AARA_DICDI
ID AARA_DICDI Reviewed; 757 AA.
AC Q54I71; Q9GSG6;
DT 19-JAN-2010, integrated into UniProtKB/Swiss-Prot.
DT 24-MAY-2005, sequence version 1.
DT 03-AUG-2022, entry version 95.
DE RecName: Full=Protein aardvark;
DE AltName: Full=Suppressor of amiB protein 16;
GN Name=aarA; Synonyms=aar, sab6; ORFNames=DDB_G0288877;
OS Dictyostelium discoideum (Slime mold).
OC Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC Dictyosteliaceae; Dictyostelium.
OX NCBI_TaxID=44689;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, DEVELOPMENTAL STAGE, SUBCELLULAR
RP LOCATION, AND DISRUPTION PHENOTYPE.
RX PubMed=11130075; DOI=10.1038/35047099;
RA Grimson M.J., Coates J.C., Reynolds J.P., Shipman M., Blanton R.L.,
RA Harwood A.J.;
RT "Adherens junctions and b-catenin mediated cell signalling in a non-
RT metazoan organism.";
RL Nature 408:727-731(2000).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=15875012; DOI=10.1038/nature03481;
RA Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT "The genome of the social amoeba Dictyostelium discoideum.";
RL Nature 435:43-57(2005).
RN [3]
RP SUBCELLULAR LOCATION, AND DISRUPTION PHENOTYPE.
RX PubMed=11792801; DOI=10.1242/jcs.114.24.4349;
RA Coates J.C., Harwood A.J.;
RT "Cell-cell adhesion and signal transduction during Dictyostelium
RT development.";
RL J. Cell Sci. 114:4349-4358(2001).
RN [4]
RP DISRUPTION PHENOTYPE.
RX PubMed=12128211; DOI=10.1016/s0925-4773(02)00152-1;
RA Coates J.C., Grimson M.J., Williams R.S.B., Bergman W., Blanton R.L.,
RA Harwood A.J.;
RT "Loss of the beta-catenin homologue aardvark causes ectopic stalk formation
RT in Dictyostelium.";
RL Mech. Dev. 116:117-127(2002).
RN [5]
RP IDENTIFICATION.
RX PubMed=18164290; DOI=10.1016/j.yexcr.2007.12.002;
RA Nagasaki A., Uyeda T.Q.P.;
RT "Screening of genes involved in cell migration in Dictyostelium.";
RL Exp. Cell Res. 314:1136-1146(2008).
CC -!- FUNCTION: Required to regulate pattern formation during multi-cellular
CC stages of development and for the formation of adherens junctions.
CC Plays a structural role during the regulation of stalk formation.
CC Involved in cell signaling. Required for spore-cell differentiation.
CC Overexpression increases number and size of cell junctions and reduces
CC spore-cell formation. {ECO:0000269|PubMed:11130075}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm. Cell junction.
CC -!- DEVELOPMENTAL STAGE: Localized at the cell junctions in the developing
CC fruiting body. {ECO:0000269|PubMed:11130075}.
CC -!- DISRUPTION PHENOTYPE: Complete loss of cell junctions. Lacks actin-
CC containing adherens junctions at the top of the stalk tube. Has weak
CC stalks causing the fruiting body to frequently collapse. Displays
CC multiple stalks appearance during late development. Shows no defects in
CC growth or division. {ECO:0000269|PubMed:11130075,
CC ECO:0000269|PubMed:11792801, ECO:0000269|PubMed:12128211}.
CC -!- SIMILARITY: Belongs to the beta-catenin family. {ECO:0000305}.
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DR EMBL; AF305417; AAG17931.1; -; mRNA.
DR EMBL; AAFI02000126; EAL62962.1; -; Genomic_DNA.
DR RefSeq; XP_636508.1; XM_631416.1.
DR AlphaFoldDB; Q54I71; -.
DR SMR; Q54I71; -.
DR STRING; 44689.DDB0191132; -.
DR PaxDb; Q54I71; -.
DR EnsemblProtists; EAL62962; EAL62962; DDB_G0288877.
DR GeneID; 8626891; -.
DR KEGG; ddi:DDB_G0288877; -.
DR dictyBase; DDB_G0288877; aarA.
DR eggNOG; KOG4199; Eukaryota.
DR HOGENOM; CLU_368213_0_0_1; -.
DR InParanoid; Q54I71; -.
DR PRO; PR:Q54I71; -.
DR Proteomes; UP000002195; Chromosome 5.
DR GO; GO:0005912; C:adherens junction; IDA:dictyBase.
DR GO; GO:0045180; C:basal cortex; IDA:dictyBase.
DR GO; GO:0005911; C:cell-cell junction; TAS:dictyBase.
DR GO; GO:0005813; C:centrosome; TAS:dictyBase.
DR GO; GO:0005737; C:cytoplasm; IDA:dictyBase.
DR GO; GO:0045294; F:alpha-catenin binding; IPI:dictyBase.
DR GO; GO:0031032; P:actomyosin structure organization; IMP:dictyBase.
DR GO; GO:0007155; P:cell adhesion; IEA:UniProtKB-KW.
DR GO; GO:0048870; P:cell motility; IGI:dictyBase.
DR GO; GO:0051642; P:centrosome localization; IMP:dictyBase.
DR GO; GO:0031154; P:culmination involved in sorocarp development; IEP:dictyBase.
DR GO; GO:0140509; P:epithelium-like organization; IMP:dictyBase.
DR GO; GO:0061245; P:establishment or maintenance of bipolar cell polarity; IMP:dictyBase.
DR GO; GO:0051645; P:Golgi localization; IMP:dictyBase.
DR GO; GO:0051495; P:positive regulation of cytoskeleton organization; IMP:dictyBase.
DR GO; GO:0008104; P:protein localization; IMP:dictyBase.
DR GO; GO:0009306; P:protein secretion; IMP:dictyBase.
DR GO; GO:0010468; P:regulation of gene expression; IEP:dictyBase.
DR GO; GO:0044671; P:sorocarp spore cell differentiation; IMP:dictyBase.
DR GO; GO:0031150; P:sorocarp stalk development; IMP:dictyBase.
DR GO; GO:0036360; P:sorocarp stalk morphogenesis; IMP:dictyBase.
DR Gene3D; 1.25.10.10; -; 2.
DR InterPro; IPR011989; ARM-like.
DR InterPro; IPR016024; ARM-type_fold.
DR InterPro; IPR000225; Armadillo.
DR InterPro; IPR036047; F-box-like_dom_sf.
DR InterPro; IPR001810; F-box_dom.
DR Pfam; PF00514; Arm; 1.
DR SMART; SM00185; ARM; 6.
DR SUPFAM; SSF48371; SSF48371; 1.
DR SUPFAM; SSF81383; SSF81383; 1.
DR PROSITE; PS50176; ARM_REPEAT; 5.
DR PROSITE; PS50181; FBOX; 1.
PE 2: Evidence at transcript level;
KW Cell adhesion; Cell junction; Coiled coil; Cytoplasm;
KW Developmental protein; Reference proteome; Repeat.
FT CHAIN 1..757
FT /note="Protein aardvark"
FT /id="PRO_0000390561"
FT DOMAIN 310..356
FT /note="F-box"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00080"
FT REPEAT 454..498
FT /note="ARM 1"
FT REPEAT 506..548
FT /note="ARM 2"
FT REPEAT 549..591
FT /note="ARM 3"
FT REPEAT 592..634
FT /note="ARM 4"
FT REPEAT 635..678
FT /note="ARM 5"
FT REPEAT 679..723
FT /note="ARM 6"
FT REGION 39..63
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 122..166
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 256..285
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 121..205
FT /evidence="ECO:0000255"
FT CONFLICT 544
FT /note="R -> K (in Ref. 1; AAG17931)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 757 AA; 85924 MW; 51A94DAAA66CC156 CRC64;
MNDCGSLFNK KLFKMNLLFK HLKLQQHLKL QQKPLLNNSS INNNINNNNN NNNNNNSNND
SNNTNTNIFN NSFLNSDLIE RLIIKFTIGY LKNNITEDYI EQILLENQNN FIKSTTTSNY
ILEENNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNSSS SSSSILSKFN KLEEDNELEL
QKKQKQQLEQ QEEELFNQFN FLEGIEDQND FLSEQETIQK IKFLIKMTAK SMSNYSSPNT
LIPSVSKTYI SPFGLSSNGS TNNHNNNNNN NHHHHSNNGN LIESSNNVNN QLNVSNYNNN
NSNHYDENNQ FDIFLIPTEM LVHLLSFLSA NDLWRISLTC KRIWYIVDVF KFWELLFEQT
CPRIYYAMQF NSRWSNPTSF QSKMILCYID RLPTDNYKNF DKSDESGQIK KIIGVMNENL
HNPMILRETC YILKRLSYRQ RKEDEHESLI ARYGGISLIL QAMKNHPYDA GVQEDACGAL
GNLTCDSPNN MGLYSNDNYL SVVEQGGIQL ILQAMKNHMM NPGVQYNTSF VLRNLARNDV
SESRVAIEGG IQSIATAMKN HPNHIGIQTQ GCGALRNLGC NDSNKVLSAK EGGIGLILRA
MRSFSSHPDL QLNGCGALRN LARNEDNKNM ISRQNGIQLV LGAMSNHPDD PDVQDEGCAA
LINLAYQDEA NEETIAREGG INLILKAMRN HPFHSGVQMQ GRGALKNLSC NPKNKLTIAR
SGGIELMNIA MQNHPNFANR FLELSRILQV ALEDGNI
