RL14_BACSU
ID RL14_BACSU Reviewed; 122 AA.
AC P12875;
DT 01-OCT-1989, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1989, sequence version 1.
DT 03-AUG-2022, entry version 144.
DE RecName: Full=50S ribosomal protein L14 {ECO:0000255|HAMAP-Rule:MF_01367};
GN Name=rplN {ECO:0000255|HAMAP-Rule:MF_01367}; OrderedLocusNames=BSU01260;
OS Bacillus subtilis (strain 168).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX NCBI_TaxID=224308;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=168;
RX PubMed=2508062; DOI=10.1093/nar/17.18.7469;
RA Henkin T.M., Moon S.H., Mattheakis L.C., Nomura M.;
RT "Cloning and analysis of the spc ribosomal protein operon of Bacillus
RT subtilis: comparison with the spc operon of Escherichia coli.";
RL Nucleic Acids Res. 17:7469-7486(1989).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=168 / Marburg / ATCC 6051 / DSM 10 / JCM 1465 / NBRC 13719 / NCIMB
RC 3610 / NRRL NRS-744 / VKM B-501;
RX PubMed=8635744; DOI=10.1016/0378-1119(95)00757-1;
RA Suh J.-W., Boylan S.A., Oh S.H., Price C.W.;
RT "Genetic and transcriptional organization of the Bacillus subtilis spc-
RT alpha region.";
RL Gene 169:17-23(1996).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=168;
RX PubMed=9384377; DOI=10.1038/36786;
RA Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA Yoshikawa H., Danchin A.;
RT "The complete genome sequence of the Gram-positive bacterium Bacillus
RT subtilis.";
RL Nature 390:249-256(1997).
RN [4] {ECO:0007744|PDB:6HA1, ECO:0007744|PDB:6HA8}
RP STRUCTURE BY ELECTRON MICROSCOPY (3.10 ANGSTROMS) OF 1-122 WITH AND WITHOUT
RP VIRGINIAMYCIN M.
RX PubMed=30126986; DOI=10.1073/pnas.1808535115;
RA Crowe-McAuliffe C., Graf M., Huter P., Takada H., Abdelshahid M.,
RA Novacek J., Murina V., Atkinson G.C., Hauryliuk V., Wilson D.N.;
RT "Structural basis for antibiotic resistance mediated by the Bacillus
RT subtilis ABCF ATPase VmlR.";
RL Proc. Natl. Acad. Sci. U.S.A. 115:8978-8983(2018).
CC -!- FUNCTION: Binds to 23S rRNA. Forms part of two intersubunit bridges in
CC the 70S ribosome. {ECO:0000255|HAMAP-Rule:MF_01367}.
CC -!- SUBUNIT: Part of the 50S ribosomal subunit (PubMed:30126986). Forms a
CC cluster with proteins L3 and L19. In the 70S ribosome, L14 and L19
CC interact and together make contacts with the 16S rRNA in bridges B5 and
CC B8 (By similarity). {ECO:0000255|HAMAP-Rule:MF_01367,
CC ECO:0000269|PubMed:30126986}.
CC -!- SIMILARITY: Belongs to the universal ribosomal protein uL14 family.
CC {ECO:0000255|HAMAP-Rule:MF_01367}.
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DR EMBL; X15664; CAA33701.1; -; Genomic_DNA.
DR EMBL; L47971; AAB06809.1; -; Genomic_DNA.
DR EMBL; AL009126; CAB11902.1; -; Genomic_DNA.
DR PIR; S05992; R5BS4B.
DR RefSeq; NP_388007.1; NC_000964.3.
DR RefSeq; WP_003225805.1; NZ_JNCM01000029.1.
DR PDB; 3J3V; EM; 13.30 A; K=1-122.
DR PDB; 3J3W; EM; 10.70 A; K=1-122.
DR PDB; 3J9W; EM; 3.90 A; BN=1-122.
DR PDB; 5NJT; EM; 3.80 A; d=1-122.
DR PDB; 6HA1; EM; 3.10 A; K=1-122.
DR PDB; 6HA8; EM; 3.50 A; K=1-122.
DR PDB; 6HTQ; EM; 4.50 A; K=1-122.
DR PDB; 6PPF; EM; 3.40 A; K=1-122.
DR PDB; 6PPK; EM; 4.40 A; K=1-122.
DR PDB; 6PVK; EM; 3.40 A; K=1-122.
DR PDB; 6TNN; EM; 3.07 A; d=1-122.
DR PDB; 6TPQ; EM; 3.07 A; d=1-122.
DR PDB; 7AQC; EM; 2.99 A; K=1-122.
DR PDB; 7AQD; EM; 3.10 A; K=1-122.
DR PDB; 7AS8; EM; 2.90 A; O=1-122.
DR PDB; 7AS9; EM; 3.50 A; O=1-122.
DR PDB; 7O5B; EM; 3.33 A; f=1-122.
DR PDB; 7OPE; EM; 3.20 A; O=1-122.
DR PDB; 7QV1; EM; 3.50 A; K=1-122.
DR PDB; 7QV2; EM; 3.50 A; K=1-122.
DR PDB; 7QV3; EM; 5.14 A; K=1-122.
DR PDBsum; 3J3V; -.
DR PDBsum; 3J3W; -.
DR PDBsum; 3J9W; -.
DR PDBsum; 5NJT; -.
DR PDBsum; 6HA1; -.
DR PDBsum; 6HA8; -.
DR PDBsum; 6HTQ; -.
DR PDBsum; 6PPF; -.
DR PDBsum; 6PPK; -.
DR PDBsum; 6PVK; -.
DR PDBsum; 6TNN; -.
DR PDBsum; 6TPQ; -.
DR PDBsum; 7AQC; -.
DR PDBsum; 7AQD; -.
DR PDBsum; 7AS8; -.
DR PDBsum; 7AS9; -.
DR PDBsum; 7O5B; -.
DR PDBsum; 7OPE; -.
DR PDBsum; 7QV1; -.
DR PDBsum; 7QV2; -.
DR PDBsum; 7QV3; -.
DR AlphaFoldDB; P12875; -.
DR SMR; P12875; -.
DR IntAct; P12875; 1.
DR STRING; 224308.BSU01260; -.
DR jPOST; P12875; -.
DR PaxDb; P12875; -.
DR PRIDE; P12875; -.
DR EnsemblBacteria; CAB11902; CAB11902; BSU_01260.
DR GeneID; 50135133; -.
DR GeneID; 64301964; -.
DR GeneID; 935939; -.
DR KEGG; bsu:BSU01260; -.
DR PATRIC; fig|224308.179.peg.129; -.
DR eggNOG; COG0093; Bacteria.
DR InParanoid; P12875; -.
DR OMA; AKEVLCI; -.
DR PhylomeDB; P12875; -.
DR BioCyc; BSUB:BSU01260-MON; -.
DR PRO; PR:P12875; -.
DR Proteomes; UP000001570; Chromosome.
DR GO; GO:0022625; C:cytosolic large ribosomal subunit; IBA:GO_Central.
DR GO; GO:0070180; F:large ribosomal subunit rRNA binding; IBA:GO_Central.
DR GO; GO:0003735; F:structural constituent of ribosome; IBA:GO_Central.
DR GO; GO:0006412; P:translation; IEA:UniProtKB-UniRule.
DR Gene3D; 2.40.150.20; -; 1.
DR HAMAP; MF_01367; Ribosomal_L14; 1.
DR InterPro; IPR036853; Ribosomal_L14_sf.
DR InterPro; IPR000218; Ribosomal_L14P.
DR InterPro; IPR005745; Ribosomal_L14P_bac-type.
DR InterPro; IPR019972; Ribosomal_L14P_CS.
DR PANTHER; PTHR11761; PTHR11761; 1.
DR Pfam; PF00238; Ribosomal_L14; 1.
DR SMART; SM01374; Ribosomal_L14; 1.
DR SUPFAM; SSF50193; SSF50193; 1.
DR TIGRFAMs; TIGR01067; rplN_bact; 1.
DR PROSITE; PS00049; RIBOSOMAL_L14; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Reference proteome; Ribonucleoprotein; Ribosomal protein;
KW RNA-binding; rRNA-binding.
FT CHAIN 1..122
FT /note="50S ribosomal protein L14"
FT /id="PRO_0000128530"
FT STRAND 7..10
FT /evidence="ECO:0007829|PDB:7AS8"
FT STRAND 12..29
FT /evidence="ECO:0007829|PDB:7AS8"
FT STRAND 38..46
FT /evidence="ECO:0007829|PDB:7AS8"
FT STRAND 48..52
FT /evidence="ECO:0007829|PDB:7AS8"
FT STRAND 57..64
FT /evidence="ECO:0007829|PDB:7AS8"
FT STRAND 72..74
FT /evidence="ECO:0007829|PDB:6PPF"
FT STRAND 76..81
FT /evidence="ECO:0007829|PDB:7AS8"
FT STRAND 83..96
FT /evidence="ECO:0007829|PDB:7AS8"
FT STRAND 100..102
FT /evidence="ECO:0007829|PDB:7AS9"
FT HELIX 104..106
FT /evidence="ECO:0007829|PDB:7AS8"
FT TURN 107..110
FT /evidence="ECO:0007829|PDB:7AS8"
FT HELIX 112..117
FT /evidence="ECO:0007829|PDB:7AS8"
FT STRAND 119..121
FT /evidence="ECO:0007829|PDB:7AS9"
SQ SEQUENCE 122 AA; 13154 MW; 1C54AECFD7F87E72 CRC64;
MIQQETRLKV ADNSGAREVL TIKVLGGSGR KTANIGDVIV CTVKQATPGG VVKKGEVVKA
VIVRTKSGAR RSDGSYISFD ENACVIIRDD KSPRGTRIFG PVARELRENN FMKIVSLAPE
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