RL18_GEOSE
ID RL18_GEOSE Reviewed; 120 AA.
AC P09415;
DT 01-JUL-1989, integrated into UniProtKB/Swiss-Prot.
DT 01-JUL-1989, sequence version 1.
DT 03-AUG-2022, entry version 105.
DE RecName: Full=50S ribosomal protein L18;
DE AltName: Full=BL22;
GN Name=rplR;
OS Geobacillus stearothermophilus (Bacillus stearothermophilus).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Geobacillus.
OX NCBI_TaxID=1422;
RN [1]
RP PROTEIN SEQUENCE.
RC STRAIN=ATCC 29609 / DSM 2027 / NCA 1503 / NCIMB 8924;
RX PubMed=3542562; DOI=10.1016/0014-5793(87)81303-0;
RA Kimura J., Kimura M.;
RT "The complete amino acid sequences of the 5 S rRNA binding proteins L5 and
RT L18 from the moderate thermophile Bacillus stearothermophilus ribosome.";
RL FEBS Lett. 210:85-90(1987).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=1985969; DOI=10.1016/s0021-9258(17)35255-9;
RA Ramakrishnan V., Gerchman S.E.;
RT "Cloning, sequencing, and overexpression of genes for ribosomal proteins
RT from Bacillus stearothermophilus.";
RL J. Biol. Chem. 266:880-885(1991).
RN [3]
RP ISOLATION OF 5S RRNA-PROTEIN COMPLEXES.
RX PubMed=4567807; DOI=10.1007/bf00272091;
RA Horne J.R., Erdmann V.A.;
RT "Isolation and characterization of 5S RNA-protein complexes from Bacillus
RT stearothermophilus and Escherichia coli ribosomes.";
RL Mol. Gen. Genet. 119:337-344(1972).
RN [4]
RP PHOSPHORYLATION.
RX PubMed=10529214; DOI=10.1021/bi9914816;
RA Bloemink M.J., Moore P.B.;
RT "Phosphorylation of ribosomal protein L18 is required for its folding and
RT binding to 5S rRNA.";
RL Biochemistry 38:13385-13390(1999).
RN [5]
RP STRUCTURE BY NMR.
RX PubMed=14687565; DOI=10.1016/j.jmb.2003.11.018;
RA Turner C.F., Moore P.B.;
RT "The solution structure of ribosomal protein L18 from Bacillus
RT stearothermophilus.";
RL J. Mol. Biol. 335:679-684(2004).
CC -!- FUNCTION: This is one of the proteins that binds and probably mediates
CC the attachment of the 5S RNA into the large ribosomal subunit, where it
CC forms part of the central protuberance.
CC -!- SUBUNIT: Part of the 50S ribosomal subunit; part of the 5S rRNA/L5/L18
CC subcomplex. In B.stearothermophilus only 2 proteins, L5 and L18 have
CC been shown to be part of this subcomplex, unlike the case in E.coli and
CC T.thermophilus where L25 (TL5) is also found.
CC -!- PTM: The protein, when overexpressed in E.coli, contains a
CC phosphoserine, which is required for the protein to bind to 5S rRNA
CC (PubMed:10529214). It has been suggested, based solely on amino acid
CC conservation, that this occurs on Ser-57.
CC {ECO:0000269|PubMed:10529214}.
CC -!- SIMILARITY: Belongs to the universal ribosomal protein uL18 family.
CC {ECO:0000305}.
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DR EMBL; M57624; AAA22702.1; ALT_SEQ; Genomic_DNA.
DR PIR; B29102; R5BS8F.
DR RefSeq; WP_033008681.1; NZ_RCTK01000011.1.
DR PDB; 1OVY; NMR; -; A=1-120.
DR PDBsum; 1OVY; -.
DR AlphaFoldDB; P09415; -.
DR BMRB; P09415; -.
DR SMR; P09415; -.
DR GeneID; 58573178; -.
DR EvolutionaryTrace; P09415; -.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProt.
DR GO; GO:0005840; C:ribosome; IEA:UniProtKB-KW.
DR GO; GO:0019843; F:rRNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003735; F:structural constituent of ribosome; IEA:InterPro.
DR GO; GO:0006412; P:translation; IEA:UniProtKB-UniRule.
DR DisProt; DP00966; -.
DR HAMAP; MF_01337_B; Ribosomal_L18_B; 1.
DR InterPro; IPR005484; Ribosomal_L18.
DR InterPro; IPR004389; Ribosomal_L18_bac-type.
DR PANTHER; PTHR12899; PTHR12899; 1.
DR Pfam; PF00861; Ribosomal_L18p; 1.
DR TIGRFAMs; TIGR00060; L18_bact; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Direct protein sequencing; Phosphoprotein; Ribonucleoprotein;
KW Ribosomal protein; RNA-binding; rRNA-binding.
FT CHAIN 1..120
FT /note="50S ribosomal protein L18"
FT /id="PRO_0000131215"
FT STRAND 30..36
FT /evidence="ECO:0007829|PDB:1OVY"
FT STRAND 39..45
FT /evidence="ECO:0007829|PDB:1OVY"
FT STRAND 53..57
FT /evidence="ECO:0007829|PDB:1OVY"
FT TURN 61..63
FT /evidence="ECO:0007829|PDB:1OVY"
FT HELIX 71..88
FT /evidence="ECO:0007829|PDB:1OVY"
FT STRAND 89..91
FT /evidence="ECO:0007829|PDB:1OVY"
FT HELIX 107..117
FT /evidence="ECO:0007829|PDB:1OVY"
SQ SEQUENCE 120 AA; 13472 MW; 1E58742142882667 CRC64;
MITKVDRNAV RKKRHARIRK KIFGTTERPR LSVFRSNKHI YAQIIDDTKS ATIVSASTLD
KEFGLDSTNN IEAAKKVGEL VAKRALEKGI KQVVFDRGGY LYHGRVKALA DAAREAGLEF
