RL13_BACSU
ID RL13_BACSU Reviewed; 145 AA.
AC P70974;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 16-JUN-2009, sequence version 2.
DT 03-AUG-2022, entry version 136.
DE RecName: Full=50S ribosomal protein L13 {ECO:0000255|HAMAP-Rule:MF_01366};
GN Name=rplM {ECO:0000255|HAMAP-Rule:MF_01366}; OrderedLocusNames=BSU01490;
OS Bacillus subtilis (strain 168).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX NCBI_TaxID=224308;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=168;
RX PubMed=8969501; DOI=10.1099/13500872-142-11-3039;
RA Yasumoto K., Liu H., Jeong S.M., Ohashi Y., Kakinuma S., Tanaka K.,
RA Kawamura F., Yoshikawa H., Takahashi H.;
RT "Sequence analysis of a 50 kb region between spo0H and rrnH on the Bacillus
RT subtilis chromosome.";
RL Microbiology 142:3039-3046(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=168;
RX PubMed=9384377; DOI=10.1038/36786;
RA Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA Yoshikawa H., Danchin A.;
RT "The complete genome sequence of the Gram-positive bacterium Bacillus
RT subtilis.";
RL Nature 390:249-256(1997).
RN [3]
RP SEQUENCE REVISION TO 16.
RX PubMed=19383706; DOI=10.1099/mic.0.027839-0;
RA Barbe V., Cruveiller S., Kunst F., Lenoble P., Meurice G., Sekowska A.,
RA Vallenet D., Wang T., Moszer I., Medigue C., Danchin A.;
RT "From a consortium sequence to a unified sequence: the Bacillus subtilis
RT 168 reference genome a decade later.";
RL Microbiology 155:1758-1775(2009).
RN [4]
RP PROTEIN SEQUENCE OF 1-12, SUBUNIT, AND BINDING TO OBG.
RC STRAIN=PY22;
RX PubMed=10781545; DOI=10.1128/jb.182.10.2771-2777.2000;
RA Scott J.M., Ju J., Mitchell T., Haldenwang W.G.;
RT "The Bacillus subtilis GTP binding protein obg and regulators of the
RT sigma(B) stress response transcription factor cofractionate with
RT ribosomes.";
RL J. Bacteriol. 182:2771-2777(2000).
RN [5] {ECO:0007744|PDB:6HA1, ECO:0007744|PDB:6HA8}
RP STRUCTURE BY ELECTRON MICROSCOPY (3.10 ANGSTROMS) OF 1-145 WITH AND WITHOUT
RP VIRGINIAMYCIN M.
RX PubMed=30126986; DOI=10.1073/pnas.1808535115;
RA Crowe-McAuliffe C., Graf M., Huter P., Takada H., Abdelshahid M.,
RA Novacek J., Murina V., Atkinson G.C., Hauryliuk V., Wilson D.N.;
RT "Structural basis for antibiotic resistance mediated by the Bacillus
RT subtilis ABCF ATPase VmlR.";
RL Proc. Natl. Acad. Sci. U.S.A. 115:8978-8983(2018).
CC -!- FUNCTION: This protein is one of the early assembly proteins of the 50S
CC ribosomal subunit, although it is not seen to bind rRNA by itself. It
CC is important during the early stages of 50S assembly.
CC {ECO:0000255|HAMAP-Rule:MF_01366}.
CC -!- SUBUNIT: Part of the 50S ribosomal subunit (PubMed:30126986). Binds to
CC Obg (AC P20964) (PubMed:10781545). {ECO:0000269|PubMed:10781545,
CC ECO:0000269|PubMed:30126986}.
CC -!- SIMILARITY: Belongs to the universal ribosomal protein uL13 family.
CC {ECO:0000255|HAMAP-Rule:MF_01366}.
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DR EMBL; D64126; BAA10988.1; -; Genomic_DNA.
DR EMBL; AL009126; CAB11925.2; -; Genomic_DNA.
DR PIR; G69695; G69695.
DR RefSeq; NP_388030.2; NC_000964.3.
DR RefSeq; WP_003241966.1; NZ_JNCM01000029.1.
DR PDB; 3J3V; EM; 13.30 A; J=1-145.
DR PDB; 3J3W; EM; 10.70 A; J=1-145.
DR PDB; 3J9W; EM; 3.90 A; BM=1-145.
DR PDB; 5NJT; EM; 3.80 A; c=4-145.
DR PDB; 6HA1; EM; 3.10 A; J=1-145.
DR PDB; 6HA8; EM; 3.50 A; J=1-145.
DR PDB; 6HTQ; EM; 4.50 A; J=4-145.
DR PDB; 6PPF; EM; 3.40 A; J=1-145.
DR PDB; 6PPK; EM; 4.40 A; J=1-145.
DR PDB; 6PVK; EM; 3.40 A; J=1-145.
DR PDB; 6TNN; EM; 3.07 A; c=1-145.
DR PDB; 6TPQ; EM; 3.07 A; c=1-145.
DR PDB; 7AQC; EM; 2.99 A; J=1-145.
DR PDB; 7AQD; EM; 3.10 A; J=1-145.
DR PDB; 7AS8; EM; 2.90 A; N=1-145.
DR PDB; 7AS9; EM; 3.50 A; N=1-145.
DR PDB; 7O5B; EM; 3.33 A; e=1-145.
DR PDB; 7OPE; EM; 3.20 A; N=1-145.
DR PDB; 7QV1; EM; 3.50 A; J=1-145.
DR PDB; 7QV2; EM; 3.50 A; J=1-145.
DR PDB; 7QV3; EM; 5.14 A; J=1-145.
DR PDBsum; 3J3V; -.
DR PDBsum; 3J3W; -.
DR PDBsum; 3J9W; -.
DR PDBsum; 5NJT; -.
DR PDBsum; 6HA1; -.
DR PDBsum; 6HA8; -.
DR PDBsum; 6HTQ; -.
DR PDBsum; 6PPF; -.
DR PDBsum; 6PPK; -.
DR PDBsum; 6PVK; -.
DR PDBsum; 6TNN; -.
DR PDBsum; 6TPQ; -.
DR PDBsum; 7AQC; -.
DR PDBsum; 7AQD; -.
DR PDBsum; 7AS8; -.
DR PDBsum; 7AS9; -.
DR PDBsum; 7O5B; -.
DR PDBsum; 7OPE; -.
DR PDBsum; 7QV1; -.
DR PDBsum; 7QV2; -.
DR PDBsum; 7QV3; -.
DR AlphaFoldDB; P70974; -.
DR SMR; P70974; -.
DR IntAct; P70974; 1.
DR STRING; 224308.BSU01490; -.
DR jPOST; P70974; -.
DR PaxDb; P70974; -.
DR PRIDE; P70974; -.
DR EnsemblBacteria; CAB11925; CAB11925; BSU_01490.
DR GeneID; 50135109; -.
DR GeneID; 938915; -.
DR KEGG; bsu:BSU01490; -.
DR PATRIC; fig|224308.179.peg.153; -.
DR eggNOG; COG0102; Bacteria.
DR InParanoid; P70974; -.
DR OMA; DFVVIIN; -.
DR PhylomeDB; P70974; -.
DR BioCyc; BSUB:BSU01490-MON; -.
DR PRO; PR:P70974; -.
DR Proteomes; UP000001570; Chromosome.
DR GO; GO:0022625; C:cytosolic large ribosomal subunit; IBA:GO_Central.
DR GO; GO:0005840; C:ribosome; IBA:GO_Central.
DR GO; GO:0003729; F:mRNA binding; IBA:GO_Central.
DR GO; GO:0003735; F:structural constituent of ribosome; IBA:GO_Central.
DR GO; GO:0017148; P:negative regulation of translation; IBA:GO_Central.
DR GO; GO:0006412; P:translation; IEA:UniProtKB-UniRule.
DR CDD; cd00392; Ribosomal_L13; 1.
DR Gene3D; 3.90.1180.10; -; 1.
DR HAMAP; MF_01366; Ribosomal_L13; 1.
DR InterPro; IPR005822; Ribosomal_L13.
DR InterPro; IPR005823; Ribosomal_L13_bac-type.
DR InterPro; IPR023563; Ribosomal_L13_CS.
DR InterPro; IPR036899; Ribosomal_L13_sf.
DR PANTHER; PTHR11545; PTHR11545; 1.
DR Pfam; PF00572; Ribosomal_L13; 1.
DR PIRSF; PIRSF002181; Ribosomal_L13; 1.
DR SUPFAM; SSF52161; SSF52161; 1.
DR TIGRFAMs; TIGR01066; rplM_bact; 1.
DR PROSITE; PS00783; RIBOSOMAL_L13; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Direct protein sequencing; Reference proteome;
KW Ribonucleoprotein; Ribosomal protein.
FT CHAIN 1..145
FT /note="50S ribosomal protein L13"
FT /id="PRO_0000133725"
FT CONFLICT 16
FT /note="W -> C (in Ref. 1; BAA10988)"
FT /evidence="ECO:0000305"
FT STRAND 16..20
FT /evidence="ECO:0007829|PDB:7AS8"
FT STRAND 22..24
FT /evidence="ECO:0007829|PDB:7AQC"
FT HELIX 26..38
FT /evidence="ECO:0007829|PDB:7AS8"
FT TURN 39..41
FT /evidence="ECO:0007829|PDB:7AS8"
FT STRAND 42..44
FT /evidence="ECO:0007829|PDB:7AS8"
FT TURN 47..49
FT /evidence="ECO:0007829|PDB:7AQC"
FT STRAND 54..58
FT /evidence="ECO:0007829|PDB:7AS8"
FT HELIX 60..62
FT /evidence="ECO:0007829|PDB:7AS8"
FT HELIX 69..72
FT /evidence="ECO:0007829|PDB:7AS8"
FT STRAND 74..78
FT /evidence="ECO:0007829|PDB:7AS8"
FT STRAND 85..89
FT /evidence="ECO:0007829|PDB:7AS8"
FT HELIX 90..96
FT /evidence="ECO:0007829|PDB:7AS8"
FT HELIX 98..109
FT /evidence="ECO:0007829|PDB:7AS8"
FT HELIX 114..120
FT /evidence="ECO:0007829|PDB:7AS8"
FT STRAND 123..125
FT /evidence="ECO:0007829|PDB:7AS8"
FT STRAND 127..129
FT /evidence="ECO:0007829|PDB:6TNN"
FT HELIX 134..136
FT /evidence="ECO:0007829|PDB:7AQC"
SQ SEQUENCE 145 AA; 16375 MW; 28563533DF244606 CRC64;
MRTTPMANAS TIERKWLVVD AAGKTLGRLS SEVAAILRGK HKPTYTPHVD TGDHVIIINA
EKIELTGKKL TDKIYYRHTQ HPGGLKSRTA LEMRTNYPEK MLELAIKGML PKGSLGRQMF
KKLNVYRGSE HPHEAQKPEV YELRG
