRAND 22..27
FT /evidence="ECO:0007829|PDB:1ZAV"
FT HELIX 34..48
FT /evidence="ECO:0007829|PDB:1ZAV"
FT STRAND 51..57
FT /evidence="ECO:0007829|PDB:1ZAV"
FT HELIX 59..68
FT /evidence="ECO:0007829|PDB:1ZAV"
FT HELIX 75..77
FT /evidence="ECO:0007829|PDB:1ZAV"
FT STRAND 79..90
FT /evidence="ECO:0007829|PDB:1ZAV"
FT HELIX 93..105
FT /evidence="ECO:0007829|PDB:1ZAV"
FT HELIX 110..112
FT /evidence="ECO:0007829|PDB:1ZAV"
FT STRAND 113..118
FT /evidence="ECO:0007829|PDB:1ZAV"
FT STRAND 121..124
FT /evidence="ECO:0007829|PDB:1ZAV"
FT HELIX 125..132
FT /evidence="ECO:0007829|PDB:1ZAV"
FT HELIX 137..175
FT /evidence="ECO:0007829|PDB:1ZAV"
SQ SEQUENCE 179 AA; 20308 MW; 497C5764A64FC7F9 CRC64;
MLTRQQKELI VKEMSEIFKK TSLILFADFL GFTVADLTEL RSRLREKYGD GARFRVVKNT
LLNLALKNAE YEGYEEFLKG PTAVLYVTEG DPVEAVKIIY NFYKDKKADL SRLKGGFLEG
KKFTAEEVEN IAKLPSKEEL YAMLVGRVKA PITGLVFALS GILRNLVYVL NAIKEKKSE
