RL16_BACSU
ID RL16_BACSU Reviewed; 144 AA.
AC P14577;
DT 01-JAN-1990, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1995, sequence version 2.
DT 03-AUG-2022, entry version 139.
DE RecName: Full=50S ribosomal protein L16 {ECO:0000255|HAMAP-Rule:MF_01342};
GN Name=rplP {ECO:0000255|HAMAP-Rule:MF_01342}; OrderedLocusNames=BSU01230;
OS Bacillus subtilis (strain 168).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX NCBI_TaxID=224308;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=SG38;
RX PubMed=9371452; DOI=10.1128/jb.179.22.7046-7054.1997;
RA Li X., Lindahl L., Sha Y., Zengel J.M.;
RT "Analysis of the Bacillus subtilis S10 ribosomal protein gene cluster
RT identifies two promoters that may be responsible for transcription of the
RT entire 15-kilobase S10-spc-alpha cluster.";
RL J. Bacteriol. 179:7046-7054(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=168;
RX PubMed=8969501; DOI=10.1099/13500872-142-11-3039;
RA Yasumoto K., Liu H., Jeong S.M., Ohashi Y., Kakinuma S., Tanaka K.,
RA Kawamura F., Yoshikawa H., Takahashi H.;
RT "Sequence analysis of a 50 kb region between spo0H and rrnH on the Bacillus
RT subtilis chromosome.";
RL Microbiology 142:3039-3046(1996).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=168;
RX PubMed=9384377; DOI=10.1038/36786;
RA Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA Yoshikawa H., Danchin A.;
RT "The complete genome sequence of the Gram-positive bacterium Bacillus
RT subtilis.";
RL Nature 390:249-256(1997).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 39-144.
RC STRAIN=168;
RX PubMed=2508062; DOI=10.1093/nar/17.18.7469;
RA Henkin T.M., Moon S.H., Mattheakis L.C., Nomura M.;
RT "Cloning and analysis of the spc ribosomal protein operon of Bacillus
RT subtilis: comparison with the spc operon of Escherichia coli.";
RL Nucleic Acids Res. 17:7469-7486(1989).
RN [5]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 96-144.
RC STRAIN=168 / Marburg / ATCC 6051 / DSM 10 / JCM 1465 / NBRC 13719 / NCIMB
RC 3610 / NRRL NRS-744 / VKM B-501;
RX PubMed=8635744; DOI=10.1016/0378-1119(95)00757-1;
RA Suh J.-W., Boylan S.A., Oh S.H., Price C.W.;
RT "Genetic and transcriptional organization of the Bacillus subtilis spc-
RT alpha region.";
RL Gene 169:17-23(1996).
RN [6] {ECO:0007744|PDB:6HA1, ECO:0007744|PDB:6HA8}
RP STRUCTURE BY ELECTRON MICROSCOPY (3.10 ANGSTROMS) OF 1-144 WITH AND WITHOUT
RP VIRGINIAMYCIN M.
RX PubMed=30126986; DOI=10.1073/pnas.1808535115;
RA Crowe-McAuliffe C., Graf M., Huter P., Takada H., Abdelshahid M.,
RA Novacek J., Murina V., Atkinson G.C., Hauryliuk V., Wilson D.N.;
RT "Structural basis for antibiotic resistance mediated by the Bacillus
RT subtilis ABCF ATPase VmlR.";
RL Proc. Natl. Acad. Sci. U.S.A. 115:8978-8983(2018).
CC -!- FUNCTION: Binds 23S rRNA and is also seen to make contacts with the A
CC and possibly P site tRNAs. {ECO:0000255|HAMAP-Rule:MF_01342}.
CC -!- SUBUNIT: Part of the 50S ribosomal subunit.
CC {ECO:0000269|PubMed:30126986}.
CC -!- SIMILARITY: Belongs to the universal ribosomal protein uL16 family.
CC {ECO:0000255|HAMAP-Rule:MF_01342}.
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DR EMBL; U43929; AAC45963.1; -; Genomic_DNA.
DR EMBL; D50302; BAA08838.1; -; Genomic_DNA.
DR EMBL; D50303; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AL009126; CAB11899.1; -; Genomic_DNA.
DR EMBL; X15664; CAA33698.1; -; Genomic_DNA.
DR EMBL; L47971; AAB06806.1; -; Genomic_DNA.
DR PIR; B69696; B69696.
DR RefSeq; NP_388004.1; NC_000964.3.
DR RefSeq; WP_003225801.1; NZ_JNCM01000029.1.
DR PDB; 3J9W; EM; 3.90 A; BP=1-144.
DR PDB; 5NJT; EM; 3.80 A; f=1-138.
DR PDB; 6HA1; EM; 3.10 A; M=1-144.
DR PDB; 6HA8; EM; 3.50 A; M=1-144.
DR PDB; 6HTQ; EM; 4.50 A; M=1-135.
DR PDB; 6TNN; EM; 3.07 A; f=1-144.
DR PDB; 6TPQ; EM; 3.07 A; f=1-144.
DR PDB; 7AQC; EM; 2.99 A; M=1-144.
DR PDB; 7AQD; EM; 3.10 A; M=1-144.
DR PDB; 7AS8; EM; 2.90 A; Q=1-144.
DR PDB; 7AS9; EM; 3.50 A; Q=1-144.
DR PDB; 7O5B; EM; 3.33 A; j=1-144.
DR PDB; 7OPE; EM; 3.20 A; Q=1-144.
DR PDB; 7QV1; EM; 3.50 A; M=1-144.
DR PDB; 7QV2; EM; 3.50 A; M=1-144.
DR PDB; 7QV3; EM; 5.14 A; M=1-144.
DR PDBsum; 3J9W; -.
DR PDBsum; 5NJT; -.
DR PDBsum; 6HA1; -.
DR PDBsum; 6HA8; -.
DR PDBsum; 6HTQ; -.
DR PDBsum; 6TNN; -.
DR PDBsum; 6TPQ; -.
DR PDBsum; 7AQC; -.
DR PDBsum; 7AQD; -.
DR PDBsum; 7AS8; -.
DR PDBsum; 7AS9; -.
DR PDBsum; 7O5B; -.
DR PDBsum; 7OPE; -.
DR PDBsum; 7QV1; -.
DR PDBsum; 7QV2; -.
DR PDBsum; 7QV3; -.
DR AlphaFoldDB; P14577; -.
DR SMR; P14577; -.
DR STRING; 224308.BSU01230; -.
DR jPOST; P14577; -.
DR PaxDb; P14577; -.
DR PRIDE; P14577; -.
DR EnsemblBacteria; CAB11899; CAB11899; BSU_01230.
DR GeneID; 64301961; -.
DR GeneID; 66361740; -.
DR GeneID; 936807; -.
DR KEGG; bsu:BSU01230; -.
DR PATRIC; fig|224308.179.peg.126; -.
DR eggNOG; COG0197; Bacteria.
DR InParanoid; P14577; -.
DR OMA; KGAVEYW; -.
DR PhylomeDB; P14577; -.
DR BioCyc; BSUB:BSU01230-MON; -.
DR PRO; PR:P14577; -.
DR Proteomes; UP000001570; Chromosome.
DR GO; GO:0022625; C:cytosolic large ribosomal subunit; IBA:GO_Central.
DR GO; GO:0019843; F:rRNA binding; IBA:GO_Central.
DR GO; GO:0003735; F:structural constituent of ribosome; IBA:GO_Central.
DR GO; GO:0000049; F:tRNA binding; IEA:UniProtKB-KW.
DR GO; GO:0006412; P:translation; IEA:UniProtKB-UniRule.
DR CDD; cd01433; Ribosomal_L16_L10e; 1.
DR Gene3D; 3.90.1170.10; -; 1.
DR HAMAP; MF_01342; Ribosomal_L16; 1.
DR InterPro; IPR016180; Ribosomal_L10e/L16.
DR InterPro; IPR036920; Ribosomal_L10e/L16_sf.
DR InterPro; IPR000114; Ribosomal_L16.
DR InterPro; IPR020798; Ribosomal_L16_CS.
DR PANTHER; PTHR12220; PTHR12220; 1.
DR Pfam; PF00252; Ribosomal_L16; 1.
DR PRINTS; PR00060; RIBOSOMALL16.
DR SUPFAM; SSF54686; SSF54686; 1.
DR TIGRFAMs; TIGR01164; rplP_bact; 1.
DR PROSITE; PS00586; RIBOSOMAL_L16_1; 1.
DR PROSITE; PS00701; RIBOSOMAL_L16_2; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Reference proteome; Ribonucleoprotein; Ribosomal protein;
KW RNA-binding; rRNA-binding; tRNA-binding.
FT CHAIN 1..144
FT /note="50S ribosomal protein L16"
FT /id="PRO_0000062043"
FT STRAND 29..38
FT /evidence="ECO:0007829|PDB:7AS8"
FT STRAND 40..43
FT /evidence="ECO:0007829|PDB:7AS8"
FT HELIX 44..58
FT /evidence="ECO:0007829|PDB:7AS8"
FT STRAND 59..61
FT /evidence="ECO:0007829|PDB:7AQC"
FT STRAND 64..66
FT /evidence="ECO:0007829|PDB:7AQC"
FT STRAND 72..76
FT /evidence="ECO:0007829|PDB:7AS8"
FT STRAND 89..97
FT /evidence="ECO:0007829|PDB:7AS8"
FT STRAND 102..104
FT /evidence="ECO:0007829|PDB:7AS8"
FT STRAND 107..109
FT /evidence="ECO:0007829|PDB:7AS8"
FT HELIX 111..124
FT /evidence="ECO:0007829|PDB:7AS8"
FT STRAND 125..127
FT /evidence="ECO:0007829|PDB:7AS8"
FT STRAND 129..133
FT /evidence="ECO:0007829|PDB:7AS8"
SQ SEQUENCE 144 AA; 16190 MW; 648E65E6D6055343 CRC64;
MLLPKRVKYR REHRGKMRGR AKGGTEVHFG EFGIQALEAS WITNRQIEAA RIAMTRYMKR
GGKVWIKIFP SKPYTAKPLE VRMGSGKGAP EGWVAVVKPG KVLFEISGVS EEVAREALRL
ASHKLPIKTK FVKREEIGGE SNES
