AAP_YEAST
ID AAP_YEAST Reviewed; 25 AA.
AC P08521; D6W301;
DT 01-AUG-1988, integrated into UniProtKB/Swiss-Prot.
DT 01-AUG-1988, sequence version 1.
DT 03-AUG-2022, entry version 115.
DE RecName: Full=Arginine attenuator peptide;
DE Short=AAP;
DE AltName: Full=CPA1 leader peptide;
GN OrderedLocusNames=YOR302W;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=3881260; DOI=10.1111/j.1432-1033.1985.tb08663.x;
RA Werner M., Feller A., Pierard A.;
RT "Nucleotide sequence of yeast gene CP A1 encoding the small subunit of
RT arginine-pathway carbamoyl-phosphate synthetase. Homology of the deduced
RT amino acid sequence to other glutamine amidotransferases.";
RL Eur. J. Biochem. 146:371-381(1985).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, AND MUTAGENESIS OF ASP-13.
RX PubMed=3555844; DOI=10.1016/0092-8674(87)90618-0;
RA Werner M., Feller A., Messenguy F., Pierard A.;
RT "The leader peptide of yeast gene CPA1 is essential for the translational
RT repression of its expression.";
RL Cell 49:805-813(1987).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 96604 / S288c / FY1679;
RX PubMed=9153758;
RX DOI=10.1002/(sici)1097-0061(199704)13:5<479::aid-yea104>3.0.co;2-g;
RA Poirey R., Cziepluch C., Tobiasch E., Pujol A., Kordes E., Jauniaux J.-C.;
RT "Sequence and analysis of a 36.2 kb fragment from the right arm of yeast
RT chromosome XV reveals 19 open reading frames including SNF2 (5' end), CPA1,
RT SLY41, a putative transport ATPase, a putative ribosomal protein and an
RT SNF2 homologue.";
RL Yeast 13:479-482(1997).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169874;
RA Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J.,
RA Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A.,
RA Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B.,
RA Dang V.-D., de Haan M., Delius H., Durand P., Fairhead C., Feldmann H.,
RA Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E.,
RA Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U.,
RA Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B.,
RA Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A.,
RA Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C.,
RA Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G.,
RA Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B.,
RA Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B.,
RA Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F.,
RA Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E.,
RA Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I.,
RA Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H.,
RA Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome XV.";
RL Nature 387:98-102(1997).
RN [5]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [6]
RP FUNCTION.
RX PubMed=10608810; DOI=10.1074/jbc.274.53.37565;
RA Wang Z., Gaba A., Sachs M.S.;
RT "A highly conserved mechanism of regulated ribosome stalling mediated by
RT fungal arginine attenuator peptides that appears independent of the
RT charging status of arginyl-tRNAs.";
RL J. Biol. Chem. 274:37565-37574(1999).
RN [7]
RP FUNCTION.
RX PubMed=11057443; DOI=10.1007/s002940000140;
RA Delbecq P., Calvo O., Filipkowski R.K., Pierard A., Messenguy F.;
RT "Functional analysis of the leader peptide of the yeast gene CPA1 and
RT heterologous regulation by other fungal peptides.";
RL Curr. Genet. 38:105-112(2000).
CC -!- FUNCTION: Arginine attenuator peptide (AAP) that has a regulatory role
CC in the production of arginine-specific carbamoyl phosphate synthetase.
CC Encoded by an upstream open reading frame (uORF) within the 5'-leader
CC region of arginine-specific carbamoyl phosphate synthetase small chain
CC (CPA1) mRNA, it attenuates the translation of the downstream CPA1 ORF.
CC In the presence of high concentrations of arginine, ribosomes
CC translating the uORF encoding AAP stall at the termination codon,
CC resulting in reduced translation from the downstream CPA1 initiation
CC codon. {ECO:0000269|PubMed:10608810, ECO:0000269|PubMed:11057443,
CC ECO:0000269|PubMed:3555844}.
CC -!- SIMILARITY: Belongs to the arginine attenuator peptide family.
CC {ECO:0000305}.
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DR EMBL; X01764; CAA25904.1; -; Genomic_DNA.
DR EMBL; M16690; AAA34526.1; -; Genomic_DNA.
DR EMBL; Z75210; CAA99620.1; -; Genomic_DNA.
DR EMBL; BK006948; DAA11067.1; -; Genomic_DNA.
DR PIR; S25434; S25434.
DR RefSeq; NP_014946.1; NM_001183721.1.
DR AlphaFoldDB; P08521; -.
DR BioGRID; 34690; 10.
DR DIP; DIP-1273N; -.
DR IntAct; P08521; 3.
DR MINT; P08521; -.
DR STRING; 4932.YOR302W; -.
DR PaxDb; P08521; -.
DR EnsemblFungi; YOR302W_mRNA; YOR302W; YOR302W.
DR GeneID; 854478; -.
DR KEGG; sce:YOR302W; -.
DR SGD; S000005828; YOR302W.
DR VEuPathDB; FungiDB:YOR302W; -.
DR HOGENOM; CLU_221820_0_0_1; -.
DR InParanoid; P08521; -.
DR BioCyc; YEAST:G3O-33786-MON; -.
DR PRO; PR:P08521; -.
DR Proteomes; UP000002311; Chromosome XV.
DR GO; GO:0005829; C:cytosol; IC:SGD.
DR GO; GO:0045182; F:translation regulator activity; IDA:SGD.
DR GO; GO:0006417; P:regulation of translation; IDA:SGD.
DR InterPro; IPR013203; Leader_Arg2_CPA1.
DR Pfam; PF08252; Leader_CPA1; 1.
PE 1: Evidence at protein level;
KW Leader peptide; Reference proteome.
FT PEPTIDE 1..25
FT /note="Arginine attenuator peptide"
FT /id="PRO_0000043974"
FT MUTAGEN 13
FT /note="D->N: Eliminates arginine-specific stalling of
FT ribosomes at the termination codon."
FT /evidence="ECO:0000269|PubMed:3555844"
SQ SEQUENCE 25 AA; 2979 MW; 01D494C8AFEAE4AA CRC64;
MFSLSNSQYT CQDYISDHIW KTSSH
