ID   AASD1_BOVIN             Reviewed;         444 AA.
AC   Q32LK1;
DT   06-FEB-2007, integrated into UniProtKB/Swiss-Prot.
DT   06-DEC-2005, sequence version 1.
DT   03-AUG-2022, entry version 79.
DE   RecName: Full=Alanyl-tRNA editing protein Aarsd1;
DE   AltName: Full=Alanyl-tRNA synthetase domain-containing protein 1;
GN   Name=AARSD1;
OS   Bos taurus (Bovine).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC   Bovinae; Bos.
OX   NCBI_TaxID=9913;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=Crossbred X Angus; TISSUE=Liver;
RG   NIH - Mammalian Gene Collection (MGC) project;
RL   Submitted (NOV-2005) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Functions in trans to edit the amino acid moiety from
CC       incorrectly charged tRNA(Ala). {ECO:0000250}.
CC   -!- COFACTOR:
CC       Name=Zn(2+); Xref=ChEBI:CHEBI:29105; Evidence={ECO:0000305};
CC       Note=Binds 1 zinc ion per subunit. {ECO:0000305};
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the class-II aminoacyl-tRNA synthetase family.
CC       Alax-L subfamily. {ECO:0000305}.
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DR   EMBL; BC109537; AAI09538.1; -; mRNA.
DR   RefSeq; NP_001033162.1; NM_001038073.1.
DR   AlphaFoldDB; Q32LK1; -.
DR   SMR; Q32LK1; -.
DR   STRING; 9913.ENSBTAP00000053479; -.
DR   PRIDE; Q32LK1; -.
DR   GeneID; 100125882; -.
DR   KEGG; bta:100125882; -.
DR   CTD; 80755; -.
DR   eggNOG; KOG2105; Eukaryota.
DR   eggNOG; KOG3158; Eukaryota.
DR   InParanoid; Q32LK1; -.
DR   OrthoDB; 910848at2759; -.
DR   Proteomes; UP000009136; Unplaced.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0004813; F:alanine-tRNA ligase activity; IEA:InterPro.
DR   GO; GO:0005524; F:ATP binding; IEA:InterPro.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0003676; F:nucleic acid binding; IEA:InterPro.
DR   GO; GO:0006419; P:alanyl-tRNA aminoacylation; IEA:InterPro.
DR   InterPro; IPR018165; Ala-tRNA-synth_IIc_core.
DR   InterPro; IPR018163; Thr/Ala-tRNA-synth_IIc_edit.
DR   InterPro; IPR009000; Transl_B-barrel_sf.
DR   InterPro; IPR012947; tRNA_SAD.
DR   Pfam; PF07973; tRNA_SAD; 1.
DR   SMART; SM00863; tRNA_SAD; 1.
DR   SUPFAM; SSF50447; SSF50447; 1.
DR   SUPFAM; SSF55186; SSF55186; 1.
DR   PROSITE; PS50860; AA_TRNA_LIGASE_II_ALA; 1.
PE   2: Evidence at transcript level;
KW   Cytoplasm; Metal-binding; Phosphoprotein; Protein biosynthesis;
KW   Reference proteome; Zinc.
FT   CHAIN           1..444
FT                   /note="Alanyl-tRNA editing protein Aarsd1"
FT                   /id="PRO_0000277464"
FT   BINDING         109
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000255"
FT   BINDING         113
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000255"
FT   BINDING         209
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000255"
FT   BINDING         213
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000255"
FT   MOD_RES         174
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:Q9BTE6"
SQ   SEQUENCE   444 AA;  48983 MW;  444466C0E1CB23FF CRC64;
     MAFRCQRDSY AREFTTTVVS CRPAELHTEE SNGKKEVLSG FQVVLEDTLL FPEGGGQPDD
     RGTINDISVL RVTRRGTQAD HFTQTPLTPG TEVQVRVDWE RRFDHMQQHS GQHLITAVAD
     DLFGLKTTSW ELGRLRSVIE LDSPTVTAEQ VAAIERSVNE KIRDRLPVNV RELSLDDPEV
     EQVRGRGLPD DHAGPIRVVT IESVDSNMCC GTHVSNLSDL QVIKILGTEK GKKNKTNLIF
     LAGNRVLKWM ERSHGIEKAL TALLKCGAED HVEAVKKLQN SSKLLQKNNL NLLRDLAVHI
     AHSLRNSPDW GGVITLHRKG GGEASWRSWA ACNFPAPFAA WSQVCALCFR KDGDSEFMNI
     IANEIGSEET LLFLTVGDEK GAGLFLLAGP AEAVETLGPR VSEVLEGKGA GKKGRFQGKA
     TKMSRRAEVQ ALLQDYISTQ SAEE